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Jelka Šustar-Vozlič, Marko Maras, Branka Javornik, and Vladimir Meglič

There is a long tradition of common bean cultivation in Slovenia, which has resulted in the development of numerous landraces in addition to newly established cultivars. The genetic diversity of 100 accessions from the Genebank of the Agricultural Institute of Slovenia (AIS) were evaluated with amplified fragment length polymorphism (AFLP) markers and phaseolin seed protein. Twenty-seven standard accessions of known Mesoamerican and Andean origin, 10 wild Phaseolus vulgaris accessions and two related species, P. coccineus L. and P. lunatus L., were also included. Ten AFLP primer combinations produced 303 polymorphic bands, indicating a relatively high level of genetic diversity. Based on the marker data, unweighted pair group method with arithmethic mean (UPGMA) analysis and principal coordinate analysis (PCoA) all P. vulgaris accessions were separated into three well-defined groups. Two groups consisted of accessions of Mesoamerican and Andean origin, while the third was comprised of only four wild P. vulgaris accessions. A set of Slovene accessions formed a well-defined sub-group within the Andean cluster, showing their unique genetic structure. These data were supported by phaseolin analysis, which also revealed additional variants of “C” and “T” phaseolin types. The results are in agreement with previous findings concerning diversification of common bean germplasm introduced in Europe.

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Hannah R. Swegarden, Craig C. Sheaffer, and Thomas E. Michaels

bean ( Phaseolus vulgaris ) cultivars included in the 2013–14 yield trials. Seed descriptors (seed size, shape, weight, coat color, coat patter, corona color, length, width, and thickness) were evaluated from 2012 original seed stock (n = 15) and 2013

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Kyle M. VandenLangenberg, Paul C. Bethke, and James Nienhuis

and Hopp, 1961 ). In general, if sweetness is perceived in a vegetable, even mildly, it may be enough to encourage consumption ( Dinehart et al., 2006 ). Sensory panelists, who preferred dry bean ( Phaseolus vulgaris L.) and edamame [ Glycine max (L

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Kerrie McDaniel and David Lightfoot

Physiological differences between cttokinins are well documented. An explanation for these differences is that cytokinins differentially regulate genes. Gene response has been analyzed in cell culture and organized tissue of Phaseolus vulgaris L. cv. Great Northern. Two novel cDNAs, L-221 and L-22, have been identified that are cytokinin responsive. In leaf tissue L-221 was repressed by zeatin, benzyladenine and thidiazuron at 50 μM. In suspension cell culture mRNA abundance of L-221 remained constant regardless of cytokinin treatment. By contrast, the abundance of L-22 mRNA was increased differentially by treatment with each of the 3 cytokinins in leaf tissue. Suspension cells analyzed for expression of L-22 after cytokinin treatment also showed differential gene expression. S-1 Nuclease Protection Assays revealed that gene expression is a transient phenomenon dependent upon the time of cytokinin application and cytokinin concentration. Callus bioassays showed that dihydrozeatin and O-glucosylzeatin gave greater responses than the co-application of zeatin and dihydrozeatin or zeatin and O-glucosylzeatin. The conjugate and the reduction derivative also gave greater responses than zeatin alone. Effects of dihydrozeatin and O-glucosylzeatin on gene expression will be reported.

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Jesse Vorwald and James Nienhuis

Nuña beans are a type of common bean ( Phaseolus vulgaris L.) native to the Andean region of South America that possess the unusual property of popping when exposed to heat ( National Research Council, 1989 ). Popped nuñas are a snack food similar

Open access

Wesley Gartner, Paul C. Bethke, Theodore J. Kisha, and James Nienhuis

al., 2012 ). Days after flowering is an additional measure of maturity that has been used for comparisons among genotypes, although variation in rates of maturity could result in biased comparisons. Fruits of Phaseolus vulgaris contain a single

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MiAe Cho, Brandon M. Hurr, Jiwon Jeong, Chaill Lim, and Donald J. Huber

Green or common beans ( Phaseolus vulgaris L.) are harvested at a physiologically immature stage of development. Growth is rapid at the time of harvest and beans exhibit comparatively high respiration rates, even when held at low temperatures

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Jesse Vorwald and James Nienhuis

Nuña beans ( Phaseolus vulgaris L.) are a type of common bean that possess the unusual property of popping when exposed to heat ( National Research Council, 1989 ). Popping of nuñas results from the expansion of the cotyledons and produces a nut

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Diana Carolina Núñez-López, Augusto Ramírez-Godoy, and Hermann Restrepo-Díaz

desarrollo de métodos de muestreo para Trialeurodes vaporariorum (Westwood) Hemiptera: Aleyrodidae) en habichuela y frijol ( Phaseolus vulgaris L.) Rev. Colomb. Entomol. 31 161 169 Cardona, C. Rodríguez, G. Adela, P. Pedro, C. 1993 Umbral de acción para el

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Alexander D. Pavlista, Dipak K. Santra, James A. Schild, and Gary W. Hergert

In dry bean ( Phaseolus vulgaris L.), growth form, i.e., determinate vs. indeterminate, and growth habit, i.e., upright/erect/bushy vs. viny/prostate, are among the most important characteristics for classifying cultivars from an agronomic