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of raffinose was added as an internal standard to each thawed sample that was then neutralized with a 1% sodium hydroxide solution and filtered through a membrane filter (0.45 μm). The filtrate was used for the sugar analyses. Another series of thawed

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-zone salinity altered raffinose oligosaccaride metabolism and transport in Coleus ( Gilbert et al., 1997 ). In salt-stressed celery, mannitol becomes the predominant photosynthetic product ( Everard et al., 1994 ). Sorbitol accumulates to high levels in the

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raffinose/sucrose/glucose kit (product K-RAFGL; Megazyme International Ireland, Bray, Ireland) ( McCleary et al., 2006 ); methods of Landry and Wolyn (2011) were followed with one modification. The rhizome and storage root samples were incubated with 5 mL

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raffinose was added to each sample as the internal standard. After extraction, the tissue suspension was centrifuged at 4000 g n for 10 min to collect the supernatant. Each sample was again extracted twice, and the three supernatants were pooled together

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, M.A. 1999 Water deficit effects on raffinose family oligosachharide metabolism in coleus Plant Physiol. 121 987 993 Qian, Y.L. Fry, J.D. Upham, W.S. 1997 Rooting and drought avoidance of warm

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-tolerant woody landscape plants J. Environ. Hort. 10 94 99 Pattanagul, W. Madore, M.A. 1999 Water deficit effects on raffinose family oligosaccharide metabolism in coleus Plant Physiol. 121 987 993

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level and expression of genes involved in the ascorbate biosynthesis and recycling pathway in harvested broccoli florets J. Expt. Bot. 56 65 72 Nishizawa, A. Yabuta, Y. Shigeoka, S. 2008 Galactinol and raffinose constitute a novel function to protect

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), and Italy (52,000 tons) ( FAO, 2014 ). Chestnuts are rich in starch and sugars, primarily monosaccharides and disaccharides such as sucrose, glucose, fructose, and raffinose ( Bernardez et al., 2004 ; De la Montana Miguelez et al., 2004 ). In addition

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, raffinose, and some others) are increased under SA application for promoting cold acclimation of glossy privet in the future. Many factors are involved in the cold acclimation process of plants. SA can regulate various aspects of plant responses under both

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acids (4.581–10.180 mg·g −1 dry matter), and organic acids (3.754–6.752 mg·g −1 dry matter) ( Song et al., 2013 ). Zhang et al. (2015) reported that the cumulative contribution to eating quality of sucrose, fructose, glucose, raffinose, stachyose

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