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differ with plant species. One characteristic of anther development is the accumulation of nutrients, generally polysaccharides or neutral lipids, in the pollen pool to fuel the subsequent pollen ontogeny, germination on the stigma surface, and growth of
Abstract
Seven mutant maize genotypes with sweet corn backgrounds and 4 commercially grown sweet corn cultivars were harvested from 18-45 days after pollination (DAP). The lipids were extracted with chloroform-methanol 2: 1 (v/v) and separated on a silicic acid column into neutral lipids, glycolipids and phospholipids. Mutant genotypes influenced lipid development while inbred lines seemed important in amount of oil synthesized by the kernel. Crude oil and neutral lipids increased from 18-45 DAP while phospholipid and glycolipid values (mg/10 g fresh weight basis) were higher 23-28 DAP which is the prime processing time. During the developmental period studied, neutral lipids, phospholipids and glycolipids amount to 63, 20, and 12% respectively of the crude oil extracted. About 5% of the crude oil was lost in the separation procedure.
analogues where basic fuchsin combines to display red color ( Dahlqvist et al., 1965 ). These sections were then counter-stained with 0.3% (w/v) Sudan Black B (19966-4; Sigma-Aldrich) for 30 min at 60 °C to detect neutral lipid droplets. The Sudan Black B
Abbreviations: DTT, dithiothreitol; FAME, fatty acid methyl ester, NL, neutral lipids; PL, polar lipids; PVPP, polyvinylpolypyrrolidone. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations
Abbreviations: ASG, acylated steryl glycoside; DGDG, digalactosyldiacylglycerol; FS, free sterols; GL, galactolipids; GlyL, glycolipid; MGDG, monoga-lactosyldiacylglycerol; NL, neutral lipid; PA, phosphatidic acid; PC, phosphatidylcholine; PE
Abstract
The study evaluated the roles of storage carbohydrates and neutral lipids in the success of Colorado blue spruce (Picea pungens Englemann ‘Hoopsi’) grafts. These scions do not require photosynthesis nor receive photosynthates from the rootstock during union development. Carbohydrate and neutral lipid contents, along with respiration and scion water relations, were measured during union development. Stored carbon compounds were sufficient to supply the needs of the scion during the 9 weeks of union development. Estimates of carbohydrate use indicated that decreases in sugar content (bark and needle) were insufficient to account for more than 25% of the estimated respiration. The results indicate that the quantity of carbon storage compounds is not a factor in graft success. We propose that neutral lipids may be the major carbon reserve of the scion during graft formation.
Abstract
Major leaf alkanes (C29-C33) of 2 scions on 10 rootstocks of citrus were examined by gas chromatography. A small but definite effect of the rootstock on the alkane profiles of the scions was observed. The effect of rootstock on alkane patterns in juice sacs was very small. Rootstock affected the fatty acid patterns of total and neutral lipids as well as of triglycerides and sterol esters.
Abstract
Seven mutant maize genotypes with sweet corn backgrounds and 4 commercially grown sweet com cultivars were harvested from 18-45 days after pollination (DAP). The lipids were extracted, separated into major lipid fractions, transesterified and measured as methyl esters of palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2) and linolenic (18:3) acids by gas liquid chromatography (GLC). Neutral lipids had a low ratio of polyunsaturates to monounsaturates (1.7:1), i.e., linoleic and linolenic to oleic fatty acids. Glycolipids had the highest proportion of linoleic and linolenic acids. Phospholipids tended to be more saturated than other fractions because of their high proportion of palmitic acid. All fatty acids in the neutral lipid fraction increased on an absolute basis with advancing maturity. Fatty acids in the glycolipid and phospholipid fractions generally peaked at 28 DAP on a mg fatty acid/g corn wet weight basis and then decreased with increased maturity. The percentage of oleic acid in the glycolipid fraction doubled from 12-24 percent during the 18-45 DAP period. Experimental lines and commercial cultivars contained 18.4, 1.4, 21.5, 57.1 and 2.1 percent respectively of palmitic, stearic, oleic, linoleic and linolenic acid over the maturity ranges studied.
inhibition, photosynthesis and respiration anomalies, water deficiency, an imbalance of carbon and nitrogen metabolism, lipid peroxidation, a modulation of the levels of hormones and primary and secondary metabolites as well as protein and nucleic acid
). Histochemical and biochemical analyses of the stigmatic exudate show that it is heterogeneous and composed of lipids, polysaccharides, and proteins. Cresti et al. (1982) evaluated the stigma of Citrus limon and using SEM evaluations of fresh tissues