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Abstract

Cultured shoot tips and lateral buds from greenhouse-grown rose (Rosa hybrida L. cv. Improved Blaze) proliferated multiple shoots on a basal medium (MS salts, vitamins, glycine, sucrose, and agar) supplemented with 3.0 mg/liter 6-benzylamino purine (BA) and 0.3 mg/liter indoleacetic acid (IAA). A 3-fold multiplication was achieved from freshly explanted terminal shoot tips or lateral buds after 8 weeks. Reculture of in vitro-derived shoots onto the same medium resulted in a 6-fold increase in 8 weeks. Roots could be initiated from about 50% of these shoots after transfer to a medium containing 0.3 mg/liter IAA and 0 or 0.3 mg/liter BA. Regenerated plants were successfully transferred to soil after 2 weeks.

Open Access

Abstract

Dierama latifolium N.E. Br. was propagated in vitro using corm cultures. Shoots were induced from corm explants when grown on solidified Murashige and Skoog (MS) medium supplemented with 30 g/liter sucrose, 100 mg/liter meso-inositol, and 0 or 0.5 mg/liter NAA. Shoot proliferation was not improved by the addition of BA to the initial culture medium. Multiple shoots were induced by transferring those produced in vitro, to a modified MS medium supplemented with 0.5 or 1.0 mg/liter BA. Rooting of these shoots was induced by subculturing single excised shoots, 5-10 mm in height, either a hormone-free basal medium or a BM supplemented with 0.5 or 1.0 mg/liter NAA. Utilizing this technique, about 90 plants could be produced from a single corm within 12 months. Chemical names used: 1-napthalenacetic acid (NAA); N-(phenylmethyl)-1H-purin-6-amine(BA).

Open Access

Abstract

Lateral buds excised from unrooted cuttings of Maranta leuconeura E. Moor. ‘Kerchoviana’, prayer plant, were cultured on Linsmaier and Skoog (LS) medium supplemented with various combinations of N6-benzylaminopurine (BA), kinetin, α-anaphthaleneacetic acid (NAA), and 3-indoleacetic acid (IAA) for the production of multiple shoots and complete plants. Of 48 combinations of growth regulators, 4 produced the most vigorous, well-formed shoots after a culture period of 6 weeks: 2.0 mg·liter-1 kinetin, 0.2 mg·liter-1 BA, 0.2 mg·liter-1 BA plus 0.1 mg·liter-1 NAA, and 2.0 mg·liter-1 kinetin plus 1.0 mg·liter-1 IAA. Enhanced shoot production occurred when shoots were transferred at 12-week intervals and when they were maintained on 0.2 mg liter-1 BA. Complete plants with strong, fibrous root systems were produced after the shoots were transferred to basal medium lacking growth regulators for 3-4 weeks.

Open Access

Guava (Psidium guajava L.) is an exceptional source of vitamin. C. It is also considered to be the most important cultivated species of the Myrtel family. Shoot tip and stem node were taken from seedling germinated in Murashige and Skoog medium (MS) and cultured in the same medium supplemented with 1-3mg/l benzylaminopurine (BA) and 0.1mg/l naphthaleneacetic acid (NAA) or 0.2-2mg/l thidiazuron (TDZ) and 0.1mg/l NAA. Multiple shoots (4-6) were obtained in 4-5 weeks from culture in 1-2mg/l BA and 0.1mg/l NAA, while TDZ caused abnormal shoot growth. Shoots were rooted successfully with 100% frequency in MS medium containing 2mg/l indolebutyric acid and further elongation of shoots was achieved in MS medium, supplemented with lg/l activated charcoal. Regenerated plantlets were successfully established in soil.

Free access

Vegetative long-shoot buds, greenwood stems, and immature needles of 20-year-old western larch (Larix occidentalis Nutt.) were cultured to induce multiple bud formation. Explants were collected year-round and cultured on a modified Schenk and Hildebrandt (SH) medium containing 6-benzyladenine (BA) at 0, 1, 5, 10, 50, or 100 μm. Multiple buds were produced on buds and stems with terminal meristems, but not on needles or stem sections. The induction of de novo buds and development of axillary buds required BA at 1 to 10 μm; higher concentrations of BA were less effective. More explants formed multiple buds on SH than on modified Murashige and Skoog (MS) media. Multiple buds formed on more buds and stems excised during the growing season than from dormant buds. Buds cultured on media containing gibberellin died within 6 weeks; auxin caused bud elongation but no multiple buds formed. Chemical names used: N-[(trichloromethyl)thio]-4-cyclohexene-1,2-dicarboximide (captan); 6-benzyladenine (BA); 1H-indole-3-butyric acid (IBA); 1H-indole-3-acetic acid (IAA); gibberellin (GA4+7).

