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Jalil Dejampour, Islam Majidi, Solmaz Khosravi, Sevil Farhadi, and Atena Shadmehr

great importance. The HS314 rootstock is a hybrid between almond and peach ( Prunus amygdalus × P. persica ), which is well adapted to drought conditions ( Dejampour et al., 2005 ). This rootstock that has been bred in Sahand Horticultural Research

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Thomas G. Beckman and William R. Okie

Differences in chilling and post-rest heat requirements of various stonefruits were investigated through the use of cuttings collected from field grown trees. Materials studied included P. angustifolia Marsh, P. besseyi Bailey, P. maritima Marsh, P. persica (L.) Batsch (`Agua 6-4', `Flordaking', `Pi Tao', `Redhaven', `Redskin', and `Ta Tao'), P. umbellata Ell. and a Japanese type plum (`Byrongold'). Cuttings were collected after natural leaf fall and shortly after the onset of of chill hour accumulation. Cuttings were stored at 4°C. Groups of cuttings were removed from storage after various amounts of chilling and allowed to develop at 16, 21 or 27°C. Cuttings were observed for both vegetative and flower bud break. Magnitude of differences in chilling and post-rest heat requirements and their implications in the breeding of peaches for low and moderate chill areas will be discussed.

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Alberto C. O. Pinto, David H. Byrne, and Suzanne M. D. Rogers

SH and MS media, sucrose concentrations (6% and 10%) and types of support (0.25% Gelrite, vermiculite and filter paper bridge) were compared in a factorial experiment to determine the effects on growth of immature embryos from peach cultivar B611505. Embryos were measured at the beginning of the experiment (control) and all treatments were kept in the dark at room temperature, for 40 days. Although gelrite, over all media treatments, increased embryos wet weight by 66%, the embryos were soft and succulent and their dry weight increased only 37%. Vermiculite support, on the other hand, increased wet and dry weights by 63% and 79%, respectively. Less embryo growth occurred with MS medium and filter paper bridge. Except for vermiculite and SH medium, 10% sucrose was more effective than 6% in increasing embryo growth.

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Thomas M. Gradziel and Steven A. Weinbaum

The regulation of anther dehiscence by relative humidity (RH) was assessed for detached anthers and detached whole flowers from a limited selection of apricot (Prunus armeniaca L.), peach [P. persica (L.) Batsch], and almond [P. dulcis (Mill.) D.A. Webb, syn. P. amygdalus Batsch; P. communis (L.) Arcangeli, non Huds.] genotypes, as well as an almond X peach F2 progeny. Dehiscence was evaluated at 33, 64, 87, 93 and 97% RH for detached anthers, and at 33, 64 and 97% RH for whole detached flowers. Anther dehiscence was suppressed with increasing RH for all genotypes. Apricot anthers showed the greatest dehiscence at low RH and measurable dehiscence at high RH even when detached. Anther dehiscence in almond appeared more suppressed than in apricot at all RH levels tested, being completely suppressed by high RH in detached anthers. Peach genotypes exhibited the full range of variability between apricot and almond patterns. Evidence for transgressive segregation of RH-controlled anther dehiscence was observed in the occurrence of cleistogamy in an almond × peach F2 progeny. Rates of anther dehiscence were approximately linear with change in RH in detached anthers but exhibited a more buffered, step-wise response when detached whole flowers were tested. Results are consistent with field observations, and highlight the low but measurable risk of cleistogamy in these species, as well as opportunities to modify the breeding systems and crossing environments to facilitate controlled hybridization, and to reduce pollination vulnerability to adverse environments.

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Zuguo Cai, Wenfang Zeng, Liang Niu, Zhenhua Lu, Guochao Cui, Yunqin Zhu, Lei Pan, Yifeng Ding, and Zhiqiang Wang

(Zhengzhou, Nanjing, and Beijing). These 20 accessions were representative samples of five species ( P. persica , P. davidiana , P. kansuensis , P. ferganensis , and P. mira ) that were established on the core collection during initial investigations in

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Elizabeth E. Rogers and Craig A. Ledbetter

expected to contribute to increasing the frequency of ALSD. However, because peach × almond ( P. persica × P. dulcis ) hybrids are being increasingly used as rootstocks in California almond orchards, susceptibility of such hybrids are of concern. One

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Ke Cao, Lirong Wang, Gengrui Zhu, Weichao Fang, Chenwen Chen, and Pei Zhao

development of additional molecular markers within these regions. Materials and Methods Plant material. Seven P. persica rootstock cultivars and two P. kansuensis cultivars were used in this study. Resistance to RKN was evaluated in 1998 at Zhengzhou Fruit

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Annick Moing, Jean-Luc Poëssel, Laurence Svanella-Dumas, Michèle Loonis, and Jocelyne Kervella

Prunus davidiana (Carr.), a wild species with poor fruit quality that is related to peach [Prunus persica (L.) Batsch], is used as a source of resistance to pests and diseases in peach breeding programs. Two genotypes of P. davidiana were studied for fruit biochemical composition and compared to three genotypes of P. persica (`Summergrand', `Bailey' and `Pamirskij'), and two P. persica × P. davidiana hybrids. Fruit of P. davidiana clones had higher malic acid, neochlorogenic and cryptochlorogenic acid and lower sucrose concentrations than fruit of all P. persica genotypes, even poor-quality Bailey. Differences in biochemical composition could be related to sensory evaluation. P. persica × P. davidiana hybrids had intermediate values between their parents for neochlorogenic acid concentration. They were similar to the P. persica parent for total soluble sugar, malic and citric acid, amino acid and catechin concentrations, indicating possible rapid progress for fruit quality in a breeding program.

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Chunxian Chen and William R. Okie

the National Center for Biotechnology Information (NCBI). The sequences, designated as CPpe, CPka, and CPmu, respectively, were NC_014697 from P. persica cv. Nemared ( Jansen et al., 2011 ), NC_023956 from P. kansuensis (submitted to the NCBI by S

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Jose X. Chaparro, Ronald R. Sederoff, and Dennis J. Werner

Total cellular DNA has been extracted from leaves and\or seed of Prunus dulcis, P. persica, P. mira, P. davidiana, P. persica subsp. ferganensis, and P. triloba. Chloroplast restriction fragments have been visualized by Southern blot analysis using heterologous probes from a petunia chloroplast library. Analysis of preliminary data separates the species into three groups. The first contains P. dulcis, P. mira, and P. davidiana; the second P. kansuensis, P. persica, and P. persica subsp. ferganensis; and the third P. triloba.

PCR amplification using oligos for cytosolic glyceraldehyde-3-phosphate dehydrogenase yields genomic fragments approximately 1kb in size from P. dulcis and P. triloba. Sequence analysis will be performed to determine species relationships at the gene level.