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)] in tap water (pH 2.8, EC 0.49 dS·m −1 ); 3) 10 g·L −1 a propriety mixture of sugar, acidifier and a biocide [FLO (Floralife ® Crystal Clear packets; Floralife, Walterboro, SC)] in tap water (pH 3.1, EC 0.50 dS·m −1 ); 4) deionized water (pH 3.8, EC

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adjusted to a pH of 6 to 7 and was partitioned against hexane three times. The pooled aqueous residue was then adjusted to pH 2.5 with HCl and partitioned against ethyl acetate. The pooled ethyl acetate phase was partitioned against a potassium phosphate

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-pure water. Sample and standard preparation. To extract organic acids, we precision-weighed 5.0 g of V. uliginosum fruit from each sample and added 5 mL of mobile phase (0.01 mol·L −1 K 2 HPO 4 , pH 2.5) before grinding the sample into a homogenate. We

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interlinked columns Supelco LC-18 (250 × 4 mm); 30 °C; mobile phase: 1% phosphoric buffer (pH = 2.5), flow rate: 0.8 mL·min −1 . Anthocyanins were determined by pH differential method ( Wrolstadt, 1976 ) with Cary 300E spectrophotometer (Varian, Australia

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Waters 600E pump (Waters Corp., Milford, MA). Phenolic acids were eluted using a mobile phase consisting of 1% (by volume) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. The column temperature was set to 30 °C and an

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.L. Methods of soil analysis. Part 2 2nd Ed Agron. Monogr. 9. ASA and SSSA Madison, WI Landschoot, P. 2007 Managing soil pH in turf Grounds Maintenance, Penton Media, Inc 21 Nov. 2007 < http://grounds-mag.com/mag/grounds_maintenance_managing_soil_ph

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chromatography (HPLC) separation of tartaric, malic, and oxalic acids was achieved using a Prevail Organic Acid column 250 × 4.6 mm, 5-μm (Alltech Associates, Deerfield, IL); the mobile phase was 25 m m KH 2 PO 4 adjusted to pH 2 with phosphoric acid at a flow

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each harvest date. Soluble solids content was calculated using a refractometer. Titratable acidity and pH were measured by Metrohm 800 Dosino 862 compact titrosampler and electrode standardized to pH 2.00, 4.00, 7.00, and 10.00 buffers (Metrohm AG

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. Fig. 1. Relationship between substrate pH and chlorophyll meter readings (SPAD-502; Minolta, Ramsey, NJ) or shoot dry weight (SDW) of ‘Pacifica Blush’ annual vinca grown in switchgrass substrates; SPAD = −2.29 × pH 2 + 29.10 × pH − 38.09, R 2 = 0

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pathway ( Forkmann, 1991 ; Holton and Cornish, 1995 ; Mol et al., 1998 ; Wiering and deVlaming, 1984 ). Additionally, genetic differences in flower color resulting from modifications in the pH were explained by genes Ph1, Ph2 , and Ph6 ( Griesbach

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