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, pH 2.4, flow rate: 1.0 mL·min –1 , column temperature: 30 °C, detection wavelength 210 nm. Each sample was injected three times, and the corresponding organic acid or sugar content was calculated using the obtained standard curve. The average value

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followed the procedure of Anderson et al. (1992) . Mung bean leaves (1 g fresh weight) were homogenized in 3 mL of ice-cold acidic extraction buffer [6% (w/v) meta-phosphoric acid (pH 2.8), containing 1 m m EDTA], using a Polytron homogenizer (model PT

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± 0.05 g of homogenized sample was added to a tared 15-mL centrifuge tube and the mass was recorded. Ten milliliters of 50% v/v aqueous ethanol acidified to pH 2 (≈1 mL 12.1 M HCL) was added to the 1-g sample and then mixed once every 5 min manually

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186001344; Milford, MA) with an isocratic elution using a phosphate buffer (200 m m ; pH 2.4) as the mobile phase. Ascorbic acid quantification was estimated at 244 nm using calibration curves made with an ascorbic acid standard (Sigma-Aldrich, St. Louis, MO

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mixture (4000 g n × 10 min), the aqueous phase was separated and adjusted to pH 2.8 with 1 m acetic acid and partitioned against 100% ethyl acetate. After being spun down at 16,000 g n for 2 min, the upper organic phase was recovered and completely

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1 and PH2), year, and GDD 45, 60, 90, and 120 DAFB as variables indicated that only GDD 60 DAFB explained significant variation (41%) in diffuse flesh browning. Further ANOVA using GDD 50, 55, 65, and 75 DAFB as variables resulted in 19% of the

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, plants of this species were grown in PIP and AGP and irrigated with fresh and saline water. The following points were studied: 1) substrate temperature and leachate EC and pH; 2) growth and development of the plant and any salt damage; and 3) pore water

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) and concentration of other nutrients, samples were acidified to pH2 using H 2 SO 4 and filtered through a 0.45-µm syringe filter (09-719 F; Thermo Fisher Scientific Inc., Waltham, MA). Samples for determination of soluble PO 4 -P only were not

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Clear (Floralife Inc., Walterboro, SC) at double strength (2× = 32 mL·L −1 water; pH = 2.78; soluble solids = 1.4%). Crystal Clear contains no hormones and did not affect the rate of budbreak in comparison with water but did reduce bacterial growth in

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Authors: , , and

was also suspected that phytotoxicity was associated with the low pH of ABA solutions, which varied from pH = 2.8 for 3200 mg·L −1 to pH = 3.8 for 200 mg·L −1 . However, buffered ABA solutions to neutral pH led to more injury at equal concentration

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