Abstract
Red stem, found in PI 157083, is controlled by a single recessive gene, r. Red pigment appeared in vascular traces of hypocotyls about 2 weeks after planting. Seed coat color of red-stemmed plants was reddish or tan, in contrast to white or yellow seed coats of green-stemmed plants. Pale, a spontaneous mutant in a second backcross hybrid from ‘Campo’ × PI 180280, is controlled by a single partially dominant gene, Pa, which acts as a recessive lethal: Pa/Pa plants die; Pa/+ are pale; and +/+ are normal. Pale did not affect expression of red stem. Testcross segregations fit the expected ratio for independent assortment of the 2 loci.
Wild Anagallis monelli has blue or orange flowers. Hybrids with red flowers were developed at the Univ. of New Hampshire. Orange is due to pelargonidin, but delphinidin and malvidin can also be present; red is due to delphinidin and malvidin; and blue is due to malvidin only. In this study, blue and orange wild diploid accessions were used to develop four F2 populations (n = 46 to 81). In three populations, segregation ratios supported a previously proposed three-gene model for flower color in this species (P> 0.01). In the fourth population, white flower color was obtained in addition to blue, orange, and red. Molecular studies of genes in the anthocyanin pathway using a candidate gene approach are in progress. In a separate F2 population, blue, violet, lilac, and red flower colors were obtained. One hybrid per color was studied on three replicate plants. Cells with vacuoles containing anthocyanins in upper and lower petal epidermis peels were counted in five flowers per clone using light microscopy (M = 200×). Blue and red hybrids had mostly blue and red cells, respectively, on both surfaces. Lilac and violet hybrids included cells that were blue and intermediate (containing both red and blue) on both surfaces, and also had red cells on the lower epidermis only. Violet hybrids had more blue cells on the upper epidermis than the lilac hybrids. Anthocyanins were determined by HPLC for each petal epidermis in the four flower colors. The blue hybrid had only malvidin in both upper and lower epidermis, and the red hybrid had mainly delphinidin in both surfaces. Lilac and violet hybrids had small amounts (2% and 2.5%, respectively) of delphinidin on upper surfaces, while lower surfaces had 25% to 33% delphinidin.
Abstract
I am pleased to represent the seed industry in this symposium. However, I would like to qualify my remarks by pointing out that my comments will represent my own personal opinions and to emphasize that my experiences have been entirely in the field of plant breeding in vegetable crops. However, many of the points on which I touch should apply equally as well to breeding and variety work in fruits and ornamentals. I would also like to emphasize that by representing the seed industry our role in this discussion is sort of a middleman's role. The seed industry stands between this tax supported institution and the ultimate consumer, whether it be a home gardener, a roadstand operator, a large truck farmer, or a huge processing industry. This point is very important and should be kept clearly in mind as it has important ramifications in the relationships between tax supported institutions, ASHS and the seed companies. We would like to feel that we are members of both teams and act as sort of a buffer between them. In many cases our personnel are on a par in training and experience with those in public research institutions. Thus I think that some of the remarks I make will be much kinder to members of the Society and the Society itself than other groups may make.
Abstract
The inheritance of resistance to Fusarium solani f. phaseoli Kend. & Sny.) in Phaseolus vulgaris lines P. I. 203958 (N203), and 2114-12 which derives its resistance from P. coccineus, was studied under greenhouse and field conditions. It was concluded that: N203 and 2114-12 respectively possess 4 and 5-6 genes for resistance under the greenhouse-test conditions used; 4 of the 2114-12 genes for resistance are the same as the N203 genes; gene action is mostly additive but partial dominance of resistance appears in 9-13-week-old field-tested plants. Broad sense heritability was estimated as 62-64% under greenhouse conditions and as 22% and 79%, respectively, in 5 and 9-13-week-old field tested plants. The additive variance under greenhouse conditions was estimated as 72% and 40% respectively for resistance from 2114-12 and N203.
