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developmental stage tended to increase the rate of anther dehiscence. The fertility of the pollen grains was evaluated by in vitro germination. ‘Senshu’ showed a pollen germination rate of more than 80% ( Table 5 , Fig. 5A ), whereas the rate for 95P5 and 92P3

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the northern Great Plains region Crop Sci. 42 2018 2024 Reid, S.D. Koski, A.J. Hughes, H.G. 1993 Buffalograss seedling screening in vitro for NaCl tolerance HortScience 28 536 Reiten, J.G. Lee, C.W. Cheng, Z.M. Smith, R.C. 1992 Germination salt

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. Rindera umbellata in its natural habitat, in vitro, and acclimated to greenhouse and field conditions. ( A ) Population of R. umbellata in Deliblato Sands, Serbia; ( B ) inflorescence; ( C ) seeds; ( D ) in vitro germination of immature embryos with

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chromosome counts and assess the impact of spontaneous autotetraploidy on pollen viability as determined by in vitro germination and on selected morphological traits including plant height, leaf size, fruit number, fruit dimensions, and weights. Materials and

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either germinated in vitro, or used as a source of cotyledons for in vitro shoot organogenesis. For in vitro germination, the excised embryo was placed on a petri dish (60 × 16 cm) in direct contact with the medium, which consisted of MS basal medium

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-benzylaminopurine (BAP), glutamine, and a high concentration of sucrose, indicating that tissue culture may be a platform for recovering hybrid lilacs. Even if in vitro germination fails, callus developed from the hybrid tissue may provide another source for

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germination and cultured in different in vitro conditions. In vitro culture conditions and measured variables. The Santana-Buzzy et al. (2006) in vitro culture system (coded as MS) was used as the control treatment and modified in the experimental

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-butyric acid (IBA) concentrations on root formation of M. paniculata shoots. Conclusions An efficient protocol through direct shoot organogenesis from epicotyls of in vitro-germinated seedlings of M. paniculata has been described here. This study

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10 mL aliquots into test tubes and then autoclaved at 1.06 kg·cm −2 and 121 °C for 15 min. Inoculation and incubation. Nodal explants were harvested from the in vitro germinated seedlings and inoculated on the microshoot regeneration media under

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ultraviolet light using a light microscope (BX51). In vitro germination assays were conducted according to Yi et al. (2003) . Pollen was inoculated in microwell plates using a standard germination medium (0.062% CaNO 3 and 0.024% boric acid) containing 15

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