Seed weight in lettuce was more important than seed width or thickness in predicting vigor. An air column separator was an effective way to separate seeds varying in weight. A biological assay called the slant test was developed to test vigor. The average root length after 3 days was used as a measure of vigor.
Twenty-eight seedlots of Capsicum annuum from several commercial seed companies were tested for tolerance to low temperatures. Each cultivar was tested three times at 25, 20, and 15°C in laboratory incubators. It was observed that while high germination percentages (r85%) and fast germination rates were found in several seedlots, the relationship was not strongly linked to type of chile, age of seed, or treatment temperature. Of the 28 seedlots, 14 came from companies which are in the top 10% in volume and sales. The other 14 seedlots were from smaller companies. Seventy-one percent of the seedlots with germination at r85% came from the top ten companies. Ninety-two percent of the seedlots with s85% came from small companies. To further test this finding, we randomly choose six seedlots from a small company and 6 seedlots from a small company. There was a clear delineation in germabiltiy between the small company and the large company. Our results indicate a trend that could have a negative impact on some chile seed markets. Electrical conductivity (EC) is commonly used to detect membrane leakage in seeds. Chile seed from 12 seedlots (6 from the large company and 6 from the small company) were soaked 18 hours at 25 and 5°C. There was a negative correlation (r2 = 0.76) between the 15°C germination at 40 days and the EC. It is important to determine why membrane leakage varies in seedlots from different companies and whether the leakage is due to phenotypic or cultural factors, or due to management practices within the company.
Our objective was to identify QTL for seed weight (SW), length, and height segregating in a recombinant inbred line (RIL) population from the cross `PC-50' (Larger SW) × XAN-159 (Smaller SW). The parents and RILs were grown in two separate greenhouse experiments in Nebraska, and in field plots in the Dominican Republic and Wisconsin. Data analysis was done for individual environments separately and on the mean over all environments. A simple linear regression analysis of all data indicated that most QTL appeared to be detected in the mean environment. Composite interval mapping (CIM) analysis was then applied to the means over environments. Eight QTL for SW were detected on common bean linkage groups (LGs) 3, 4, 5, 6, 7, and 8. All eight markers associated with these QTL were significant in a multiple regression analysis (MRA), where the full model explained 63% of the variation among SW means. Six QTL for seed length were detected on LGs 2, 3, 4, 8, and 11 using CIM. The markers associated with the three seed length QTL on LGs 2, 8, and 11 were significant in a MRA with the full model explaining 48% of the variation among seed length means. Three QTL for seed height on LGs 4, 6, and 11 explained 36% of the phenotypic variation for trait means. Four of the six QTL for seed length and two of three QTL for seed height also appeared to correspond to QTL for SW.
Replicated field plots of small, medium, and large pimiento seeds, cv. Truhard Perfection, were planted in mid-February of 1972 and 1973. Compared to seedlings produced by small seeds, those from medium and large seeds emerged 2 days earlier to a significantly better stand, and attained in 71 days the growth, the hardened condition, and the root system required of usable transplants by canners. Transplants from small seeds failed to reach these requirements within the 75-day duration of this study.
Determination of plant growth regulator accumulation in fruits and vegetables for human consumption is an important safety issue even when it is applied to seeds. Paclobutrazol accumulated preferentially in the seedcoats when soaking cucumber (Cucumis sativus L., cv. Poinsett 76SR) seeds in 1000 or 4000 mg·L–1 paclobutrazol. Cucumber plants grown from seeds soaked in 1000 mg·L–1 paclobutrazol had lower average fruit weights than the control plants. Individual fruit length in cucumber was reduced by 40% when seeds were soaked in 1000 mg·L–1 paclobutrazol solutions for 180 minutes. Soaking tomato (Solanum lycopersicum L., cv. Sun 6108) seeds in 0 to 1000 mg·L–1 paclobutrazol did not reduce average fruit weight or diameter per treatment. Paclobutrazol residue was not detected in cucumber and tomato fruits harvested from plants grown from seeds soaked in 1000 mg·L–1 paclobutrazol for 180 minutes. Soaking seeds in paclobutrazol solutions represents a promising method of applying plant growth regulators to tomato and cucumber without accumulation of paclobutrazol residue in fruits.
and soups ( Gusmini et al., 2004 ; Zhang, 1996 ). The seed of the egusi watermelon is high in oil and is characteristically large in size with a fleshy pericarp but the origin of this phenotype remains uncertain ( Gusmini et al., 2004 ). The egusi
measure, hacksaw or pipe cutter, scissors, utility knife, flat-head screw driver, socket driver for adjusting the hose clamps, pliers with a wire cutter, drill with a range of small bits (i.e., 1/16 inch or smaller depending on the size of the seed to be