, pH 2.4, flow rate: 1.0 mL·min –1 , column temperature: 30 °C, detection wavelength 210 nm. Each sample was injected three times, and the corresponding organic acid or sugar content was calculated using the obtained standard curve. The average value
followed the procedure of Anderson et al. (1992) . Mung bean leaves (1 g fresh weight) were homogenized in 3 mL of ice-cold acidic extraction buffer [6% (w/v) meta-phosphoric acid (pH 2.8), containing 1 m m EDTA], using a Polytron homogenizer (model PT
± 0.05 g of homogenized sample was added to a tared 15-mL centrifuge tube and the mass was recorded. Ten milliliters of 50% v/v aqueous ethanol acidified to pH 2 (≈1 mL 12.1 M HCL) was added to the 1-g sample and then mixed once every 5 min manually
186001344; Milford, MA) with an isocratic elution using a phosphate buffer (200 m m ; pH 2.4) as the mobile phase. Ascorbic acid quantification was estimated at 244 nm using calibration curves made with an ascorbic acid standard (Sigma-Aldrich, St. Louis, MO
mixture (4000 g n × 10 min), the aqueous phase was separated and adjusted to pH 2.8 with 1 m acetic acid and partitioned against 100% ethyl acetate. After being spun down at 16,000 g n for 2 min, the upper organic phase was recovered and completely
1 and PH2), year, and GDD 45, 60, 90, and 120 DAFB as variables indicated that only GDD 60 DAFB explained significant variation (41%) in diffuse flesh browning. Further ANOVA using GDD 50, 55, 65, and 75 DAFB as variables resulted in 19% of the
, plants of this species were grown in PIP and AGP and irrigated with fresh and saline water. The following points were studied: 1) substrate temperature and leachate EC and pH; 2) growth and development of the plant and any salt damage; and 3) pore water
) and concentration of other nutrients, samples were acidified to pH ≈2 using H 2 SO 4 and filtered through a 0.45-µm syringe filter (09-719 F; Thermo Fisher Scientific Inc., Waltham, MA). Samples for determination of soluble PO 4 -P only were not
Clear (Floralife Inc., Walterboro, SC) at double strength (2× = 32 mL·L −1 water; pH = 2.78; soluble solids = 1.4%). Crystal Clear contains no hormones and did not affect the rate of budbreak in comparison with water but did reduce bacterial growth in
was also suspected that phytotoxicity was associated with the low pH of ABA solutions, which varied from pH = 2.8 for 3200 mg·L −1 to pH = 3.8 for 200 mg·L −1 . However, buffered ABA solutions to neutral pH led to more injury at equal concentration