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) uprights were tagged in the field according to the corresponding bud width sizes studied: large (L; >1.0 mm); medium (M; 0.6–1.0 mm); and small (S; ≤0.5 mm). No large buds were found on fruiting uprights (FL), represented by the “X” symbol. Histological

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, and cucumber seedlings were used as the scion. Histological aspects, antioxidant enzyme activities, phenylpropanoid contents, and chlorophyll (Chl) fluorescence in the early developmental stages of grafted or nongrafted seedlings were measured to

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regeneration. Medium containing half-strength MS supplemented with 2 μM BA and 0.8 μM NAA after 8 weeks of culture. Historesin sections and SEM. Histological studies showed that morphogenesis gradually changed after the initiation of the culture in 1/2 MS

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the average number of pollen grains per anther ( Chen et al., 2004 ). Histological analysis. Both the wild-type and the mutant flower buds were fixed overnight in 5 formalin : 5 acetic acid : 90 alcohol [FAA (by volume)] at 4 °C. Fixed flower

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having an off year, removed fruit from trees, and/or collected apices in expected floral positions away from developing fruit whenever possible. Collections for histological analyses were performed on 25 Jul, 28 Aug, 5 Oct, and 8 Nov 2018, and 26 Feb 2019

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, 32, 42, 52, 62, 72, 87, 102, 117, 137, 157, 177, and 210 DAA for measurement of fresh fruit weight, histological examination of fruit, and GA analysis. Immediately after collection, the samples were frozen in liquid N 2 and kept in sealed plastic

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the culture period. After 16 weeks of culture, PLB induction rate, necrotic explant rate, and the number of PLBs per explant were recorded. Cultures were examined and photographed with a stereozoom microscope (SZH; Olympus, Tokyo, Japan). Histology of

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. So, little research has been conducted on the histological visualization and localization on triploid pollen morphological features or their developmental process after the irregular distribution of chromosomes at meiosis. There is no palynological

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Materials 3. Histology. A small cut was made through testas of ‘Creole’ ( a ) seeds and seeds grown on F 2 plants of 55-567 × L578. Seeds were placed between moist pieces of filter paper. When the testas were thoroughly moistened, they were slipped

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Abstract

The standard method of preparing plant material by hand for histological study takes several days (1,2). A procedure is described herein which reduces processing time to 17 hours or less and provides tissues which are completely dehydrated and infiltrated. Up to 100 samples can be prepared within this time period. The method requires a minimum expenditure of time without sacrificing cytological detail.

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