, any leaf, flower, or fruit characteristic is not visible ( Liu et al., 2007 ; Serrano et al., 2002 ). Using DNA markers is an effective and robust method to identify plant material because of its stability in different environmental conditions or in
collections of M. rotundifolia cultivars and VR hybrids. At present, accessions within these collections are documented by comparing breeding records and morphological traits. A molecular marker-based database to aid in establishing a reference collection or
-liang Zhou for instructions of molecular marker experiments, Qi Wang in preparation of Fig.1, Xiao-qing Zhao for meaningful discussion on the taxonomic key, and thoughtful reviews of two anonymous referees.
recent years, V. davidii and their hybrids have been used for making wines and juice in China ( Bao et al., 2010 ; Qin et al., 2008 ; Zhou et al., 2008 ). Molecular genetic markers constitute one of the most powerful tools for analyzing genomes and
polygamodioecy ( Dirr, 1998 ; Nicholson, 1990 ; Ueda, 1996 ) combined with occasional selfing that has implications for crossing and inheritance studies. The development of molecular markers, specifically SSRs, will aid in assessing relationships, diversity
408.040 may provide an additional source of EFB resistance. Current EFB evaluation methods are slow and time-consuming, because cankers cannot be scored until 16–20 months after inoculation. Identification of molecular markers closely linked to EFB
followed by the processes of validation. Somatic hybrids have been identified first by the complementary and/or intermediate morphology of regenerants followed by confirmation of ploidy level, and finally molecular markers are used to confirm the presence
, and Fisheries (SFWF), for access to the software program used in molecular marker analysis. We also thank Thomas Kubisiak, former graduate assistant in LSU SFWF, for technical suggestions. The cost of publishing this paper was defrayed in part by the
collected in Taiwan and appear to be the only H. paniculata germplasm in the United States that was not either introduced from Japan or bred from Japanese germplasm. Microsatellite, or simple-sequence repeat (SSR), markers provide a useful method for
characterized by pomological and chemical traits ( Grati Kamoun et al., 2000 ; Grati Kamoun and Khlif, 2001 ; Mehri et al., 1997 ) with isozyme markers ( Grati Kamoun et al., 1992 , 1999 , 2002 ) and more recently with amplified fragment length polymorphism