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Vida Chalavi, Zohreh Tabaeizadeh, and Pierre Thibodeau

A chitinase gene (pcht28), isolated from Lycopersicon chilense, was transferred into `Joliette' strawberry using a stipule regeneration method and Agrobacterium-mediated gene transfer technique. Stipules showed a high rate of shoot regeneration (>90%) through direct or indirect organogenesis. Stipules were cocultured with a transformation plasmid in which pcht28 was under the control of the CAMV 35S promoter. A high tendency in production of chimaeric shoots was observed. Shoots which did not show any sign of bleaching after several subcultures in kanamycin containing medium were considered as stable transformants. These shoots were successfully rooted in the presence of 50 μg·mL-1 kanamycin. Transgenic nature of the plants was confirmed by PCR as well as Southern blot analysis. Constitutive expression of the chitinase gene was demonstrated by Northern analysis. In growth chamber studies, the transgenic strawberry plants which expressed pcht28 had significantly higher resistance to Verticillium dahliae as compared to nontransgenic controls. These results demonstrate that pcht28 plays a role in defense against this fungal disease in strawberry.

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Sephra N. Rampersad

Verticillium dahliae (Kleb.) is a destructive soilborne pathogen that infects many economically important agricultural crops worldwide. Wilt caused by V. dahliae is difficult to control because the fungus can survive in the soil as

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Francisco Javier López-Escudero, Miguel Ángel Blanco-López, Carmen Del Río Rincón, and Juan Manuel Caballero Reig

Verticillium wilt of olive (VWO), incited by Verticillium dahliae Kleb., has become the most destructive disease of olive throughout the Mediterranean basin ( Al-Ahamad and Mosli, 1993 ; Blanco-López et al., 1984 ; Hiemstra and Harris, 1998

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Germán V. Sandoya, Krishna Subbarao, and Ryan Hayes

levels of resistance to race 2 are not known ( Hayes et al., 2011b ) and alternative approaches to breed crops that tolerate the disease or escape economic damage could be useful to complement existing resistance. Verticillium dahliae penetrates the

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Beiquan Mou, Steven J. Klosterman, Amy Anchieta, Elisabeth Wood, and Krishna V. Subbarao

Verticillium dahliae is a soilborne fungal pathogen that causes wilt diseases and devastating losses in many important crops ( Klosterman et al., 2009 ). The resting structures produced by V. dahliae , known as microsclerotia, can survive in soil

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Na Liu, Baoli Zhou, Xin Zhao, Bo Lu, Yixiu Li, and Jing Hao

Verticillium dahliae ). Approximately 30% to 40% of the reduction in Chinese eggplant production has been ascribed to verticillium wilt ( Wang et al., 2005 ). Symptoms of verticillium wilt on eggplant include yellow–bronze-wilted areas, mainly between the leaf

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Sahar Dabirian, Debra Inglis, and Carol A. Miles

in field production in the Pacific Northwest HortScience 48 1 7 Butterfield, E.J. De Vay, J.E. 1977 Reassessment of soil assays for Verticillium dahliae Phytopathology 67 1073 1078 Calderon, R. Lucena, C. Trapero-Casas, J.L. Zarco-Tejada, P.J. Navas

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Maria I. Villarroel-Zeballos, Chunda Feng, Angela Iglesias, Lindsey J. du Toit, and James C. Correll

soilborne pathogens affect the production of spinach leaf and seed crops ( Correll et al., 1994 ). Although Verticillium dahliae causes Verticillium wilt of more than 200 species of vegetables, field crops, and trees, the fungus was not, until recently

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Pinki Devi, Penelope Perkins-Veazie, and Carol A. Miles

Watermelon ( Citrullus lanatus ) production is limited by verticillium wilt (caused by Verticillium dahliae ) throughout Washington State, and in western Washington where watermelon are produced on a small scale for direct marketing, production is

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Ryan J. Hayes, Karunakaran Maruthachalam, Gary E. Vallad, Steven J. Klosterman, and Krishna V. Subbarao

available ( Lebeda et al., 2009 ). To achieve this, continual screening for new/additional resistances is necessary because new diseases emerge or change. Verticillium wilt caused by Verticillium dahliae Kleb. is a serious disease of lettuce that was first