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Abha Upadhyaya, Tim D. Davis, and Narendra Sankhla

Moth bean (Vigna aconitifolia Jacqu. Marecbal cv. Jaadia) seeds were germinated in 0, 0.1, 1, or 2 μm EBL. After 72 hours, seedlings were exposed to 22 or 48C for 90 minutes. At 48C, EBL increased total electrolyte, K+, and sugar leakage from the seedlings relative to the control. Following exposure to 48C, EBGtreated seedlings bad higher malondialdebyde concentrations than controls, indicating that EBL enhanced high-temperature-induced lipid peroxidation. At 48C, EBL increased ascorbic acid oxidase activity but decreased superoxide dismutase activity relative to the control. Taken collectively, these data do not support a hypothesis that brassinosteroids confer beat shock tolerance to moth bean. Chemical name used: 24-epibrassinolide (EBL).

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Harbans L. Bhardwaj and Anwar A. Hamama

, P. Vijayakumari, K. Janardhann, K. 1994 Chemical analysis and nutritional assessment of the less known pulses, Vigna aconitifolia (Jacq.) Marechal and Vigna vexillata (L.) A. Rich Plant Foods Hum. Nutr. 45 103 111 Stephens, J.M. 1994 Bean, moth

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Abha Upadhyaya, Tim D. Davis, and Narendra Sankhla

Seeds of moth bean (Vigna aconitifolia Jacqu. Marechal cv. Jaadia) were germinated in the presence of 0, 0.1, 1, or 2 μm 24-epibrassinolide (EBL). After 72 h, cotyledons were excised and the seedlings exposed to 22 or 48 °C for 90 min. At 48 °C EBL increased total electrolyte, K+, and sugar leakage relative to the untreated control. Following exposure to 48 °C, EBL-treated seedlings had higher malondialdehyde concentrations than controls indicating that EBL enhanced high temperature-induced lipid peroxidation. At 48 °C, EBL increased ascorbic acid oxidase activity and decreased superoxide dismutase activity relative to the control. Taken together, these data do not support the hypothesis that brassinosteroids confer thermotolerance to plants. On the contrary, EBL increased high temperature-induced damage and reduced the activity of some antioxidant systems that may protect against stress-induced cellular damage.

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Rocío Leyva, Christian Constán-Aguilar, Begoña Blasco, Eva Sánchez-Rodríguez, Teresa Soriano, and Juan M. Ruíz

activities) in moth bean ( Vigna aconitifolia ) submitted to salt stress. Under summer stress conditions in the Mediterranean area, S treatment resulted in a high Pro:free Put ratio, precisely with high GDH and P5CS activities, whereas fog system treatments

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Huai-Fu Fan, Chang-Xia Du, and Shi-Rong Guo

acid can induce the expression of P5CS ( Silva-Ortega et al., 2008 ; Sripinyowanich et al., 2010 ). Vendruscolo et al. (2007) reported the effects of water deficit on wheat plants transformed with the Vigna aconitifolia P5CS cDNA and found that the