Free access

Abstract

Clonal multiplication of carnation (Dianthus caryophyllus L.) was accomplished in three stages: 1) shoot tip culture initiation stage, 2) shoot multiplication stage, and 3) rooting stage. The culture medium for the initiation stage was examined by comparing various inorganic salt mixtures, vitamin mixtures, carbohydrates, growth regulators, agars, pH’s, and additional supplements for their effect on growth and development of multiple shoots from shoot tips. When shoot tips (ca. 1 mm high) were grown on a modified Murashige and Skoog medium with 10 µM kinetin and 1 µM NAA, apical dominance was counteracted and morphologically normal shoots proliferated rapidly. Transferring these cultures after 4 wk to 100 ml flasks (one per flask) with 50 ml of same medium without agar and supplements, and with the kinetin concentration reduced to 2.5 µM, resulted in an average per original shoot tip of 28 shoots over 2 cm in height being produced in another 3 weeks. These shoots were rooted in BR-8 blocks or Jiffy-7 peat pellets under intermittent mist. Plantlets rooted in these supports were transferred easily to greenhouse conditions. Incorporation of carnation micropropagation into a pathogen-free propagative stock program should not be difficult, and might prove beneficial even if large scale use is limited by economic considerations.

Open Access

Abstract

Rapid clonal propagation of Alnus glutinosa was achieved in vitro using lateral buds excised from greenhouse grown, juvenile stock plants. Multiple shoot development occurred in 50% of the cultures after the first subculture (7–8 weeks after initial explanting) using a low salt, woody plant medium containing 1 μM 6-benzylaminopurine (BA). Microshoots were removed from pro-liferating tissues and rooted in a conventional potting medium under high humidity prior to establishment in the greenhouse.

Open Access

Abstract

N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP) has been used to promote multiple shoot formation in previous tissue culture studies with ericaceous plants (1, 3-7). Fordham et al. (3), however, found that (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buren-1-ol (zeatin) was the most effective cytokinin for stimulating shoot proliferation of cultured Exbury azalea (Rhododendron sp.). This study was conducted to determine if highbush blueberry is similar to Exbury azalea in its response to zeatin.

Open Access

Abstract

Inflorescence explants of Liriope muscari Bailey Variegata’ and Ophiopogon jaburan Lodd. ‘Variegatus’ produced multiple shoots in vitro on a modified Murashige and Skoog medium via callus. In subsequent studies with L. muscari‘ Variegata’, there was no difference in floret and scape explant growth, while rhizome and modified root explants were either contaminated or failed to grow. Distal floret explants produced more shoots than proximal explants but there were no significant positional differences in scape explants. In liquid culture 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.5 mg/liter promoted the greatest fresh weight and shoot and root length. Benzylamino purine (BA) at 0.1 mg/liter increased the number of shoots while decreasing the number of roots. Agitation of liquid cultures increased callus fresh weight.

Open Access

Abstract

Shoot tips and stem nodes of Asclepias erosa Torr., cultured on a modified (0.5 × major salts) Murashige and Skoog (MS) medium containing 0.54 µm (0.1 mg/liter) NAA and 44.4 µm (10 mg/liter) BA, produced multiple shoots in 5 weeks. Subcultures of the individual shoots on the same medium produced 5-12 new shoots 4 weeks later. Rooting of the resultant shoots was best accomplished by preculturing them for 48 hr on MS medium containing 246 or 492 µm (50 or 100 mg/liter) IB A prior to subculturing for 4 weeks on MS medium devoid of growth regulators. The rooted cultures were established successfully in soil. Chemical names used. NAA: 1-naphthaleneacetic acid. BA:N-(phenylmethyl)-lH-purin-6-amine. IBA: lH-indole-3-butanoic acid.

Open Access