Plant tissue culture can induce a variety of genetic and epigenetic changes in regenerated plantlets, a phenomenon known as somaclonal variation. Such variation has been widely used in the ornamental foliage plant industry as a source for selection of new cultivars. In ornamental aroids alone, at least 63 somaclonal-derived cultivars have been released. In addition to morphological differences, many somaclonal aroid cultivars can be distinguished by amplified fragment length polymorphism (AFLP) analysis. However, a few cultivars have no detectable polymorphisms with their parents or close relatives by AFLP fingerprints. It is postulated that DNA methylation may be involved in the morphological changes of these cultivars. In this study, methylation-sensitive amplification polymorphism (MSAP) technique was used to study DNA methylation in selected somaclonal cultivars of Alocasia, Aglaonema, Anthurium, Dieffenbachia, Philodendron, and Syngonium. Results showed that polymorphisms were detected in the somaclonal cultivars, suggesting that DNA methylation polymorphisms may associate with tissue culture-induced mutation in ornamental aroids. This is the first study of methylation variation in somaclonal variants of ornamental foliage plants. The results clearly demonstrate that the MSAP technique is highly efficient in detecting DNA methylation events in somaclonal-derived cultivars.
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Jinggui Fang, Chih Cheng Chao, Richard J. Henny, and Jianjun Chen
Zhanao Deng, Fahrettin Goktepe, and Brent K. Harbaugh
Caladiums are ornamental aroids widely grown as pot plants or used in landscapes as accent or border plants. Commercial pot caladium plants are produced by forcing tubers, while dry tubers are available for garden or landscape planting ( Evans et
Teresa E. Seijo, Natalia A. Peres, and Zhanao Deng
Caladium ( Caladium × hortulanum Birdsey) is an ornamental aroid grown as landscape and potted plants. They are valued for their colorful foliage that comes in various leaf shapes and coloration patterns containing shades of red, pink, white
Zhe Cao, Shunzhao Sui, Qian Yang, and Zhanao Deng
Cultivated caladium is an ornamental aroid produced for container or hanging basket plants or grown in landscapes for their variably shaped, colorful foliage. The ornamental value of caladium plants, to a great extent, depends on their leaf
Zhanao Deng, Fahrettin Goktepe, Brent K. Harbaugh, and Jinguo Hu
for other ornamental aroids ( Chen et al., 2004a , 2004b , 2004c ; Henny, 1977 ). Reduced genetic diversity could result in increased vulnerability to new diseases and pests and in reduced genetic variation for cultivar development. Some genetic
Zhanao Deng and Brent Harbaugh
Poster Session 7—Ornamental Plant Breeding 18 July 2005, 1:15–2:00 p.m. Poster Hall–Ballroom E/F
Zhanao Deng and Brent K. Harbaugh
resulted in diverse and intriguing foliar coloration patterns in these plants ( Henny, 1988 ). These coloration patterns have been a major contributing component to the ornamental and/or economic value of a number of important ornamental aroids ( Henny and
Richard J. Henny, Jianjun Chen, and Terri A. Mellich
cultivars 466 472 Janick J. Whipkey A. Trends in new crops and new uses ASHS Press Alexandria, VA Henny, R.J. Norman, D.J. Chen, J. 2004 Progress in ornamental aroid breeding Ann. Mo. Bot. Gard. 91 465 473 Royal Horticultural Society The Royal Horticultural
W.L. Chen and D.M. Yeh
carrying and spreading disease. Tissue culture is preferable for rapid multiplication of healthy plants. However, endogenous microbial contamination is known to be one of the most serious problems in tissue culture of ornamental aroids, including
Mohammed Elsayed El-Mahrouk, Yaser Hassan Dewir, and Yougasphree Naidoo
. Subsequently, tissue culture is considered the best method to get stock aroids free from endogenous contaminations ( Elsheikh et al., 2013 ; Taylor and Knauss, 1978 ). In vitro propagation methods are used for production of ornamental plants to meet the