produced in controlled environments ( Jaworski et al., 1988 ; Lang and Tibbitts, 1983 ; Petitte and Ormrod, 1986 ; Wetzstein and Frett, 1984 ). For the most part, no pathogen has been found to be involved in intumescence development, which leads most to
Joshua K. Craver, Chad T. Miller, Kimberly A. Williams and Nora M. Bello
Weixing Cao and Theodore W. Tibbitts
This study determined the responses of potato (Solanum tuberosum L., cv. Norland) plants to various patterns of air temperature changes over different growth periods (phasic temperature changes). In each of two experiments under controlled environments, eight treatments of temperature changes were carried out in two growth rooms maintained at 17 and 22C and a constant vapor pressure deficit of 0.60 kPa and 14-hour photoperiod. Plants were grown for 63 days after transplanting of tissue culture plantlets in 20-liter pots containing peat-vermiculite mix. Temperature changes were imposed on days 21 and 42, which were essentially at the beginning of tuber initiation and tuber enlargement, respectively, for this cultivar. Plants were moved between two temperature rooms to obtain eight temperature change patterns: 17-17-17, 17-17-22, 17-22-17, 22-17-17, 17-22-22, 22-17-22, 22-22-17, and 22-22-22C over three 21-day growth periods. At harvest on day 63, total plant dry weight was higher for the treatments beginning with 22C than for those beginning with 17C, with highest biomass obtained at 22-22-17 and 22-17-17C. Shoot dry weight increased with temperature increases from 17-17-17 to 22-22-22C during the three growth periods. Tuber dry weight was highest with 22-17-17C, and lowest with 17-17-22 and 17-22-22C. With 22-17-17C, both dry weights of stolons and roots were lowest. Total tuber number and number of small tubers (<2.5 cm) were highest with 17-17-17 and 17-17-22C, and lowest with 17-22-22 and 22-22-22C, whereas number of medium tubers (2.5-5.0 cm) was highest with 22-17-22C, and number of large tubers (>5.0 cm) was highest with 22-17-17C. This study indicates that tuber development of potatoes is optimized with a phasic pattern of high temperature during early growth and low temperature during later growth.
Jasmine J. Mah, David Llewellyn and Youbin Zheng
In greenhouse ornamental crop production, bedding plants grown below high densities of hanging baskets (HBs) tend to be of lower quality. Hanging basket crops can decrease the red to far red ratio (R:FR) of the growing environment below; however, the extent to which decreased R:FR affects plant morphology and flowering of the lower-level crops is unknown. The present study examined effects of R:FR on morphology and flowering of marigold ‘Antigua Orange’ (Tagetes erecta), petunia ‘Duvet Red’ (Petunia ×hybrida), calibrachoa ‘Kabloom Deep Blue’ (Calibrachoa ×hybrida), and geranium ‘Pinto Premium Salmon’ (Pelargonium ×hortorum). Five R:FR light treatments were provided ranging from R:FR 1.1 (representing unfiltered sunlight) to R:FR 0.7 (representing shaded conditions under HBs) using light-emitting diodes (LEDs) in growth chambers, each with identical photosynthetically active radiation (PAR) (400–700 nm) and FR added to achieve the target R:FR ratio. Two experiments using the same R:FR treatments were conducted with day/night temperature regimes of 20 °C/18 °C and 25 °C/21 °C, respectively. In the second experiment, a fluorescent light treatment was included. The results of the second experiment were more dramatic than the first, where reducing R:FR from 1.1 to 0.7 increased height by 11%, 22%, and 32% in marigold, petunia, and calibrachoa, respectively, and increased petiole length in geranium by 10%. Compared with R:FR 1.1, the R:FR 0.7 shortened the time to the appearance of first flower bud by 2 days in marigold, whereas flowering was minimally affected in other species. Compared with pooled data from the LED treatments, fluorescent light increased relative chlorophyll content for all species, reduced height in marigold, petunia, calibrachoa, and geranium by 26%, 67%, 60%, and 48%, and reduced stem dry weight by 28%, 39%, 21%, and 31%, respectively. The differences in morphology observed under fluorescent light compared with LED R:FR treatments indicate that light quality manipulation is a potential alternative to chemical growth regulators in controlled environments such as greenhouses and growth chambers.
