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Xinyi Zhang, Li Liao, Zhiyong Wang, Changjun Bai and Jianxiu Liu

), amplified fragment length polymorphisms (AFLPs), and simple sequence repeats (SSRs) ( Kalia et al., 2011 ). Intersimple sequence repeat markers can be amplified simply and directly without knowledge of the flanking sequences, thus enabling easier development

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Yan Liu, Hailin Guo, Yi Wang, Jingang Shi, Dandan Li, Zhiyong Wang and Jianxiu Liu

; Wang et al., 2011 ). Several molecular markers, such as restriction fragment length polymorphism markers, simple sequence repeat markers, amplified fragment length polymorphism markers, and RAPD markers have been used to research the genetic diversity

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Sima Taheri, Thohirah Lee Abdullah, Nur Ashikin Psyquay Abdullah and Zaiton Ahmad

stability and reproducibility, rich polymorphism, reliability, much larger numbers of fragments per primer, and relatively low cost, they have been widely used for DNA fingerprinting, population genetics, and phylogenetic studies ( Zhou et al., 2007

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Jie Fu, Qiaoyan Xiang, Xianbao Zeng, Mei Yang, Ying Wang and Yanling Liu

amplified fragment length polymorphism (AFLP) group for 138 lotus accessions analyzed by AFLP. Amplified fragment length polymorphism analysis. Approximately 2 g of fresh young leaves were harvested from plants and used for the extraction of total genomic

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Ashish K. Pathak, Sudhir P. Singh and Rakesh Tuli

fingerprint and classify lychee cultivars using isozymes ( Aradhya et al., 1995 ; Degani et al., 1995 ) and DNA polymorphism using random amplified polymorphic DNA [RAPD ( Anuntalabhochai et al., 2002 ; Chundet et al., 2007 ; Kumar et al., 2010

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Daofeng Liu, Jing Ma, Jianfeng Yang, Tien V. Nguyen, Huamin Liu, Renwei Huang, Shunzhao Sui and Mingyang Li

polymorphism and can easily be amplified by polymerase chain reaction (PCR) using primers designed from flanking sequences of the SSR motifs. SSR markers have been useful for integrating the genetic, physical, and sequence-based physical maps in plant species

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Gen-Fa Zhu and Dong-Mei Li

, needs no cloning and sequencing, and produces a large number of recordable fragments, which enhance its power to detect polymorphism ( Vos et al., 1995 ). The AFLP technique had been successfully used in the estimation of genetic relationships and

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C. Degani, A. Beiles, R. El-Batsri, M. Goren and S. Gazit

Leaf isozyme banding patterns were studied in 30 cultivars and selections of lychee (Litchi Chinensis Sonn.) by means of starch gel electrophoresis. Polymorphism in aconitase, aspartate aminotransferase, isocitrate dehydrogenase, phosphoglucomutase, shikimate dehydrogenase, superoxide dismutase and triosephosphate isomerase is demonstrated for the first time and observations are extended for the previously described polymorphism in phosphoglucose isomerase. In this study we found five groups of cultivars with identical electrophoretic genotypes. The 18 different cultivars were clustered by the UPGMA method into two large clusters and three pairs of similar cultivars. Three cultivars were relatively separate from the clusters. This study shows that isozyme polymorphism is a prevalent phenomenon in lychee, and that isozymes can provide useful genetic markers for lychee cultivar identification and parental analysis.

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Thomas S. Brettin and Amy F. Iezzoni

Sour cherry (Prunus cerasus) is an allotetraploid with sweet cherry (P. avium) and ground cherry (P. fruticosa) as the proposed progenitor species. Three cpDNA markers from eight sweet, four ground, and 26 sour cherry selections were analyzed to investigate the relatedness of their cp genomes. To date, two RFLP polymorphisms have been identified with both the P2 and P4 fragments of tomato cpDNA, while four length polymorphisms of an intergenic spacer have been identified by PCR amplification. Sweet and ground cherry have different cp polymorphisms, while sour cherry individuals have been identified that have the sweet and ground cherry polymorphisms plus a unique polymorphism. Additional individuals chosen to represent the diversity within each species will be screened to provide a more complete assessment of cp diversity. In addition, progeny from a sour cherry cross where the parents have different cp polymorphisms are being evaluated to determine if the chloroplasts are exclusively maternally inherited.

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Rui Li, Lu Fan, Jingdong Lin, Mingyang Li, Daofeng Liu and Shunzhao Sui

to 50 ng·μL –1 , and DNA was stored at –20 °C for the next experiment. The SCoT30-80 primers ( Luo et al., 2010 ) were ordered from TsingKe (Chengdu, China). All primers were screened with wild-type kalanchoe materials for polymorphisms and