isolates. The number of single isolate groups (11) is high. Hyun and Park (1996) from Korea identified four major VCGs, with only one of these being a single isolate group. Phylogenetic analysis based on EF-1α sequences showed variability among isolates
Michelle L. Paynter, Elizabeth Czislowski, Mark E. Herrington and Elizabeth A.B. Aitken
Irene Kadzere, Chris B. Watkins, Ian A. Merwin, Festus K. Akinnifesi, John D. K. Saka and Jarret Mhango
The full commercial potential of wild loquat [Uapaca kirkiana (Muell. Arg.)], a fruit that is widely used for food and income in parts of Africa, is restricted by its short shelf life and variability in postharvest quality. We have evaluated within and among tree variability in fruit size and color at harvest, and changes of color, soluble solids concentrations (SSC) and pulp deterioration during storage, of fruit harvested during the maturation period. The relationships between fruit shape, size, seed number and SSC of fruit harvested at the ripe stage of maturity was also assessed. Size and color of fruit within and among trees at harvest varied greatly within the same location on the same harvest date. The a* values (redness) were more variable than for other color attributes, reflecting a range of fruit colors from greenish to brown. During a 6 day storage period, fruit color lightness and yellowness decreased, while redness increased, and variation in color attributes decreased. Although fruit color intensified during storage, the SSC of fruit after ripening was linked more with fruit color at harvest, with mean concentrations ranging from 6.7% to 13.8% among trees. When fruit were harvested four weeks later and categorized by color at harvest, SSC varied from 11.8% in greenish-yellow fruit to 14.5% in browner fruit. Pulp deterioration of stored fruit harvested unripe was observed by 6 days. The SSC of fruit harvested when ripe was not significantly correlated with shape, size or seed number. These observations have important implications for germplasm selection and collection of U. kirkiana for domestication purposes. Timing of harvest and/or postharvest sorting of fruit is likely to reduce variability in SSC during the postharvest period.
K.H.S. Peiris, G.G. Dull, R.G. Leffler and S.J. Kays
Spatial variation in soluble solids content (SSC) of fruits of apple (Malus ×domestica Borkh. cv. Red Delicious), cantaloupe (Cucumis melo L. Cantaloupensis group), grapefruit (Citrus paradisi Macf. cv. Indian River Ruby Red), honeydew melon (Cucumis melo L. Inodorus group), mango (Mangifera indica L. cv. Hayden), orange (Citrus sinensis L. Osbeck. cv. Valencia), peach (Prunus persica L. Batsch. cv. Windblow), pineapple (Ananas comosus L. Merr. cv. Kew) and tomato (Lycopersicon esculentum Mill.), and of bulbs of onion (Allium cepa L. Cepa group) and in dry-matter content (DMC) of potato (Solanum tuberosum L. cv. Russet Burbank) tubers was measured along three directional orientations (i.e., proximal to distal, circumferentially midway along the proximal to distal axis, and radially from the center of the interior to the outer surface). The pattern and magnitude of constituent variation depended on the type of product and the direction of measurement. Radial and proximal to distal variation was greater than circumferential variation in all the products tested. Honeydew had the highest radial variation with a SSC difference of 6.0 % and a cv of 22.8%, while tomato displayed lower radial variation with a cv of 1.0%. Pineapple had a proximal to distal SSC difference of 4.6% with a cv of 13.8%, while the difference in tomato was 0.6% with a cv of 5.1%. Circumferential variation of SSC in all products tested was <2% with cv ranging from 1.1% to 3.8%. The results confirm that considerable constituent variability exists within individual fruit and vegetable organs. This variability may affect the accuracy of calibration equations and their prediction capability. Therefore, within-unit constituent variability should be meticulously assessed when an NIR spectrometric method is being developed for the nondestructive quality evaluation and sorting of a product.
Santiago Pereira-Lorenzo, Ana M. Ramos-Cabrer, Belén Díaz-Hernández, Javier Ascasíbar-Errasti, Federico Sau and Marta Ciordia-Ara
Chestnut (Castanea sativa Mill.) is an important crop in Spain. This inventory of chestnut cultivars complements previous studies. We have located 152 chestnut cultivars in 131 municipalities covering 108.6 ha, with 72 new cultivars in addition to the 80 previously found. Fewer than 50% of these cultivars are extensively cultivated. Chestnuts in Spain are grown from sea level to 1100 m, but are more frequent between 200 and 800 m on northern-facing slopes. Most of the chestnuts are harvested from 25 Oct. to 10 Nov.
Paul R. Hepler and David E. Yarborough
One hundred lowbush blueberry (Vaccinium angustifolium Ait.) clones were randomly sampled from a commercial field to estimate potential productivity. Yield data exhibited a normal distribution ranging from 300 to 17,000 kg·ha-1 with a mean of 7726 kg·ha-1. Commercial use of selected clones or improved cultivars through new plantings, interplanting into existing clones, or replacement of low-yielding clones in native stands and increasing the intensity of field management would increase the yielding potential of native lowbush blueberry fields.
