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Chun-yan Yang, Yuan Huang and Chunlin Long

.8183 and 0.0417 to 1, respectively. Among 17 microsatellite markers, 11 loci showed significant deviation from Hardy-Weinberg equilibrium ( P < 0.01), probably due to deficiency of heterozygote or the limitation of sample size. Tests for linkage

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Brooke C. Colburn, Shawn A. Mehlenbacher, Vidyasagar R. Sathuvalli and David C. Smith

. To assign EFB resistance from the new sources to a LG, sets of 32 seedlings plus the two parents of progenies 01035, 05024, and 06027 were amplified with microsatellite primer pairs. A set of 24 microsatellite markers that had been previously mapped

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Kwanjai Pipatchartlearnwong, Akarapong Swatdipong, Supachai Vuttipongchaikij and Somsak Apisitwanich

conservation plans. New microsatellite markers require a high developing cost. Nonetheless, many microsatellite markers have been shown to be transferable across plant species and genera, providing an economical way for marker development ( Karaca et al., 2013

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Theodore J. Kisha and Christopher S. Cramer

. Additionally, analysis of worldwide genetic diversity can identify areas suited for the establishment of in situ conservation sites ( Greene et al., 2008 ). Microsatellite markers, or simple sequence repeats [SSRs ( Oliveira et al., 2006 ; Tautz and Renz, 1984

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Zhan Shu, Xue Zhang, Dianqiong Yu, Sijia Xue and Hua Wang

( Hoban et al., 2009 ; Pollegioni et al., 2009 ; Ross-Davis et al., 2008 ; Wang et al., 2015 ). To investigate the possibility of genetic invasion, we used 12 microsatellite markers to identify the hybrids between J. regia and J . cathayensis in

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Patricio A. Brevis, Nahla V. Bassil, James R. Ballington and James F. Hancock

objectives of this study were: 1) to report the expected genetic contribution of the Vaccinium species that constituted the current genetic base of cultivated SHB; 2) to establish the usefulness of microsatellite markers in assessing genetic relationships

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Chunxian Chen and William R. Okie

et al., 2007 ; Sitther et al., 2012 ; Testolin et al., 2000 ). Likewise, there are many reports on the development and use of chloroplast microsatellite markers in plants ( Brundu et al., 2008 ; Gavrilenko et al., 2013 ; Molina-Cano et al., 2005

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O. Gulsen and M.L. Roose

Inter-simple sequence repeats (ISSR), simple sequence repeats (SSR) and isozymes were used to measure genetic diversity and phylogenetic relationships among 95 Citrus L. accessions including 57 lemons [C. limon (L.) Burm. f.], related taxa, and three proposed ancestral species, C. maxima (Burm.) Merrill (pummelo), C. medica L. (citron), and C. reticulata Blanco (mandarin). The ancestry of lemons and several other suspected hybrids was also studied. Five isozyme and five SSR loci revealed relatively little variation among most lemons, but a high level of variation among the relatively distant Citrus taxa. Eight ISSR primers amplified a total of 103 polymorphic fragments among the 83 accessions. Similarity matrices were calculated and phylogenetic trees derived using unweighted pair-group method, arithmetic average cluster analysis. All lemons, rough lemons, and sweet lemons, as well as some other suspected hybrids, clustered with citrons. Most lemons (68%) had nearly identical marker phenotypes, suggesting they originated from a single clonal parent via a series of mutations. Citrons contributed the largest part of the lemon genome and a major part of the genomes of rough lemons, sweet lemons, and sweet limes. Bands that characterize C. reticulata and C. maxima were detected in lemons, suggesting that these taxa also contributed to the pedigree of lemon.

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Timothy A. Rinehart, Brian E. Scheffler and Sandra M. Reed

Recent evidence suggests a close genetic relationship between Hydrangea macrophylla (Thunb.) Ser. and D. febrifuga Lour., which supports previous morphological and DNA sequence data. This relationship was confirmed by the production of fertile intergeneric hybrids. We characterize the genetic diversity of available D. febrifuga plants, both cultivars and wild-collected taxa, as breeding material to improve H. macrophylla. Relatively high genetic diversity is seen among D. febrifuga, which splits into two main clusters. We also document considerable differences in genome size when compared with previously characterized D. febrifuga. Dichroa versicolor (Fortune) D.R. Hunt plants were also included and data suggest that D. versicolor could be a hybrid between H. macrophylla and D. febrifuga, similar to the intergeneric hybrids produced by recent breeding efforts. Because native H. macrophylla plants do not overlap extensively with D. febrifuga populations, we tested Hydrangea indochinensis Merr. as a possible parent because endemic H. indochinensis populations overlap regions where D. febrifuga and D. versicolor have been collected. However, results suggest that H. indochinensis does not share a genetic background with D. versicolor. Taxonomic revision of Dichroa is warranted, especially because we document several more intergeneric hybrids from self-sown, open-pollinated sources.

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Eder J. Oliveira, Maria Lucia C. Vieira, Antonio Augusto F. Garcia, Carla F. Munhoz, Gabriel R.A. Margarido, Luciano Consoli, Frederico P. Matta, Michel C. Moraes, Maria I. Zucchi and Maria Helena P. Fungaro

in a 1:1 ratio were used, as were 116 biparental loci segregating in a 3:1 ratio. Microsatellite markers. One hundred seven microsatellite primers developed from enriched genomic libraries ( Oliveira et al., 2005 ) were tested. All primer