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complement) was analyzed with a Partec PA-II flow cytometer (Partec, Münster, Germany). Holoploid, 2C genome size was calculated as: 2C = genome size of standard × (mean fluorescence value of sample/mean fluorescence value of standard). Evaluating

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using a Partec PA II flow cytometer (Partec, Görlitz, Germany) to determine genome size. Holoploid, somatic, sporophytic, unreduced 2C genome size was calculated as the DNA content of the standard (pg) × (mean fluorescence value of the sample / mean

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-cedar cultivars. Table 1. Source and relative genome size of Cryptomeria japonica taxa based on flow cytometry of 4′,6-diamidino-2-phenylindole-stained nuclei using Pisum sativum ‘Ctirad’ (2C = 8.76 pg) as an internal standard. Flow cytometry. Approximately

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by a mercury arc lamp (PA-I Ploidy Analyzer; Partec). The mean fluorescence of each sample was compared with an internal standard of known genome size ( Pisum sativum L. ