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Radius of gyration (size), intrinsic viscosity, molecular weight, percentage of galacturonate, and percentage of neutral sugars were measured for chelate-soluble (CSP) and alkaline-soluble (ASP) pectins extracted from the cell walls of melting flesh (MF) and nonmelting flesh (NMF) peach [Prunus persica (L.) Batsch]. Weight percentage of cell walls, pectin content, and firmness were measured also. Peaches were extracted at 20, 21, and 22 weeks after flowering (WAF) and after various lengths of shelf storage at 25 ± 2C for the peaches picked at 21 WAF. Weight percentage of cell walls and firmness decreased markedly between the 21st and 22nd WAF; and between the 3rd and 6th day of storage for MF peaches as compared to NMF peaches. During these same periods, there were marked drops in the pectin content and the uronide content for MF as compared to NMF peaches. Size and intrinsic viscosity dropped markedly for CSP of MF peaches in comparison with NMF peaches during these same periods, whereas the molecular weight of CSP and ASP increased in MF peaches over that measured for NMF peaches. These results suggested that α -D-galacturonase (E.C. 3.2.1.15) was involved in softening only in the latter stages of ripening MF peaches. Further, cell wall polymers containing long thin pectin aggregates were destroyed, whereas cell wall polymers containing short thick pectin aggregates remained.
Pectin metabolism was analyzed in tabasco pepper (Capsicum frutescens L.) to determine the metabolic process associated with the ease of fruit detachment from the calyx. The ease of fruit detachment (deciduous fruit) is a desirable trait in peppers that facilitates mechanical harvest. Two genotypes that differ in the fruit detachment force were used: `Easy Pick' (EZ), which requires a low force, and `Hard Pick' (HP), which requires higher force. Pectin dissolution in water from fresh-ripe EZ tissue was 20 times higher than from HP tissue. EDTA-soluble uronide from inactivated EZ cell wall, however, was only 1.8 times higher. Pectin dissolution was inversely correlated to the fruit detachment force and followed a sigmoidal curve during fruit ripening. Size-exclusion chromatography of EDTA-soluble polyuronides indicated that pectin was degraded in ripe fruit tissue from both genotypes. The degree of depolymerization, however, was more extensive in EZ fruit. Consequently, the ease of fruit detachment was attributed to pectin ultra-degradation. Total pectin content in dry tissue and ethanol/acetone-extracted cell wall was similar in both genotypes. Pectin content in dry tissue was maintained throughout ripening, while extracted cell wall pectin increased slightly. In contrast, the degree of pectin esterification of extracted cell wall decreased only in ripe EZ fruit. These results suggest that pectin de-esterification may have a role in the enhanced pectin depolymerization and consequently in the ease of fruit detachment of the EZ genotype.
. hupehensis leaves, waterlogging altered the transcription factors (TFs) and genes involved in carbohydrate and energy metabolism; polysaccharide, cell wall, and cytoskeleton metabolism; antioxidation and detoxification; lipid, protein, and amino acid
The tensile properties of european pear (Pyrus communis L. `Beurre Bosc') and asian pear (Pyrus pyrifolia Nakai `Choguro') were examined using a microscope-mounted apparatus that allowed direct observation and recording of cell and tissue changes during testing. To manipulate turgor potential, tissue slices from fruit of different firmness (ripeness) were incubated in sucrose solutions of differing water potential. Solution water potentials were adjusted for individual fruit, and varied between -2.5 and 1 MPa from the water potential of the expressed juice. Fruit firmness declined from 100 to 20 N and from 60 to 25 N during ripening of european and asian pears, respectively. For both european and asian pears the relationship between fruit firmness and tensile strength of tissue soaked in isotonic solutions was sigmoidal, with the major mechanism of tissue failure being cell wall failure and cell fracture at high firmness and intercellular debonding at low firmness. In the intermediate zone, where fruit firmness and tissue tensile strength decreased simultaneously, a mixture of cell wall rupture and intercellular debonding could be observed. Tissue and cell extension at maximum force both declined similarly as fruit softened. Tensile strength of tissue from firm pears (>50 N firmness, >0.8 N tensile strength) decreased by as much as 0.6 N during incubation in solutions that were more concentrated than the cell sap (hypertonic solutions). When similar tissue slices were incubated in solutions that were less concentrated than the cell sap (hypotonic solutions), the tensile strength increased by up to 0.4 N. This is interpreted as stress-hardening of the cell wall in response to an increase in cell turgor. Tensile strength of tissue from soft pears was not affected by osmotic changes, as the mechanism of tissue failure is cell-to-cell debonding rather than cell wall failure.
