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diploids. For those diploids with relatively high accumulations of total flavonoids and phenolics, further accumulations with chromosome doubling are difficult. Our research demonstrated that the accumulation of metabolites in triploid loquat is affected by

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.R. Clarindo, W.R. 2017 From chromosome doubling to DNA sequence changes: Outcomes of an improved in vitro procedure developed for allotriploid “Híbrido de Timor” ( Coffea arabica L. × Coffea canephora Pierre ex A. Froehner) Plant Cell Tissue Organ Cult. 131

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. Van Laere, K. Eeckaut, T. Leus, L. Van Huylenbroeck, J. 2011 Mitotic chromosome doubling of plant tissues in vitro Plant Cell Tissue Organ Cult. 104 359 373 https://doi.org/10.1007/s11240-010-9786-5 Dirr, M.A 2009 Manual of woody

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. picturatum ), which suggests a possible chromosome doubling or polyploidization in these CCG-3 species. However, when genome size and chromosome number were both considered, conflicts emerged as for how caladium genome size and chromosome number had evolved

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. domestica , a hexaploid, originated as a hybrid between Prunus cerasifera , a diploid, and Prunus spinosa , a tetraploid, via either chromosome doubling of the hybrid triploid or as a product of unreduced gametes from both parents. Zohary (1992) proposed

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with hybrid necrosis in Aronia , Pyrus , and closely related genera. Future studies could attempt chromosome doubling of intergeneric hybrid seedlings, mutagenesis or genome editing of lethal genes to alleviate the symptoms of hybrid necrosis. In

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determine what role the slow bolting biennial nature of L. virosa plays in resistance. L. virosa has been used successfully in lettuce breeding but requires the use of colchicine-induced chromosome doubling, bridge crossing, and/or embryo rescue ( Eenink

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. Wentworth, J. Sieber, V.K. 2003 Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability Theor. Appl. Genet. 107 1195 1200 Krüssmann, G. 1981 The complete book of roses Timber Press Portland, OR Leus, L. 2005

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, H.K. Cho, H.R. Kim, K.J. Kim, K.S. 2005 Comparison of pollen morphology in interspecific hybrid lilies after in vitro chromosome doubling Acta Hort. 673 639 643 Rosenfeldt, S. Galati, B.G. 2005 Ubisch bodies and pollen ontogeny in Oxalis articulata

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plants Annu. Rev. Ecol. Syst. 29 467 501 10.1146/annurev.ecolsys.29.1.467 Ranney, T.G. 2006 Polyploidy: From evolution to new plant development Proc. Intl. Plant Propagators Soc. 56 604 607 Rose, J.B. Kubba, J. Tobutt, K.R. 2000 Chromosome doubling in

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