Abstract
Resistance to T. basicola was found in Phaseolus vulgaris lines P.I.203958 (N203) and 2114-12. To determine the inheritance of resistance, these 2 lines were crossed with each other and with the susceptible cv. Redkote. Greenhouse tests were conducted on parental, F1, F2, and backcross progenies of each of the 3 crosses, and on F3 progenies of crosses ‘Redkote’ × 2114-12 and ‘Redkote’ × N203. The data indicate that N203 and 2114-12 possess the same genes for resistance, that resistance is partially recessive, and that resistance is controlled by approximately 3 genes. Broad sense heritability was estimated as 59% and the additive variance as 39%.
Abstract
A crack resistant tomato strain,‘Oregon State University 251’ (OSU 251) with fleshy calyx, was crossed with non-fleshy calyx lines to produce F2 segregations of 1 fleshy : 2 intermediate : 1 non-fleshy and backcross ratios of 1 : 1. Short sepal length, wide lobe width, and thick calyx are pleiotropic effects of the fleshy calyx gene, fl. Comparative anatomy of calyx and pedicel is described. The fleshy pedicel of ‘OSU 251’ had the widest cortex, vascular bundle and pith areas, but the areas occupied by the vascular bundle and pith when expressed on a percentage basis were smaller than those of the other parental lines and F1’s. Fruits of segregates with fleshy calyx were significantly more crack resistant than those with non-fleshy calyx. Crack resistance in ‘OSU 251’ is thought not to be a pleiotropic effect of fl, but the fleshy calyx morphology is considered to be of value in selection of plants resistant to fruit cracking.
Iron Deficiency Chlorosis (FeDC) is a problem in cowpea because it affects the ability of the plant to produce chlorophyll. Earlier studies indicated that FeDC was conditioned by a single gene. Pinkeye Purple Hull (PEPH), a susceptible variety, and Texas Pinkeye Purple Hull (TXPE), a resistant variety, were crossed and allowed to self for one generation. The F1s were backcrossed to the parents. SPAD readings were taken on each population. SPAD measures the transmission of light through the leaves at a wavelength where chlorophyll absorbs and a wavelength where it does not. The SPAD reading is calculated based on a ratio of these two numbers. Thus, the SPAD value is unitless and is an indication of the relative amount of chlorophyll present in the leaf. Chlorophyll was extracted from leaves, and regressed on the SPAD readings from the same leaves. An R 2 of .9102 was obtained as well as a regression equation of y = 12.8x + 54.5. Thus, a SPAD value of 1 corresponds with a chlorophyll content of ≈67.3 μg chlorophyll/gfw. The data was analyzed using a bootstrap method, and indicated that FeDC is not controlled by a single gene. A P-value of .0004 showed a highly significant difference between the expected and observed segregation ratios in the F2 plants. Narrow sense heritibility (Mather) was estimated at 0.3.
Progeny of 158 F5 × F5 crosses of Antirrhinum majus (snapdragon) selected within and among cut flower postharvest longevity (PHL) categories (long = 12.6-16.8 days, middle = 9.3-12.1 days, and short = 4.8-8.9 days) were evaluated for PHL and quality traits. Results were compared with previous studies involving F2 × F2 progeny, and F3, F4, and F5 inbred lines. Heritability of PHL in F5 × F5 progeny (0.77 ± 0.11) agrees with that of inbred lines (0.79 to 0.81) but is higher than in F2 × F2 progeny (0.41). Therefore, selection for increased PHL should progress more rapidly and predictably through application of inbred lines rather than F2 individuals. Significant differences between F5 × F5 progeny PHL categories confirm PHL is heritable with a significant additive component. Heritabilities of quality traits in A. majus are high, suggesting selection for quality traits should progress without difficulty. Phenotypic and genotypic correlations of PHL with quality traits are not consistently significant across PHL studies in A. majus. Discrepancies between studies suggest most traits may not be correlated to PHL or are subject to strong environmental influence.