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Andrea Swanberg and Wenhao Dai

genes is important to elucidate the function of future-cloned resistant genes and interaction between phytoplasma and plant hosts. A regeneration system is a prerequisite for recovering transgenic plants. Many factors, including genotype ( Choi et al

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Wenhao Dai and Cielo Castillo

, elite traits cannot be efficiently integrated into new plants ( Tobutt, 1993 ). In vitro mutation and gene transformation are being explored ( Rose et al., 2000 ). The objective of this study was to determine factors influencing regeneration of two

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Ya-Long Qin, Xiao-Chun Shu, Wei-Bing Zhuang, Feng Peng, and Zhong Wang

resistant and high yielding, can be imported into the cultivated plant to obtain high-quality varieties through genetic engineering ( Jin et al., 2004 ). Regeneration systems have been successfully established in many varieties of Solanum L., including

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Rochelle R. Beasley and Paula M. Pijut

for conservation, mass propagation, and genetic improvement of black ash. Thus, the aim of the present study was to develop a complete protocol for plant regeneration of black ash from hypocotyls for further use in genetic transformation studies

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Ilse-Yazmín Arciniega-Carreón, Carmen Oliver-Salvador, María-Guadalupe Ramírez-Sotelo, and Carlos Edmundo Salas

, the aim of this work is to establish a protocol for in vitro regeneration of I. sonorae to serve as a plant repository, and to define the growth conditions for subsequent establishment of cell-suspension cultures aiming the production of secondary

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Takahiro Tezuka, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, and Masayuki Oda

advantage of the inherent regenerative ability of plants, a new method, termed the complete decapitation method (CDM) by Johkan et al. (2008c) , was developed for mass propagation in tomato and poinsettia ( Harada et al., 2005 ; Nielsen et al., 2003

Open access

Sadiye Hayta, Mark A. Smedley, Jinhong Li, Wendy A. Harwood, and Philip M. Gilmartin

( Schween and Schwenkel, 2003 ) regenerated plantlets from pedicle explants. Although reasonably efficient, the obligate requirement for floral material to initiate culture greatly limits this system. The limited amount of floral material produced per plant

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Masafumi Johkan, Genjirou Mori, Kazuhiko Mitsukuri, Keiichirou Mishiba, Toshinobu Morikawa, and Masayuki Oda

plants grown in vivo, was reported as a new vegetative propagation method in tomato plants ( Harada et al., 2005 ). The authors reported that 79 shoots regenerated from calluses on the stock plant 36 d after decapitation. Propagation efficiency using the

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Li Xu, Suzhen Huang, Yulin Han, and Haiyan Yuan

). Somatic embryogenesis is the fastest system of plant regeneration and generally considered to be prerequisite for genetic transformation ( Jeknic et al., 1999 ; Karami et al., 2006 ). Therefore, several protocols for iris regeneration via somatic

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G.G. Ning and M.Z. Bao

; Olaya et al., 2000 ). For P. mume , an in vitro culture system has been reported for the induction of callus, but plant regeneration was not achieved ( Liu and Chen, 1999 ). Although it is possible to conduct traditional breeding programs in P. mume