Yoshiaki Kitaya, Genhua Niu, Maki Ohashi and Toyoki Kozai
142 ORAL SESSION 41 (Abstr. 662–667) Controlled Environments–Vegetables
N.C. Yorio, C.L. Mackowiak, R.M. Wheeler and G.W. Stutte
142 ORAL SESSION 41 (Abstr. 662–667) Controlled Environments–Vegetables
Mengmeng Gu, Curt R. Rom, James A. Robbins and Derrick M. Oosterhuis
Water was withheld from 2-year-old seedlings or rooted cuttings of four birch genotypes (Betula alleghaniensis Britton, B. davurica Pall., B. nigra L. ‘Cully’, and B. papyrifera Marsh.) until the combined weight of the container and plant decreased below 40% of its original value to induce plant predawn water potential between −1.5 MPa and −2.1 MPa, after which plants were supplied with a requisite amount of water to reach 40% of its original value for 5 weeks under controlled conditions to investigate changes in gas exchange, osmotic solutes, leaf abscission, and growth compared with well-watered (WW) plants. Observations indicated that three of the four genotypes (except B. papyrifera) expressed three stages of photosynthetic response during water deficit: 1) a stress stage, 2) an acclimation stage, and 3) an adapted (or tolerance) stage. The stages were characterized by decreasing, increasing, and stabilized Pnws/ww (net photosynthesis presented as a ratio of water-deficit stressed (WS) plants to WW plants), respectively. A strong relationship between Pn and g S observed in the WS plants of the four genotypes, suggested inhibition of Pn by stomatal closure. After exposure to water deficit for 5 weeks, Pnws/ww recovered to 70% of the initial value for B. alleghaniensis and B. nigra ‘Cully’ and 98% for B. davurica and B. papyrifera. WS plants had higher foliar concentrations of chlorophyll a and b (nmol/g) and potassium (%) than the WW plants. Increased levels of polyols (mg/g) were detected only in the WS plants of B. allegahaniensis. Increased levels of carbohydrates or organic acid under water deficit were not detected. A significant increase in leaf abscission in the WS plants of B. papyrifera compared with the other genotypes could be a morphological adaptation to water deficit conditions and facilitate recovery of Pnws/ww during the acclimation stage.
Robert W. Langhans and Mauricio Salamanca
With the primary objective of assuring food safety at the production level, a HACCP (Hazard Analysis and Critical Control Point) plan was developed and implemented in an 8000-ft2 greenhouse producing 1000 heads of lettuce per day in Ithaca, N.Y. The plan was developed following the HACCP principles and application guidelines published by the National Advisory Committee on Microbiological Criteria for Foods (1997). The CEA glass greenhouse uses both artificial high-pressure sodium lamps and a shade curtain for light control. Temperature is controlled via evaporative cooling and water heating. Lettuce plants are grown in a hydroponic pond system and are harvested on day 35 from day of seeding. Known and reasonable risks from chemical, physical, and microbiological hazards were defined during the hazard analysis phase. Critical control points were identified in the maintenance of the pond water, the operation of evaporative coolers, shade curtains, and during harvesting and storage. Appropriate prerequisite programs were implemented before the HACCP plan as a baseline for achieving minimum working conditions. Proper critical limits for some potential hazards were established and monitoring programs set up to control them. Postharvest handling was setup in an adjacent head house that was adapted as a food manufacturing facility according to New York State Dept. of Agriculture and Markets standards. Potential applications will be discussed.
C. Michael Bourget
In the area of controlled environment agriculture (CEA), one of the most commonly discussed topics is that of lighting. The lighting system is generally the most expensive component of a CEA facility in terms of upfront costs as well as ongoing
Chase Jones-Baumgardt, David Llewellyn, Qinglu Ying and Youbin Zheng
light signaling. Historically, the most commonly used artificial light sources for controlled environment crop production have been fluorescent tubes in SS environments ( Kozai, 2013 ) and high-intensity discharge lamps such as high-pressure sodium (HPS
Aparna Gazula, Matthew D. Kleinhenz, John G. Streeter and A. Raymond Miller
Pigment concentrations in leaf tissue affect the visual and nutritional value-based indices of lettuce crop quality. To better discern the independent and interactive effects of temperature and cultivar on anthocyanin and chlorophyll b concentrations, three closely related Lolla Rosso lettuce cultivars (`Lotto', `Valeria', and `Impuls'), varying primarily in the number of genes controlling anthocyanin concentrations, were subjected to different air temperatures in controlled environments. Fifteen-day-old seedlings previously grown at 20 °C day/night (D/N) were transplanted into growth chambers maintained at 20 °C (D/N), 30/20 °C D/N and 30 °C D/N air temperatures. Twenty days later, leaf tissue was sampled for measures of pigment concentrations, calculated based on spectrophotometric absorbance readings taken at 530 nm (anthocyanin) and 660 nm (chlorophyll b) respectively. Although significant, the temperature × cultivar interaction resulted from differences in the magnitude (not direction) of the change in pigment concentrations among cultivars with changes in temperature. Regardless of cultivar, anthocyanin and chlorophyll b concentrations were highest, moderate and lowest after growth at 20 °C D/N, 30/20 °C D/N and 30 °C D/N respectively. Likewise, irrespective of temperature, anthocyanin and chlorophyll b concentrations followed the pattern `Impuls' (three genes) > `Valeria' (two genes) > `Lotto' (one gene). These data provide additional strong evidence that lettuce leaf pigment concentrations and growing temperatures are negatively related. The data also suggest that low temperatures during the dark phase may mitigate high temperature-driven reductions in lettuce leaf pigment levels.