The American cranberry (Vaccinium macrocarpon Ait.) was genetically transformed with the bar gene, conferring tolerance to the phosphinothricin-based herbicide glufosinate. Plants of one `Pilgrim' transclone grown under greenhouse conditions were significantly injured by foliar treatments of 100 mg·L-1 glufosinate, although the injury was less severe when compared to untransformed plants. However, the same transclone grown outdoors in coldframes survived foliar sprays of 500 mg·L-1 glufosinate and higher, while untransformed plants were killed at 300 mg·L-1. Actively growing shoot tips were the most sensitive part of the plants and at higher dosages of glufosinate, shoot-tip injury was evident on the transclone. Injured transgenic plants quickly regrew new shoots. Shoots of goldenrod (Solidago sp.) and creeping sedge (Carex chordorrhizia), two weeds common to cranberry production areas, were seriously injured or killed at 400 mg·L-1 glufosinate when grown in either the greenhouse or coldframe environment. Stable transmission and expression of herbicide tolerance was observed in both inbred and outcrossed progeny of the above cranberry transclone. Expected segregation ratios were observed in the outcrossed progeny and some outcrossed individuals demonstrated significantly enhanced tolerance over the original transclone, with no tip death at levels up to 8000 mg·L-1. Southern analysis of the original transclone and two progeny selections with enhanced tolerance showed an identical banding pattern, indicating that the difference in tolerance levels was not due to rearrangement of the transgene. The enhanced tolerance of these first generation progeny was retained when second generation selfed progeny were tested.
Eric L. Zeldin, Thomas P. Jury, Rodney A. Serres, and Brent H. McCown
Luping Qu, James Polashock, and Nicholi Vorsa
Putative transgenic cranberry plants have been achieved via Agrobacterium-mediated transformation. Leaf explants were transformed with a supervirulent Agrobacterium tumefaciens strain EHA 105, harboring the binary vector P35SGUSint and nptII selectable marker genes. Inoculation of precultured explants (≈10 days on regeneration medium) coupled with sonicasion improved transformation efficiency significantly. Adventitious shoots were directly regenerated from explants. Putative transformed shoots were identified by being kanamycin-resistant and GUS-positive. Stable GUS gene expression (turning blue) could be detected within 1 h of incubation at 37 °C. Confirmation of transformation by molecular analysis is in progress. Eight putative transgenic cranberry plants were obtained. All appeared morphologically normal. This appears to be the first success in achieving cranberry transformed plants by Agrobacterium-mediated method. Optimizing the transformation system is ongoing.
Deborah L. Allan, Bruce D. Cook, and Carl J. Rosen
The effect of N form and solution pH on the carboxylic and phenolic acid content of cranberry (Vaccinium macrocarpon Ait. cv. Searles) shoots and roots was determined in a greenhouse experiment. The predominant carboxylic acids measured were malate and citrate. Protocatechuic acid was the dominant phenolic acid detected. Total organic acid concentrations were unaffected by N form supplied. In shoots, higher total concentrations of organic acids were found at pH 4.5 than at 6.5 in the shoot, but there was little pH effect in the roots.
Kim Patten, John Wang, Fred Katz, Don Riemer, Chuck Kusek, and Herb Hopen
Tolerance of cranberry (Vaccinium macrocarpon Ait.) at different phenological stages to the postemergent broadleaf herbicide clopyralid (0.21 or 0.42 kg a.i./ha) was evaluated in Washington, New Jersey, Massachusetts, and Wisconsin. Tolerance varied among states, rates, and application times. Applications made during early shoot growth, especially at the high rate, usually resulted in the most crop injury (leaf cupping and epinasty and reduced yield); while applications at the low rate made after vegetative development occurred usually resulted in less or no injury. No phytotoxicity occurred when applications were made before shoot growth (Washington and New Jersey). Chemical name used: 3,6-dichloro-2-pyridinecarboxylic acid (clopyralid).
Ricardo Cesped-Ruiz* and Bingru Huang
The American cranberry often undergoes drought stress during the summer. However, the physiological response of this species to drought is not well understood. This study was designed to determine the effects of drought on two commercial cranberry cultivars of high potential yield, `Ben Lear' and `Stevens', during a vegetative stage. The plants were subjected to drought for 15 days in a greenhouse. Soil water content, leaf water content, leaf photosynthetic rate, stomatal conductance, transpiration, differential leaf-air temperature, photochemical efficiency (Fv'/Fm') and the actual PSII efficiency (deltaF/Fm') decreased in those plants subjected to drought. Drought reduced differential leaf-air temperature at day 6 of treatment and stomatal conductance and transpiration starting at day 9 and photosynthetic rate at day 13. Drought decreased leaf water content at day 14 and Fv'/Fm' and PSII efficiency at day 15. Our results indicated that cranberry plants in vegetative stage were sensitive to drought for both cultivars and stomatal conductance was the most sensitive parameter among those examined for both cultivars.