Abstract
Morphological changes in tannin cells were observed in ‘Fuyu’ [pollination-constant and nonastringent (PCNA)] and ‘Hiratanenashi’ [pollination-variant and astringent (PVA)], two of the four types of Japanese persimmon fruit (Diospyros kaki L.). Pores in the tannin cell walls of ‘Fuyu’ started to occlude on 24 July when cell enlargement had ceased. This occlusion coincided with cessation of tannin accumulation, as determined by soluble tannin content and fresh weight of fruit. The pores were almost completely occluded on 7 Aug. Pore occlusion preceded the coagulation of tannins. In ‘Hiratanenashi’, pores in the tannin cell walls expanded until 7 Aug. When enlargement of tannin cells ceased on 14 Aug., occlusion of the pores in the cell walls was initiated. This event also nearly coincided with cessation of tannin accumulation. The process of occlusion was much slower than in ‘Fuyu’ and was about complete on 16 Oct. Thus, the pores in the tannin cell walls appear to be involved in tannin accumulation.
The activity, isoenzymes, and histochemical location of peroxidase were studied in developing highbush blueberries (Vaccinium corymbosum L.). Total peroxidase activity increased during development when expressed on a fresh-weight basis, reaching a maximum in red berries and then declining. When peroxidase activity was expressed per berry it did not decline after the red-berry stage. Most of the enzyme was ionically bound to cell walls throughout development, with the number of isoenzymes increasing with maturity. Histochemical localization of peroxidase showed that enzyme activity spread throughout the berry during development.
A nonenzymatic technique using dilute salt solutions effected rapid release of viable protoplasts from mature bean (Phaseolus vulgaris L.) pollen. Protoplasm release started within 30 sec and was completed within 5 min in solutions of 0.02 to 0.06 m NaCl, or KCl, pH 6 to 9. The degree of release could be altered by changing the concentrations and ratios of CaCl2 and H3BO3 and by adding sucrose to either solidified or aqueous salt media. The surface of nonenzymatically released protoplasts was partially digested by short-time exposure to a mixture of cell wall-degrading enzymes and then examined by scanning electron microscope.
Lignin is one of the main components of plant cell walls. It is a phenol polymer composed of coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol subunits. Lignin can enhance the strength of cell walls to maintain a plant’s structure and
, only A cell walls turned bright red—an indication of the presence of PAs (data not shown). We also examined sections of G141 ( A ) and ‘Perfection’ ( a ) testas (kindly provided by R. Provvidenti, who had stained them with safranin). The
During three consecutive years of field experiments, three crop-covering treatments [noncovered (C), perforated polyethylene (PO, 500 holes/m2), and a nonwoven polypropylene (AO) sheet] were used to create different environmental conditions for growth of `Nagaoka 50' chinese cabbage [Brassica rapa L. (Pekinensis Group)]. The PO and AO treatments reduced solar irradiance and increased air and root temperatures compared to C plants. Plants were sampled five times each year from transplanting to harvest, and fresh and dry weights, yield at harvest, leaf pH, citric and ascorbic acid concentrations, and cell-wall fractions were determined. The PO floating row cover was the most beneficial for yield and chemical composition of chinese cabbage of the early spring crop in southern Spain, where environmental conditions during an unfavorable season can injure sensitive crops.