dihydrogen phosphate and 4.0 m m triethylamine adjusted to pH 2.75 with 85% orthophosphoric acid. The flow rate was 1.0 mL·min –1 and the total chromatographic run time was 14 min. The sample injection volume was 50 μL. To improve the detector sensitivity
-18e resin). Elution was performed in a stepwise gradient with a water (pH 2.6 adjusted with H 3 PO 4 )/acetonitrile (ACN) volumetric ratio of 0 min, 7% ACN; 0 to 20 min, 20% ACN; 20 to 28 min, 23% ACN; 28 to 40 min, 27%, ACN; 40 to 45 min, 29%, ACN
chelate-associated Fe solubility is 1) a function of substrate pH; 2) chelating agent stability constants with other cations like Ca, Cu, Mn, and Zn; and 3) relative abundance of these cations ( Boxma, 1981 ; de Kreij, 1998 ; Lindsay and Norvell, 1969
of ethanol 70% and 30 mL of HCl 0.1%, pH 2.0) for 2 min in a blender, then placed in a beaker covered with parafilm and aluminum foil, and extracted in darkness for 12 h at 4 °C. Afterward, a filtration was made, transferring the content to a 250-mL
samples were collected 39, 74, 94, and 138 DAPF in 2014; and 30, 63, 93, and 122 DAPF in 2015. Soil P was extracted by Mehlich-3 [solution pH, 2.5; soil:solution ratio, 1:10 ( Mehlich, 1984 )], AB-DTPA [solution pH, 7.6; soil:solution ratio, 1
(pH 2.2) and the flow rate was 1 mL·min −1 . Statistical analysis. Statistically significant differences ( P ≤ 0.05) were determined for the microbial counts, gas concentration, and visual and physicochemical evaluation data based on an analysis of
evaporator and the aqueous phase was adjusted to pH 2.8 with 1% hydrochloric acid (Merck) and extracted three times with ethyl acetate (Merck). It was evaporated to dryness, dissolved in 1 mL of HPLC methanol, and used for of ABA and GA 3 quantification at
) at pH 2.4 ± 0.01 was used for vitamin C analysis. Two milliliters of juice were mixed with 2 mL of 2.4% (w/v) metaphosphoric acid and centrifuged at 6900 g n for 5 min at 5 °C. An aliquot of the centrifuged sample (0.5 mL) was then transferred to a
rootstock growth through increasing soil pH; 2) biochar can improve apple rootstock growth through increasing soil microbial biomass and hence increase nutrient mineralization and availability; and 3) biochar can increase apple rootstock growth in
cultivars of blueberry and found that the maximum vegetative growth occurred at pH 4.0 and 4.5, but that there was no significant growth at pH 2.5 and 3.0. However, Schmid et al. (2009) used sulfur to acidify pine sawdust to a pH range of 3.8 and 4.2, and