T.R. Roper, E.J. Stang, and G.M. Hawker
Carolyn DeMoranville and Joan Davenport
The relationship between yield and applied N in cranberry has been investigated. Cultivar was important in determining optimum seasonal N rate. Sustained production for the hybrid `Stevens' required an annual seasonal total of N at up to 67 kg·ha–1, higher than was optimal for native selections `Early Black' and `Howes'. High N rates were associated with increased fruit rot and vine overgrowth. Optimum N rate varied within cultivar, likely due to variation in soil conditions, but soil N test results have not correlated well with subsequent yield in cranberry. Soil organic matter content can predict potential N release, but plant response must also be taken into account. To refine N rate recommendations, plant characteristics that might predict N requirements/status of cranberry were investigated. A standard of 0.9% to 1.1% N in August tissue has been established for cranberry. To find characteristics that could be used earlier, we surveyed 30 sites for percentage of N in tissue, length of new growth, SPAD chlorophyll meter ratings, fertilizer N use, and yield. Length of new growth could be used as an indicator of cranberry N status from June until bloom, being positively correlated with subsequent yield. The SPAD meter proved to be a viable alternative to in-season monitoring of tissue N during June and July. Readings below proposed standard values indicated the need for N fertilizer if vegetative growth was in the standard range. Thus, the easily determined factors of upright length and SPAD rating could be used to refine fertilizer rates during the active growing season, while tissue testing for percentage of N could be used as a “report card” on the fertilizer program at the end of the season.
T.R. Roper, A.R. Krueger, C.J. DeMoranville, N. Vorsa, J. Hart, and A.P. Poole
Nitrogen fertilizer application is a universal practice among cranberry growers. Cranberries only use ammonium nitrogen sources. This study was undertaken to discover how quickly cranberries in the field would take up fertilizer-derived ammonium nitrogen. Ammonium sulfate labeled with 15N was applied in field locations in Oregon, Massachusetts, New Jersey, and Wisconsin. Samples of current season growth were collected daily for 7 days beginning 24 hours after fertilizer application. In all cases 15N was detectable in the plants from treated plots by 24 hours following application. Additional nitrogen was taken up for the next 3 to 5 days depending on the location. With the exception of Oregon, the maximum concentration of 15N was found by day 7. Oregon was the coolest of the sites in this research. To determine a temperature response curve for N uptake in cranberry, cranberry roots were exposed to various temperatures in aeroponics chambers while vines were at ambient greenhouse temperatures. The optimum temperature for N uptake by cranberry vines was 18 to 24 °C. This research suggests that ammonium fertilizers applied by growers and irrigated into the soil (solubilized) are taken up by the plant within 1 day following application. Soil and root temperature is involved in the rate of N uptake.
Bernadine C. Strik and Arthur Poole
Timing and severity of pruning in a 30-year-old commercial `McFarlin' cranberry (Vaccinium macrocarpon Ait.) bed were studied. Treatments in 1989 and 1990 consisted of early or late pruning and heavy, moderate, light, or no pruning. Yield component data were collected in Fall 1989 and 1990, just before harvest. Time of pruning did not affect yield components. In 1989, the unpruned and lightly pruned vines had a higher total plant fresh weight, fewer berries, higher berry yield, longer and more fruiting uprights, and fewer nonfruiting uprights (U,) compared with moderately or heavily pruned vines. Average length of UN and anthocyanin content of berries in 1989 were not influenced by pruning. In 1990, the effects of pruning severity were similar to 1989. In 1990, unpruned vines had a lower percent fruit set and berries contained less anthocyanin than pruned vines. Annual pruning with conventional systems in use decreases yield.
Bernadine C. Strik, Teryl R. Roper, Carolyn J. DeMoranville, Joan R. Davenport, and Arthur P. Poole
This research was undertaken to document the extent of biennial bearing in flowering uprights by American cranberry (Vaccinium macrocarpon Ait) cultivar and growing region. Seven cultivars were studied: three found in all states considered (Massachusetts, New Jersey, Wisconsin, Oregon), two common to Massachusetts and New Jersey, and two other commercially grown cultivars, one each from Wisconsin and Oregon. There were significant cultivar, region, and cultivar × region interaction effects for both percent return bloom (%RB) and percent return fruit (%RF). Percent RB ranged from 74% for `Ben Lear' in Wisconsin to 14% for `Howes' in New Jersey. `Ben Lear' differed the most in %RB among regions, from 74% in Wisconsin to 14% in Massachusetts. However, in some regions, especially in Wisconsin, many blossoms did not set viable fruit. There was no significant difference in %RB among cultivars grown in Massachusetts or Oregon; however, cultivars grown in these regions did differ in %RF.