-pure water. Sample and standard preparation. To extract organic acids, we precision-weighed 5.0 g of V. uliginosum fruit from each sample and added 5 mL of mobile phase (0.01 mol·L −1 K 2 HPO 4 , pH 2.5) before grinding the sample into a homogenate. We
)] in tap water (pH 2.8, EC 0.49 dS·m −1 ); 3) 10 g·L −1 a propriety mixture of sugar, acidifier and a biocide [FLO (Floralife ® Crystal Clear packets; Floralife, Walterboro, SC)] in tap water (pH 3.1, EC 0.50 dS·m −1 ); 4) deionized water (pH 3.8, EC
models, Eqs. [3] through [7] , were obtained by fixing three factors as the zero levels, whereas the other three factors were set as an explanatory variable (as shown in Figs. 2 and 3 ): y PH = 2 . 696 + 0.295 w + 0 . 216 w 2 [3] y CD = 8 . 414 + 0
were eluted using a mobile phase consisting 1% (v/v) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. Isocratic elution was performed with a flow rate of 0.75 mL·min −1 . Peaks were detected at 320 nm using a Waters 2487 dual
chromatography (HPLC) separation of tartaric, malic, and oxalic acids was achieved using a Prevail Organic Acid column 250 × 4.6 mm, 5-μm (Alltech Associates, Deerfield, IL); the mobile phase was 25 m m KH 2 PO 4 adjusted to pH 2 with phosphoric acid at a flow
.L. Methods of soil analysis. Part 2 2nd Ed Agron. Monogr. 9. ASA and SSSA Madison, WI Landschoot, P. 2007 Managing soil pH in turf Grounds Maintenance, Penton Media, Inc 21 Nov. 2007 < http://grounds-mag.com/mag/grounds_maintenance_managing_soil_ph
Waters 600E pump (Waters Corp., Milford, MA). Phenolic acids were eluted using a mobile phase consisting of 1% (by volume) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. The column temperature was set to 30 °C and an
interlinked columns Supelco LC-18 (250 × 4 mm); 30 °C; mobile phase: 1% phosphoric buffer (pH = 2.5), flow rate: 0.8 mL·min −1 . Anthocyanins were determined by pH differential method ( Wrolstadt, 1976 ) with Cary 300E spectrophotometer (Varian, Australia
each harvest date. Soluble solids content was calculated using a refractometer. Titratable acidity and pH were measured by Metrohm 800 Dosino 862 compact titrosampler and electrode standardized to pH 2.00, 4.00, 7.00, and 10.00 buffers (Metrohm AG
pH, 2) to assess the effect of medium pH range on apple subculture proliferation, rooting, and adventitious bud regeneration from leaves, and 3) to determine the necessity of pH adjustment while preparing medium for apple tissue culture. Materials and