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Matthew D. Jeffries, Travis W. Gannon, W. Casey Reynolds, Fred H. Yelverton and Charles A. Silcox

to be soil-incorporated by mechanical/physical or water methods. Following incorporation, dazomet reacts with water to produce methyl isothiocyanate, a gas that controls various fungi, insects, nematodes, and weeds ( Brosnan and Breeden, 2009 ; Unruh

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Dean A. Kopsell and Carl E. Sams

specific chronic ailments including cancer, cardiovascular disease, and age-related macular degeneration ( Giovannucci, 1999 ; Mayne, 1996 ). The most studied bioactive components in the Brassicas are the glucosinolates and their hydrolyzed isothiocyanates

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Harbans L. Bhardwaj and Anwar A. Hamama

, broccoli, and cauliflower ( Brassica sp.) are rich in essential nutrients. In addition, they contain substantial quantities of the glucoside-aglycone sulforaphane or 4-methylsulfinylbutyl isothiocyanate, which are very potent inducers of detoxication

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Mark G. Lefsrud, Dean A. Kopsell and Carl E. Sams

wavelength treatment ( Table 1 ). The enzyme myrosinase catalyzes the hydrolysis of sinigrin to produce allyl isothiocyanate, which reduces the incident of certain cancers ( Stoewsand, 1995 ). Wavelength did not affect the accumulation of any of the other GS

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Xiyan Yu, Xiufeng Wang, Jide Fan, Hongmei Tian and Chengchao Zheng

. Extraction and purification of RNA. Total RNA was isolated using the guanidine isothiocyanate–phenol–chloroform method as described by Sambrook et al. (1989) with modification. Frozen tissue samples (5 to 10 g) were ground to a powder in liquid N 2 using

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Husrev Mennan and Mathieu Ngouajio

glucosinolates, which after enzymatic hydrolysis release isothiocyanates–well known allelopathic substances that may suppress weeds. Weed control without the use of herbicides could be ineffective, expensive, and time-consuming in orchard management and therefore

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Gary S. Bañuelos and Bradley D. Hanson

the potential bioherbicidal effects of Brassica seed meals. Some Brassica seed meals contain nitrogen- and sulfur-containing compounds called glucosinolates (GLS), which can be hydrolyzed into a number of bioactive compounds, including isothiocyanates

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Rachel E. Rudolph, Carl Sams, Robert Steiner, Stephen H. Thomas, Stephanie Walker and Mark E. Uchanski

soil as living plant material ( Kirkegaard et al., 1999 ). The term biofumigation refers to the suppression of soilborne pests and pathogens using naturally occurring biocidal compounds, particularly isothiocyanates (ITCs), which are released from

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André Snyder, Matthew J. Morra, Jodi Johnson-Maynard and Donald C. Thill

isothiocyanate (ITC) on enzymatic hydrolysis ( Larsen, 1981 ), a compound with broad-spectrum toxicity to microorganisms, insects, and plants ( Brown and Morra, 1997 ; Norsworthy and Meehan, 2005 ; Vaughn et al., 2006 ). The dominant GLS in S. alba BSM

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Xinhua Yin, Lynn E. Long, Xiao-Lan Huang, Ngowari Jaja, Jinhe Bai, Clark F. Seavert and Jac le Roux

fruit harvest in Fall 2008. Active and total bacteria numbers were directly estimated using the fluorescein diacetate method ( Ingham and Klein, 1984 ; Lodge and Ingham, 1991 ) and fluorescein isothiocyanate method ( Babiuk and Paul, 1970