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The effect of incorporated sphagnum peatmoss and minimal fertilization on the establishment and subsequent growth of rabbiteye blueberries (Vaccinium ashei Reade) was determined in 4 field studies conducted on typical fine sandy loam, upland mineral soils in south Mississippi. Incorporated peatmoss increased plant vigor, plant height, shoot weight, leaf chlorophyll level, and fruit yield and reduced chlorosis symptoms. First- and second-year plant growth and second-year fruit yields were reduced by either slow-release or fast-release granulated fertilizer. Soluble fertilizers produced less plant damage than granulated fertilizers but no more plant growth than no fertilization. There was a close association between over-fertilization and cholorosis symptoms.

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Abbreviations: A, apparent net photosynthesis; Berryland, Berryland sand soil high in organic matter; CYV, canopy volume; DMP, dry-matter production; E, transpiration; g L , leaf conductance of water; Galestown, Galestown sandy clay loam soil; IC

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Data from a four-parent diallel, involving one highbush (Vaccinium corymbosum L.) clone and three interspecific hybrids grown on mineral soil unamended with organic matter, were analyzed to determine combining ability effects for six traits: plant size, berry size, the number of days between flowering and fruiting (# DBF&F), the ratio of total fruit weight to canopy volume (TFW: CYV), days to fruit ripe, and yield. General combining ability effects were significant for all characters tested, except yield and berry size in 1984. Specific combining ability effects were significant for plant size in 1983, #DBF&F in 1984, TFW: CYV in 1984, and berry size in 1985. Vigorous and productive highbush cultivars can be developed for mineral soils by using the interspecific clones from this study and their selected recombinant to combine the genes for plant vigor with the high-quality fruit traits of highbush cultivars.

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Distribution of radiolabeled assimilates was examined at various intervals after 1 hour of light or dark 14CO2 fixation by leaves or developing fruit of grapefruit (Citrus paradisi Macf.) so that the fate of assimilates from each source could be assessed at sequential stages of fruit growth. Exported products of both light and dark 14CO2 fixation in leaves were deposited primarily in juice tissues of fruit even during periods of substantial dry weight accumulation by peel. Fruit photosynthesis, however, gave rise to assimilates that remained almost entirely in the peel (flavedo and albedo) even 7 days later, regardless of dry matter increases by other tissues. Products of dark 14CO2 fixation by intact fruit were recovered in all tissues but predominated in the peel of young fruit vs. juice tissues at later stages of growth. Comparison of dry matter gains and 14C-labeled assimilate distribution indicated that fruit photosynthesis likely contributed substantially to development of peel but not juice sacs. Data on dark 14CO2 fixation were consistent with its suggested involvement in organic acid synthesis by juice sacs.

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A variety of organic materials such as humic substances, seaweed extracts (SWE), organic matter, and amino acids are being used as fertilizer supplements in commercial turfgrass management. Among them, SWE and humic acid (HA) are widely used in various biostimulant product formulations. These compounds have been reported to contain phytohormones and osmoprotectants such as cytokinins, auxins, polyamines, and betaines. Manufacturer claims are that these products may supplement standard fertility programs by reducing mineral nutrient requirements while improving stress tolerance. There is a lack of season-long, field-based evidence to support these claims. This study was conducted to investigate the influence of monthly field applications of SWE, HA, and high and low seasonal fertilization regimes on the physiological health of fairway-height creeping bentgrass (Agrostis stolonifera L.). Plots were treated monthly with SWE at 16 mg·m-2 and HA (70% a.i.) at 38 mg·m-2 alone, or in combination, and were grown under low (20 kg·ha-1/month) or high nitrogen (50 kg·ha-1/month) fertilization regimes during 1996 and 1997. Endogenous antioxidant superoxide dismutase (SOD) activity, photochemical activity (PA), and turf quality were measured in July of each year. Superoxide dismutase activity was increased by 46% to 181%, accompanied by a PA increase of 9% to 18%, and improved visual quality of bentgrass in both years. There was no significant fertilization × supplement interaction. Although not part of our original objectives, it was noted that significantly less dollar spot (Sclerotinia homoeocarpa F.T. Bennett) disease incidence occurred in supplement-treated bentgrass. Our results indicate that increased SOD activity in July due to SWE and/or HA applications improved overall physiological health, irrespective of fertilization regime. This suggests that these compounds may be beneficial supplements for reducing standard fertilizer and fungicide inputs, while maintaining adequate creeping bentgrass health.

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The chemical composition of the lowbush blueberry (Vaccinium angustifolium Aiton) cultivars Blomidon, Cumberland, and Fundy were examined at three stages of fruit maturity, before and after refrigerated storage, in a 2-year study. There were differences (P< 0.001) related to maturity and cultivar in berry fresh weight, percentage dry matter, fruit firmness, percentage soluble solids, titratable acidity, and the concentration of sugar, acids, and anthocyanins. Among the nine organic and phenolic acids measured, seven acids varied among the maturity groups and eight varied among the cultivars. Between the 2 years of study there was a 60% difference in total fruit acid content as well as in the relative amounts of each acid. The 2-year mean profile of lowbush blueberry acids was distinctly different from that recently reported for highbush (Vaccinium corymbosum L.) and rabbiteye blueberries (Vaccinium ashei Reade). The level of certain acids as well as the concentration of anthocyanins increased during cold storage. Estimation of sugar concentration by percentage soluble solids overestimated sugar concentration by 3070. Acid measurement by titration underestimated acid content as measured by HPLC by 61%. Results of this study illustrate the variation in the chemical composition of lowbush blueberry fruit among cultivars, maturities, and seasons, and can be used to compare lowbush blueberries with other Vaccinium species.

