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Lisa J. Rowland, Elizabeth L. Ogden, Fumiomi Takeda, David Michael Glenn, Mark K. Ehlenfeldt and Bryan T. Vinyard

open flowers of five highbush cultivars, Bluecrop, Elliott, Hannah’s Choice, Murphy, and Weymouth, was determined using two different freezing methods. Comparisons of the methods and genotypes were performed based on the freezing tolerances of several

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Erik J. Landry and David J. Wolyn

herbaceous, the “stay green” phenotype characteristic of JG in southern Ontario could predispose this cultivar to deficiencies in cold acclimation, freezing tolerance, and consequently longevity. The phenology of cold acclimation for asparagus ( Krug, 1999

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Bing Liu, Hong Zhou, Sha Cao, Yi-ping Xia and Rajeev Arora

freezing tolerance (FT) via a process called cold acclimation which is induced by exposure to short days and cooler temperatures in autumn ( Arora et al., 1992 ). This induced FT is then lost via a process called deacclimation when warmer temperatures

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Rajeev Arora and Lisa J. Rowland

perennials to increase their freezing tolerance when nonacclimated (NA) tissues are exposed to inductive cues such as short days and/or low temperatures (as in the fall). It is one of the most-studied and best-characterized responses using both model systems

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Lisa J. Rowland, Elizabeth L. Ogden, Mark K. Ehlenfeldt and Rajeev Arora

complex interacting factors. They include the timing of dormancy induction in the summer, the timing and rate of cold acclimation in the fall, the level of freezing tolerance reached while plants are in the cold-acclimated state, the maintenance of

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H.W. Philley, C.E. Watson Jr., J.V. Krans, J.M. Goatley Jr. and F.B. Matta

The objective of this study was to relate the lethal freezing temperatures of St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] genotypes, as measured by differential thermal analysis (DTA), to winter survival observed in the field. DTA-predicted lethal temperatures of 14 St. Augustinegrass genotypes ranged from –7.7 to –4.7C. Regression of field winter survival vs. DTA-predicted lethal temperatures resulted in an r 2 = 0.57 for one field trial that evaluated cultivars with a relatively narrow range of expected freezing tolerance. In a second study evaluating cultivars with a greater range of freezing tolerance, r 2 was 0.92 when winter survival was regressed on DTA-predicted lethal temperatures. DTA was successful in measuring freezing avoidance of St. Augustinegrass cultivars.

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Tim D. Davis, James E. Ells and Ronald H. Walser

Seeds of Lycopersicon esculentum Mill. cv. UC 82L were treated with hypertonic priming solutions containing KNO3 and K3PO4(10 g·liter-1 each), and various concentrations of uniconazole before sowing. Treatment of the seed with priming solution only hastened emergence by ≈ 2 days compared to untreated seed sown directly from the packet, but did not affect total emergence after 12 days. Addition of uniconazole to the priming solution had no significant effect on speed of emergence or total emergence after 12 days compared to the primed control. Seed priming plus uniconazole at 1 or 10 mg·liter-1 reduced seedling height after 2 weeks by ≈ 20% compared to the primed control. Uniconazole had no effect on the mortality of either hardened or nonhardened seedlings exposed to below-freezing temperatures for 3 hr. These data suggest that treatment of tomato seed with hypertonic solutions containing uniconazole would be of little practical value in protecting seedlings from freeze damage. Chemical names used: (E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-yl)penten-3-ol (uniconazole).

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Tomasz Anisko, Cynthia L. Haynes and Orville M. Lindstrom

Freeze tests were performed on stem sections of Fraxinus americana, Lagerstroemia indica Magnolia gradiflora, Rhododendron `Red Ruffle', Zelkova serrata, and leaves of Magnolia grandiflora and Rhododendron `Red Ruffle' in the tinter and summer of 1993. Freeze injury was quantified using electrolyte and phenolic leakage techniques and compared to the lethal temperature range determined by visual method assisted by differential thermal analysis. Richards function was fitted to the electrolyte and phenolic leakage data by the modified Gauss-Newton method. The inflection point of the Richards function coincided with the lethal injury range for non-acclimated leaves, but overestimated the freeze tolerance for acclimated leaves and for both acclimated and non-acclimated stems. A proposed interception point of the lower asymptote and a line tangential to the curve inflection point provided an improved estimate of the lethal injury range in most of the species.

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Edward Bush, Paul Wilson, Dennis Shepard and James McCrimmon

An experiment to determine the nonstructural carbohydrate composition and nodal survival (LT50) of common carpetgrass was conducted between 1993 and 1994 at Baton Rouge, La. Nonstructural carbohydrates in stolons were primarily sucrose [70-130 mg·g-1 dry weight (DW)] and starch (8-33 mg·g-1 DW). Total nonstructural carbohydrate (TNC) composition of stolons ranged between 30 to 165 mg·g-1 DW. Node survival following exposure to 2 °C ranged from 0% in August-sampled grass to 48% in December. The LT50 following acclimation under field conditions was -2 to -4 °C. Environmental factors influenced nonstructural carbohydrate composition, partitioning, and node survival. No relationship between TNC concentration and low-temperature tolerance was found. This research confirms previous reports that low-temperature tolerance of carpetgrass is very poor, and its culture may be limited to geographical areas having moderate winter temperatures.

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Philip Busey

St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] has low freezing tolerance and suffers winter injury in the southeastern United States. Laboratory methods have determined that the lethal cold temperature of St. Augustinegrass stolons and buds is between -4.5 °C and -7.7 °C. The field survival of St. Augustinegrass to winter freezing is poorly known because most field reports have been based on a single location experiencing a single winter minimum air temperature. The objective of the study was to assess the winter survival of St. Augustinegrass cultivars across a range of winter minimum air temperatures occurring in experimental plantings at 24 Florida counties, following a severe Arctic cold front that moved through Florida beginning 21 Dec. 1989. Except for two counties, the limit for St. Augustinegrass winter survival was a minimum air temperature between -6 °C and -9 °C. Based on a nonlinear estimate using a 3-parameter sigmoidal model (r 2 = 0.70, P < 0.0001), 50% survival of St. Augustinegrass would be predicted at -7.9 °C. Time since planting had no relationship with survival. Differences among St. Augustinegrass cultivars were observed at only two counties.