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  • holoploid 2C genome size x
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. Taxonomic, trademark, accession, and source information for Syringa source material used in the current study. Flow cytometry. Flow cytometry was used to assess holoploid (2C) genome size (relative to an internal standard) for each individual taxon in the

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content of the pea reference ( Doležel et al., 1998 ; Greilhuber et al., 2007 ) where 2C represents the holoploid, or complete, genome size. Cytological analysis. Both meiotic (flower bud) and mitotic (root tip) tissues were used to determine chromosome

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). Two internal standards were selected for this study to represent the wide range of genome sizes existing in Salvia . These were Solanum lycopersicum ‘Stupické’, which has a holoploid 2C genome size of 1.96 pg, and Pisum sativum ‘Ctirad’, with a 2C

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trade as the market name. For simplicity, only market names (cultivar or trademark) will be used hereafter. Table 1. Ploidy and relative holoploid 2C genome size in cultivars of Hibiscus syriacus . Flow cytometry. Holoploid (2C) relative genome sizes

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relative holoploid 2C DNA content following the methods of Jones et al. (2007) . Genome sizes were determined by comparing mean relative fluorescence of each sample with an internal standard, Pisum sativum ‘Ctirad’, with a known genome size of 8.76 pg

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numbers and holoploid (2C) genome sizes (measured in picograms) for Acer species evaluated in this study. Polyploidy, or whole-genome duplication, can be used to facilitate wide hybrid crosses ( Sanford, 1983 ) or to develop sterile ornamental cultivars

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were assigned accession numbers. Table 1. Source and collection information for 67 Cotoneaster accessions. Genome sizing. Holoploid (2C) genome sizes were determined by flow cytometry (CyFlow PA; Partec, Münster, Germany) and comparison of mean

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counts per sample. Holoploid, 2C genome size was calculated as: 2C = genome size of standard × (mean fluorescence value of sample/mean fluorescence value of standard). Cytology was conducted on an individual accession from each subgroup, determined in the

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size and ploidy among taxa may provide insight into cross-compatibility in the current study. Cytological studies describe lilacs to be primarily diploids ( Darlington and Wylie, 1956 ) with holoploid genome sizes near 2.5 pg ( Olszewska and Osiecka

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clean the cytometer between each run. The 2C DNA contents were calculated as follows: 2C = DNA content of standard × (mean fluorescence value of sample ÷ mean fluorescence value of the standard). Expt. 1: Estimating genome sizes of plant reference

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