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The effect of cover-crop management on growth and yield of `Bravo' cabbage (Brassica oleracea var. Capitata L.), `Market Pride' tomato (Lycopersicon esculentum Mill.), and `Mustang' snap bean (Phaseolus vulgaris L.) was determined. Each fall, `Wheeler' winter rye (Secale cereale L.) and `Oregon Crown' hairy vetch (Vicia villosa Roth) were interseeded. The following spring, the cover crops were killed by either applying glyphosate and mowing (CC-G) or mowing and disking (CC-D). Trifluralin was preplant incorporated into bare ground as a conventional tillage (CT) treatment. In 1992 and 1993, a chicken (Gallus gallus L.) based fertilizer was applied to half the subplots. The greatest snap bean and cabbage yields were in CT. The system with the greatest tomato yields varied. In 1991, the greatest tomato yields were in the CT treatment, while in 1992 yields were greatest in the CT and CC-D treatments, and in 1993 the greatest yields were in CT and CC-G. Cabbage yields were greater in the fertilized than the unfertilized treatments. In 1992, infestations of diamondback moth, imported cabbageworm, and cabbage looper were greater in CT than in the CC-G treatment. Three years of the CC-G treatment increased soil organic matter from 3.07% to 3.48% and increased soil pH from 6.30 to 6.51, while neither changed in the CT. Chemical names used: N-(phosphonomethyl) glycine (glyphosate); 2,6-dinitro-N,N-dipro`pyl-4-(trifluoromethyl) benzenamine (trifluralin).

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To understand the genetics that control pod Ca concentration in snap beans, two snap bean (Phaseolus vulgaris L.) populations consisting of 60 genotypes, plus 4 commercial cultivars used as checks, were evaluated during Summers 1995 and 1996 at Hancock, Wis. These populations were CA2 (`Evergreen' × `Top Crop') and CA3 (`Evergreen' × `Slimgreen'). The experimental design was an 8×8 double lattice repeated each year. No Ca was added to the plants grown in a sandy loam soil with 1% organic matter and an average of 540 ppm Ca. To ensure proper comparison for pod Ca concentration among cultivars, only commercial sieve size no. 4 pods (a premium grade, 8.3 to 9.5 mm in diameter) were sampled and used for Ca extractions. After Ca was extracted, readings for Ca concentration were done via atomic absorption spectrophotometry. In both populations, genotypes and years differed for pod Ca concentration (P = 0.001). Several snap bean genotypes showed pod Ca concentrations higher than the best of the checks. Overall mean pod Ca concentration ranged from a low of 3.82 to a high of 6.80 mg·g-1 dry weight. No differences were detected between the populations. Significant year×genotype interaction was observed in CA2 (P = 0.1), but was not present in CA3. Population variances proved to be homogeneous. Heritability for pod Ca concentration ranged from 0.48 (CA2) to 0.50 (CA3). Evidently enhancement of pod Ca concentration in beans can successfully be accomplished through plant breeding.

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This study was conducted to compare various orchard groundcover management systems (GMSs)—including a crownvetch “living mulch” (CNVCH), close-mowed (MWSOD) and chemically growth-regulated (GRSOD) sodgrasses, pre-emergence (NDPQT) and two widths of post-emergence (GLY1.5 and GLY2.5) herbicides, hay-straw mulch (STMCH), and monthly rototillage (tilled)—during the first 6 years in a newly established apple (Malus domestica Borkh.) planting. Mean soil water potential at 5 to 35 cm deep varied substantially among treatments each summer, and treatment × year interactions were observed. During most growing seasons from 1986 to 1991, soil water availability trends were STMCH > NDPQT > GLY2.5 > GLY1.5 > tilled > GRSOD > MWSOD > CNVCH. Soil organic matter content increased under STMCH, CNVCH, and MWSOD and decreased under NDPQT and tilled treatments. Water infiltration and saturated hydraulic conductivity after 4 years were lower under NDPQT and tilled, and soil under STMCH and GRSOD retained more water per unit volume at applied pressures approximating field water capacity. Mid-summer soil temperatures at 5 cm deep were highest (25 to 28C) in tilled and NDPQT plots, intermediate (22 to 24C) under GRSOD, and lowest (16 to 20C) under CNVCH and STMCH. These observations indicate that long-term soil fertility and orchard productivity may be diminished under pre-emergence herbicides and mechanical cultivation in comparison with certain other GMSs.

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Effects of lateral movement of soil from tile lines based on soil and plant analysis, plus the effects of applications of ZnSO4 and MnSO4 on the concentrations of Zn, Mn, and other elements in the leaf blades of snap bean plants (Phaseolu vulgaris, var. humilis cv. Bush Blue Lake-47) and on the yield of snap bean pods at harvest were determined. Snap beans were grown across the tile lines and fertilized with five rates of ZnSO4 and MnSO4 fertilizers applied in a band at planting time. The soil decreased in pH and Ca and Mn content and increased in organic matter and Zn with distance from the tile lines. The leaf blades decreased in concentration of Ca and increased in concentrations of Mg, Zn, and Mn with distance from the tile lines. High rates of Zn and Mn fertilizers were required to obtain medium concentrations of Zn and Mn in plants grown over or near the tile lines. Concentrations of 24 to 30 μg of Zn per gram dry weight and 60 to 90 μg of Mn per gram dry weight in the leaf blades of the snap bean plants were adequate for highest yield of pods. Zinc sulfate at a rate of 0.5 to 0.7 g of Zn/m2 produced the highest yield of pods at a distance of 3 m from the tile lines. Applied together, ZnSO4 and MnSO4 produced a yield response similar to application of only ZnSO4. Twenty years after installation of the tile lines, the effects of the tile lines on soil and leaf analysis and yield of pods of snap beans plants extended 2 to 3 m in each direction from the tile lines, indicating that the soil moved laterally.

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