pH, 2) to assess the effect of medium pH range on apple subculture proliferation, rooting, and adventitious bud regeneration from leaves, and 3) to determine the necessity of pH adjustment while preparing medium for apple tissue culture. Materials and
measured using a Metrohm 800 Dosino 862 Compact Titrosampler (Metrohm AG, Herisau, Switzerland) and an electrode standardized to pH 2.00, 4.00, 7.00, and 10.00 buffers. Titratable acidity was determined from 6 g of juice diluted with 50 mL deionized
were eluted using a mobile phase consisting 1% (v/v) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. Isocratic elution was performed with a flow rate of 0.75 mL·min −1 . Peaks were detected at 320 nm using a Waters 2487 dual
. Grimsley, N. 1998 Genetic mapping of Ph-2 , a single locus controlling partial resistant to Phytophthora infestans in tomato Mol. Plant Microbe Interact. 11 259 269 10.1094/MPMI.1998.11.4.259 Morrison, L. Skaggs
growth stage, relative leaf elongation rate (RLER) was calculated as followed: where ln PH 2 and ln PH 2 are the natural logarithms of the PH at time one t 1 and time two t 2 , respectively. Plants were harvested from 4.5 m plots when an average of
acidity, combined acidity, and the pH of brine samples were periodically measured in a titroprocessor (Metrohm 670; Herisau, Switzerland). Analyses of free and combined acidity were made by titrating up to pH 8.3 with 0.2 N OHNa, and down to pH 2.6 with 2
present experiment, infusion or postpeeling treatment with 1% citric acid solution (pH = 2.30) reduced surface pH from 5.0 to 5.5 to ≈3.0 to 3.5, which would explain growth suppression of fast-growing spoilage bacteria ( Pao and Petracek, 1997 ). The
samples were collected 39, 74, 94, and 138 DAPF in 2014; and 30, 63, 93, and 122 DAPF in 2015. Soil P was extracted by Mehlich-3 [solution pH, 2.5; soil:solution ratio, 1:10 ( Mehlich, 1984 )], AB-DTPA [solution pH, 7.6; soil:solution ratio, 1
of ethanol 70% and 30 mL of HCl 0.1%, pH 2.0) for 2 min in a blender, then placed in a beaker covered with parafilm and aluminum foil, and extracted in darkness for 12 h at 4 °C. Afterward, a filtration was made, transferring the content to a 250-mL
chelate-associated Fe solubility is 1) a function of substrate pH; 2) chelating agent stability constants with other cations like Ca, Cu, Mn, and Zn; and 3) relative abundance of these cations ( Boxma, 1981 ; de Kreij, 1998 ; Lindsay and Norvell, 1969