Patrick P. Moore
Cultivated raspberries may include North American red raspberry (Rubus idaeus strigosus Michx), European red raspberry (R. idaeus vulgatus Arrhen.) or black raspberry (R. occidentalis in their pedigrees. Twenty-one raspberry clones were investigated using chloroplast restriction fragment length polymorphisms to determine the cytoplasm type and the amount of cytoplasmic diversity among these selected clones. The raspberry clones were selected representing North American red raspberry, European red raspberry, black raspberry and cultivars with divergent maternal lineages. Total cellular DNA was probed with two 32P-labelled fragments of tomato chloroplast DNA. Probe-restriction enzyme combinations were selected which discriminated between representatives of the two red raspberry subspecies. Raspberry clones were grouped according to the chloroplast restriction fragment patterns. The composition of the groups was compared with their pedigrees.
Himayat H. Naqvi, Mitsuo Matsumura and Irwin P. Ting
Clarissa J. Maroon-Lango, Mary Ann Guaragna, Ramon Jordan, John Hammond, Murali Bandla, Steve Marquardt and John R. Stommel
Pepino mosaic virus (PepMV) was first found in pepino (Solanum muricatum) growing in coastal Peru in 1974 and described in 1980; it reappeared in protected tomato (Lycopersiconesculentum) in the Netherlands in 1999. Since then, it has been reported to occur in tomato in several countries including Austria, Belgium, Canada, France, Germany, Italy, Peru, Spain and the Canary Islands, the United Kingdom, and in 11 states within the United States. Three strains of PepMV found in the United States have been cloned and sequenced. Full-length genomic sequences were obtained for two strains, PepMV-US1 and PepMV-US2, from co-infected tomato plant samples from Arizona. The 3'-end sequence of PepMV-US3 came from infected tomato fruits from Maryland. The genome organization, motifs and domains typical of the genus Potexvirus, and of other PepMV isolates, were found in full-length sequences of both US1 and US2 isolates. Direct comparison of US1 and US2 at the nucleotide level revealed an 86.3% identity; whereas, when individually compared to the French and Spanish isolates, which share ∼99% identity at the nucleotide level, US1 and US2 had 82% and 79% identities to each, respectively. Pair-wise gene-for-gene comparisons between United States and European isolates revealed a similar trend. While unique, US1 is more closely related to the previously reported European isolates than is US2. The CP of US3 is nearly identical to the European isolates at the amino acid level. None of 18 tomato germplasm accessions or 10 cultivars were resistant to mechanical inoculation with US3; in contrast, no infection was detected in nine pepper cultivars or four germplasm accessions. Plants grown from seeds of infected tomato fruits did not test positive for PepMV.
Robert D. Belding, Sylvia M. Blankenship, Eric Young and Ross B. Leidy
Variation in amount and composition of epicuticular wax among several apple (Malus ×domestica Borkh.) cultivars was characterized by gas chromatography, thin-layer chromatography, and gas chromatography-mass spectroscopy. Across cultivars, wax mass ranged from 366 to 1038 μg·cm-2. Wax mass decreased during the 30 days before harvest. Ursolic acid accounted for 32% to 70% of the hydrocarbons that make up the epicuticular wax. Alkanes, predominantly 29-carbon nonacosane, comprised 16.6% to 49%. Primary alcohols of the hydrocarbons ranged from 0% to 14.6% of the epicuticular wax. Secondary alcohols of the hydrocarbons were the most cultivar specific, making up 20.4% of the epicuticular wax in `Delicious' and only 1.9% `Golden Delicious' strains. Aldehydes and ketones of the hydrocarbons represented a small amount of total wax, ranging from 0% and 6.0%. Percentage of primary alcohol in the epicuticular wax increased as fruit developed. Other components showed no distinct trends with fruit development. Examination of the ultrastructure of cuticular wax using scanning electron microscopy revealed structural differences among cultivars.
E.A. Guertal and C.B. Elkins
Photosynthetically active radiation (PAR) was measured at two times of day (8:00 am and noon Central Standard Time) in a 915 × 915-cm area of a 1006 × 915-cm gable roof greenhouse. PAR measurements were taken across a grid at 40-cm intervals, a total of 529 data points. Spatial variation of PAR in the greenhouse was evaluated through contour plots and the geostatistical technique of semivariogram construction. Semivariograms provide a visual guide to the degree of spatial correlation of a variable, allowing a quantification of the distance at which variables cease to be spatially correlated (the range) Measured PAR contained distinct zones of lowered values, a function of overhead greenhouse structures, wall-hung electrical boxes, and tall plants in adjacent greenhouses. Although the amount of PAR changed over time, zones of high and low PAR remained relatively constant, except at the sides of the greenhouse. Constructed semivariograms revealed that PAR contained strong spatial correlation (up to a 350-cm separation) as measured in the north-south direction, moving parallel to greenhouse bench placement. When PAR measurements perpendicular to benches (east-west) were used in directional semivariograms PAR was found to be completely random, plotting as a horizontal line called a nugget effect. Thus, plants placed perpendicular to the greenhouse benches (east-west) would not be affected by the spatial correlation of PAR.