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Robert L. Jarret

Patterns of diversity among thirty diploid clones of banana (Musa acuminata Colla.), collected in Papua New Guinea and the surrounding islands between 1987 and 1989, were examined genetically using the polymerase chain reaction (PCR) and random primers, to detect random amplified polymorphic DNA (RAPDs). PCR products were visualized on ethidium bromide stained agarose gels. Twenty of 60 random primers examined detected RAPDS in CTAB-extracted genomic DNA. Banding patterns ranged from very simple (1 or 2 bands/gel) to very complex (more than 20 bands/gel). All 30 Musa clones were distinguishable from each other based on their unique RAPD banding pattern. Principal component analysis (PCA) revealed several clusters of closely related clones within the materials examined. However, these clusterings were not correlated with either the geographic origin or the morphological characteristics of the clones. A role of the use of RAPDs in germplasm characterization is discussed.

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Rufino Perez and Randolph M. Beaudry

We hypothesized that the blocking of O2 influx and CO2 efflux in banana (Musa acuminata) by sealing nearly 100% of the pores over a fraction of the surface would generate a modified internal atmosphere in a manner similar to fruit coatings that cover 100% of the banana surface but only block a fraction of the pores. This hypothesis was based on the observation made by previous workers that the flesh of mature green bananas has insignificant resistance to O2 diffusion relative to the resistance imposed by the skin of the fruit. We modified the O2 diffusion pathway in bananas by covering, beginning at one end, ¼, ½, ¾, and ⅞ of the fruit surface with paraffin, which sealed essentially 100% of the surface where it was applied. Large end-to-end O2 and CO2 gradients developed within coated fruit, relative to the uncoated control, suggesting that the diffusive resistance in the pulp was not insignificant. Since the large gradients of O2 generated caused uneven ripening, using fractional coatings may help analyze gas exchange properties, but it is not suitable for commercially controlling ripening of bananas.

Open access

Tao Dong, Fang-cheng Bi, Yong-hong Huang, Wei-di He, Gui-ming Deng, Hui-jun Gao, Ou Sheng, Chun-yu Li, Qiao-song Yang, Gan-jun Yi, and Chun-hua Hu

used to deliver plasmids to establish an efficient genetic transformation system in ECS of Musa acuminata cv. Baxi. Materials and Methods Experimental materials. ECS of Musa acuminata cv. Baxi were developed from immature male flowers using the

Open access

Chao Zhou, Haide Zhang, Yixing Li, Fenfang Li, Jiao Chen, Debao Yuan, and Keqian Hong

Banana ( Musa acuminata ) is a commercially valuable climacteric fruit with ethylene peak. Once ripening is initiated, senescence is irreversible and rapid, as manifested by softening, peel spotting, and fungal decay, and leads to a short shelf

Free access

Cai-Hong Jia, Ju-Hua Liu, Zhi-Qiang Jin, Qiu-Ju Deng, Jian-Bin Zhang, and Bi-Yu Xu

et al., 2011a ). Banana ( Musa acuminata L. AAA group cv. Brazilian) is a typical climacteric fruit, which is largely cultivated in tropical and subtropical regions. The pattern of ethylene production during banana fruit ripening differs from other

Open access

Ricardo Goenaga, Brian Irish, and Angel Marrero

/default.aspx#ancor > Goenaga, R. Irizarry, H. González, E. 1993 Water requirement of plantains ( Musa acuminata x Musa balbisiana AAB) grown under semiarid conditions Trop. Agr. 70 3 7 Goenaga, R. Irizarry, H. 1998 Yield of banana grown with

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Ren-jun Feng, Li-li Zhang, Jing-yi Wang, Jin-mei Luo, Ming Peng, Jun-feng Qi, Yin-don Zhang, and Li-fang Lu

western Pacific ( Davey et al., 2013 ). Cultivated bananas and plantains are mainly triploids that derive from intraspecific hybridizations within Musa acuminata (A genome) and interspecific hybridizations with Musa balbisiana (B genome) ( Davey et al

Open access

Ricardo Goenaga, Brian Irish, and Angel Marrero

Agrícolas 2021 Programa de banano y plátano 1 Jan. 2021. < http://www.fhia.org.hn/htdocs/banano_y_platano.html > Goenaga, R. Irizarry, H. González, E. 1993 Water requirement of plantains ( Musa acuminata x Musa balbisiana AAB) grown under

Free access

Piyada A. Tantasawat, Apinya Khairum, Kitiya Arsakit, Oythip Poolsawat, Paniti Pornbungkerd, and Chitpan Kativat

The effects of culture media on growth and proliferation of ‘Earsakul’ dendrobium (Dendrobium) protocorm-like bodies (PLBs) were evaluated in a two-step culture. After culturing on each of the four first step media for 4 months and on each of the four second step media for 4 months, the greatest total PLB fresh weight, increase in number of PLBs and growth rates were obtained when using Vacin and Went medium 1 (VW1) in both culture steps compared with those in 15 other medium combinations. Starting from 0.5 g of PLBs, culturing on VW1 for 8 months achieved a total of 415.25 g of PLBs, a multiplication rate of 830-fold. The supplementation of tomato (Solanum lycopersicum) and substitution of ‘Hom Thong’ banana [Musa acuminata (AAA group)] with ‘Khai’ banana [M. acuminata (AA group)] in this new medium promoted growth and proliferation of dendrobium PLBs 2.4-fold over the control medium, suggesting its usefulness in commercial micropropagation.

Free access

Jiao Chen, De-bao Yuan, Chao-zheng Wang, Yi-xing Li, Fen-fang Li, Ke-qian Hong, and Wang-jin Lu

Many reports indicate that an abundance of really interesting new gene (RING) play key roles in regulating defense responses against abiotic and biotic stresses in plants. In this study, the cloning and functional characterization of a RING gene, MaRING2, in banana (Musa acuminata) fruit are reported. MaRING2 belongs to the NEP1-interacting protein (NIP) RING-H2 finger protein family. Gene expression profiles revealed that MaRING2 was cold responsive and induced by abscisic acid (ABA) treatment during cold storage. In this study, the MaRING2 under control of the Cauliflower mosaic virus 35S (CaMV 35S) promoter was transformed to tobacco (Nicotiana benthamiana) using agrobacterium (Agrobacterium tumefaciens)-mediated transformation. The resultant MaRING2-overexpressing transgenic plants (35S:MaRING2) exhibited significantly increased tolerance to low temperatures and were hypersensitive to exogenous ABA in terms of germination and early seedling growth. In addition, overexpression of MaRING2 enhanced the expression of stress-responsive genes under normal (before cold stress) or cold conditions. These results demonstrate the biological role of MaRING2 in conferring cold tolerance. Taken together, these results suggest that MaRING2, a C3H2C3-type RING protein, is a positive regulator of the ABA-dependent stress response.