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R.J. Griesbach and R.N. Bhat

Tetraploid forms of lisianthus [Eustoma grandiflorum (Grisebach) Schinners] were produced by applying 0.5% (w/v) aqueous colchicine to seedling apices. The tetraploids had stronger stems and reduced plant stature, which provided better support to flowers than diploids.

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Fernando de la Riva, Pilar Carolina Mazuela, Juan Eugenio Álvaro, and Miguel Urrestarazu

Eustoma grandiflorum flowers as influenced by peracetic acid in the holding solution in comparison with hypochlorite sodium. PAAs are presumed to reduce the accumulation of bacteria in water and flower stems while, at the same time, limiting environmental

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R.J. Griesbach

The environment can affect the intensity of flower color in Eustoma grandiflorum. Low light and alkaline pH within the growing cell can lead to reduced color intensity. Two independent causes are responsible for the decrease in the intensity of flower color. 1) Older flowers were more alkaline than freshly opened flowers. A 7% increase in pH was related with a 10% reduction in color intensity. 2) Flowers that open under low light were paler than those opening under high light intensity. A 25% decrease in light intensity was related to a 30% reduction in the concentration of anthocyanin and a 40% reduction in color intensity.

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David B. Rubino

Segregating progenies from controlled pollinations of Eustoma grandiflorum Griseb. were investigated to determine the inheritance of diaphorase (DIA) and glucoses-phosphate isomerase (GPI) isozymes. Phenotypic data supported the hypotheses that DIA1 is tetrameric and is controlled by a single locus with two alleles (Dia1-1 and Dia1-2) and that GPI1 is dimeric and also is controlled by a single locus with two alleles (Gpi1-1 and Gpi1-2). Examination of isozyme phenotypes for over 70 cultivars of E. grandiflorum revealed polymorphism for DIA1 and GPI1. These isozymes may be useful for marker-assisted selection and cultivar identification.

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K. Ohta, A. Taniguchi, N. Konishi, and T. Hosoki

The effects of chitosan treatment on plant growth and flower quality of Eustoma grandiflorum (Raf.) Shinn. were investigated. The application of a soil mix of chitosan (1%, w/w) at sowing time remarkably enhanced growth, whereas coating seed with 0.1% chitosan in lactate was ineffective. Plants grown in the chitosan-treated soil flowered 15 days earlier than did control plants, and the number and weight of cut flowers produced were greater than for control plants or plants from chitosan-treated seed. Chemical name used: poly-(1→4)-β-D-glucoseamine (chitosan).

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Kiyoshi Ohkawa, T. Yoshizumi, M. Korenaga, and K. Kanematsu

The effects of seedling age and temperature regimes and durations on the reversal of Eustoma grandiflorum (Raf.) Shin. heat-induced rosette formation were clarified. When E. grandiflorum seedlings were grown in a natural-light phytotron (600-800 μmol·m-2·s-1) for 4 weeks at 33/28C (12-h day/12-h night) from germination to the four true-leaf stage, the optimum temperature and duration required to break rosette formation was 15C for 4 weeks with continuous illumination (35 μmol·m-2·s-1). However, when seedlings were grown for 12 weeks at 33/28C from germination to the eight true-leaf stage. shoot elongation required 6 weeks at 10C.

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Katsumi Ohta, Mika Suzuki, Shingo Matsumoto, Takashi Hosoki, and Nobuo Kobayashi

We previously reported that growth of lisianthus [Eustoma grandiflorum (Raf.) Shinn.] seedlings is accelerated by amending the growing medium with 1% (w/w) chitosan. This finding prompted us to search for organic nitrogenous other substances like chitosan which could accelerate seedling growth. Seeds of E. grandiflorum `Peter blue line 2'were sown in a sandy loam growing medium containing 1% (w/w) chitosan, tryptone, casein, collagen or gelatin. At eleven weeks after sowing, leaf length and width, fresh and dry weights of the shoots and roots of twelve plants were determined for each treatment. Eleven weeks after sowing, the leaves at the fifth node had expanded in the chitosan, tryptone and collagen treatments while the leaves of the third node had not yet expanded in control plants. Fresh and dry weights of shoots and roots were significantly greater for plants grown in media amended with chitosan or tryptone. Percent nitrogen (N) and potassium (K) in the shoots and roots and percent phosphorus (P) in the shoots was greater only in the N side dressing treatment. The nitrate nitrogen (NO3-N) concentration was significantly greater in media amended with tryptone or collagen compared to the other treatments.

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Lucina Gómez-Pérez, Luis Alonso Valdez-Aguilar, Alberto Sandoval-Rangel, Adalberto Benavides-Mendoza, Rosalinda Mendoza-Villarreal, and Ana María Castillo-González

ornamental horticultural production systems. Lisianthus [ Eustoma grandiflorum (Raf.) Shinn.], a species native to the arid zones of the southern United States and northern México, is an ornamental plant of increasing demand. Valdez-Aguilar et al. (2013

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Ya-Ching Chuang and Yao-Chien Alex Chang

Eustoma grandiflorum is a popular and important cut-flower crop as a result of its long, multiflowered inflorescence, and varying flower colors, sizes, and shapes. The vase life of Eustoma cut flowers can exceed 2 weeks under favorable conditions

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Brent K. Harbaugh and John W. Scott