concentrations. In vitro shoot culture stock. Microcutting explants used for in vitro experiments were taken from seedlings obtained from the germination experiments, surface-sterilized by soaking in a 0.5% (v/v) NaOCl solution containing one drop of the
, D. 2016 In vitro germination of early ripening sweet cherry varieties ( Prunus avium L.) at different fruit ripening stages Erwerbs-Obstbau 58 2 136 137 doi: 10.1007/s10341-016-0265-y 10.1104/pp.39.4.691 Emershad, R.L. Ramming, D.V. 1994 Effect of
of seven South American Ilex species and comparing different methodologies for in vitro germination of fresh and cryostored seeds, including I. paraguariensis (the source of raw material for the production of maté, the most popular beverage in the
with this frequent watering schedule. This study aimed to establish a fast and efficient protocol for in vitro seed germination using different nutrient media and PGR combinations, and to optimize seedling acclimatization protocols using different
many crops to overcome seed dormancy and shorten seed germination time ( Sharma et al., 1996 ). In vitro germination of immature embryos might be helpful to shorten breeding cycles and accelerate the breeding process. In vitro cultures, plant embryos
viability can be defined as the potential of a pollen grain to germinate, grow, and effect seed set ( Dafni et al., 2005 ). Thus, the concept of pollen viability can be applied to infer the fertility of a given plant. In vitro pollen germination is a common
of storage and temperature on C. nepeta seed germination ability, and 2) the effect of various plant growth regulators and agar concentrations on in vitro shoot proliferation, elimination of hyperhydricity problems, and microshoot rooting. Materials
shoot tips and nodes of D. ombet seedlings onto MS medium without plant growth regulators (PGRs) for 6 months produced sufficient stock of shoots for further experiments. Fig. 1. In vitro seed germination and shoot proliferation of Dracaena ombet
refine the standard seed germination protocol, in vitro seed germination methodology, and vegetative propagation techniques, including in vitro multiplication of cloned plantlets, to facilitate ex situ conservation and development of a new methodology for
) with gold. Samples were examined using a SEM (JSM 5800; JEOL, Tokyo, Japan) at 15 kV. In vitro pollen germination. In vitro germination assays were conducted according to Yi et al. (2003) . Freshly collected pollen was inoculated into 96-well plates