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Benjamin Ewa Ubi, Daisuke Sakamoto, Yusuke Ban, Takehiko Shimada, Akiko Ito, Ikuko Nakajima, Yoshihiro Takemura, Fumio Tamura, Toshihiro Saito and Takaya Moriguchi

–1 and PpMADS13–2 cDNA sequences were used to search homologous sequences via tBLASTx in National Center for Biotechnology Information (NCBI). Multiple alignments of the predicted full-length amino acid sequences were performed between ‘Kosui’ DAM

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Martin P.N. Gent

in the metabolite profiles of hydroponic spinach leaves were measured for different concentrations of nitrate in solution. Soluble amino acids were 5% to 12% of total nitrogen in spinach with free glutamine predominant ( Eppendorfer and Bille, 1996

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Yansheng Li, Ming Du, Qiuying Zhang, Guanghua Wang, Jian Jin, Stephen Herbert and Xiaobing Liu

July, August, and September i.e., R1 to R8 stage of soybean at Harbin in 2010 and 2011. Measurement of protein, oil, and free amino acid content in seed. The crude protein of vegetable soybean was determined using the method of combustion N analysis by

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Jiyu Zhang, Min Wang, Zhenghai Mo, Gang Wang and Zhongren Guo

μg total RNA with an oligo(dT) 18 adaptor primer using PrimeScript RTase (TaKaRa, Japan). We have obtained eleven highly conserved MADS domain amino acid motifs from pecan previously. In this study, we cloned the full-length cDNA of the fragment 1

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Karen R. Harris, W. Patrick Wechter and Amnon Levi

Information. Those sequences with similarity to resistance genes were assembled into contigs. A representative from each contig was translated using ExPASy ( Gasteiger et al., 2003 ) and these amino acid sequences were aligned using AliBee ( Brodsky et al

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Cai-Hong Jia, Ju-Hua Liu, Zhi-Qiang Jin, Qiu-Ju Deng, Jian-Bin Zhang and Bi-Yu Xu

acid oxidation, nitrogen assimilation, amino acid synthesis, plant growth, pollen development, and improving plant tolerance to aluminum ability ( Kochianl et al., 2004 , Ma et al., 2001 , Yao et al., 2011). As a result of its multifaceted activities

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Xiyan Yu, Xiufeng Wang, Jide Fan, Hongmei Tian and Chengchao Zheng

. To isolate SPS cDNA from muskmelon, we first designed a pair of degenerated primers according to the conserved domain of SPS amino acid sequences from other plant species. A 1408-bp cDNA fragment was amplified by RT-PCR. A 1600-bp and a 750-bp cDNA

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Suping Zhou, Roger Sauve and Fur-Chi Chen

). To characterize the primary structure of the gene, the DNA sequence was translated into protein using the ExPaSy translation tool. The deduced polypeptide has a predicted length of 374 amino acids, molecular mass of 40.9 kDa, and a pI of 5.70. The

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Yi Zhang, Xiao-Hui Hu, Yu Shi, Zhi-Rong Zou, Fei Yan, Yan-Yan Zhao, Hao Zhang and Jiu-Zhou Zhao

incorporated into organic compounds such as amino acids, proteins, and other cellular compositions necessary for plant growth and development ( Zhang et al., 2006 ). This process is mainly carried out by the synergistic action of GDH, GS, GOGAT, GOT, and GPT

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Shuai-Ping Gao, Kang-Di Hu, Lan-Ying Hu, Yan-Hong Li, Yi Han, Hui-Li Wang, Kai Lv, Yong-Sheng Liu and Hua Zhang

Commission 1.6.4.2) was determined by the method described by Knörzer et al. (1996) . Detection of reducing sugars, ascorbate, soluble protein, and free amino acids. Fruit samples (5.00 ± 0.05 g) were ground in 5 mL of phosphate buffer (pH 7.0, 200 mmol·L −1