). The suspension was filtered through a 50-μm nylon mesh screen to remove plant debris. Nuclei were stained using 1.6 mL staining buffer containing 4′, 6-diamidino-2-phenylindole (CyStain ultraviolet Precise P; Partec). Stained nuclei were analyzed with
up larger soil aggregates, and an Austrian-style hay rake to remove excess debris and spread compost over the soil surface. Compost is left on the surface to facilitate water absorption, to prevent crusting during seed germination, and to initially
presented at the workshop included: 1) the U.S. Department of the Interior, National Park Service funded the development of educational resources to discourage home debris burning and encourage composting; 2) funding from the EMG state association was used
cold tap water, transferred with 70% ethanol to petri dishes, and fauna therein were counted under a stereoscope. This method resulted in more plant debris in the rinsate and required more time to sort through debris under the stereoscope. Blender
. Colletotrichum acutatum conidia may survive in soil and plant debris under dry conditions for up to 12 months, but conidia and sclerotia die rapidly under moist conditions, i.e., soil moisture ≥12% ( Norman and Strandberg, 1997 ). ANTHRACNOSE CULTURAL
washed off debris) was decanted and a second wash of 2500 ppm Trichlor was applied to seed with agitation for 10 to 15 min and subsequently decanted. For seed that were chemically mutagenized, the initial supernatant was decanted and a solution of 0.1 M
for 10 min to pellet large debris. One milliliter of supernatant was transferred to a microcentrifuge tube and centrifuged for 5 min at 13,000 g n to pellet smaller debris. Juice was filtered through 0.20-μm nylon membranes (Millipore, Bedford, MA
content and sands are more prone to surface drying ( Müller and Deurer, 2011 ). On sand soils, SWR is universally attributed to hydrophobic organic matter coating on the sand particle surfaces and hydrophobic organic particulate matter from plant debris or
experimental unit (i.e., with yield from the two sample trees in each plot pooled) using a pecan harvester (Savage Model 8042, Madill, OK). To account for varying levels of debris and leaf litter in the piles of nuts after they had been mostly cleaned by
by lettuce plant debris in soil or leaves of symptomless weed species after the summer and winter fallow periods in California ( Barak et al., 2001 ). Xcv has also been demonstrated to be spread rapidly by overhead irrigation in greenhouse from only