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Thomas C. Koch and Irwin L. Goldman

Carotenoids and tocopherols are health-functional phytochemicals that occur in a wide range of fruit and vegetable crops. These two classes of compounds are synthesized from a common precursor, geranyl-geranyl pyrophosphate, and are typically analyzed separately via high-performance liquid chromatography (HPLC) techniques. Because carotenoids and tocopherols are present in many edible horticultural crops, it would be advantageous to measure them simultaneously in plant tissues. Herein we report a one-pass reverse-phase HPLC method for extraction and analysis of carotenoids and tocopherols in carrot that can be extended to other high-moisture plant organs. Elution times ranged from 5 minutes for α-tocopherol to 24 minutes for β-carotene. This method improves the efficiency of analyzing these compounds by up to 50%, and should increase the efficiency of assessing carotenoid and tocopherol profiles in horticultural crops.

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Fekadu Gurmu, Shimelis Hussein and Mark Laing

another major focus area, among which improving β-carotene content (provitamin A) is the top priority. Breeding for high β-carotene content is crucial because vitamin A deficiency (VAD) is a serious health problem that results in blindness, weak resistance

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Muttalip Gündoğdu, Tuncay Kan and Mustafa Kenan Gecer

phenolic content is found to be correlated with reduced incidences of heart diseases in epidemiological studies ( Criqui and Ringel, 1994 ; Renaud and de Lorgeril, 1992 ). The anticarcinogenic and antioxidant properties of vitamins A, C, E, β-carotene, and

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Christine M. Bradish, Gad G. Yousef, Guoying Ma, Penelope Perkins-Veazie and Gina E. Fernandez

flow rate of 1 mL·min −1 using a step gradient of 0 min, 90% A, 10% B; 24 min, 54% A, 35% B, 11% C; 35 min, 30% A, 35% B, 35% C; 43–58 min, 90% A, 10% B. Calibration curves were determined using external standards of α-carotene, β-carotene, 9-cis-β-carotene

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Laura Rodriguez-Uribe, Luz Hernandez, James P. Kilcrease, Stephanie Walker and Mary A. O’Connell

, capsanthin is the most abundant carotenoid and this carotenoid generates the red color in the fruit ( Daood et al., 2014 ; Deli et al., 2001 ). β-carotene and β-cryptoxanthin are orange-colored carotenoids that are essential provitamin A nutrients. They are

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Chae Shin Lim, Seong Mo Kang, Jeoung Lai Cho and Kenneth C. Gross

. Lycopene and β-carotene contents and antioxidizing enzyme activities were measured at the end of the storage. After storage, fruit was cut into small pieces, frozen in liquid N 2 , and stored at –70 °C for enzyme assays. Surface pitting. Visible CI

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Shiva Ram Bhandari, Bo-Deul Jung, Hum-Young Baek and Young-Sang Lee

flavonoids and water content were measured using fresh samples. Vitamin E, squalene, phytosterols, fatty acids, β-carotene, and antioxidant activity were analyzed after the samples were dried in an oven at 55 °C for 72 h and ground into a fine powder. The

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James T. Brosnan, Dean A. Kopsell, Matthew T. Elmore, Gregory K. Breeden and Gregory R. Armel

The six primary carotenoids found in most plant species include zeaxanthin, antheraxanthin, violaxanthin, lutein, β-carotene, and neoxanthin ( Sandmann, 2001 ). Carotenoids are pigments integrated into light-harvesting complexes of chloroplasts that

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Cristián Vela-Hinojosa, Héctor B. Escalona-Buendía, José A. Mendoza-Espinoza, Juan M. Villa-Hernández, Ricardo Lobato-Ortíz, Juan E. Rodríguez-Pérez and Laura J. Pérez-Flores

lycopene (≈90%), β-carotene (5% to 10%) and lutein (1% to 5%), with only trace concentrations of other compounds ( Stahl and Sies, 2003 ). In recent years, the biosynthetic pathways of carotenoid, tocopherol, and chlorophyll have been studied because of

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Zoltán Pék, Lajos Helyes and Andrea Lugasi

predominant carotenoid lycopene, which causes red coloration of fruits, is characterized well by a* parameter. β-carotene is an orange colorant of fruits, in which the parameter is measurable by b* in the CIELab color system ( Sacks and Francis, 2001 ). The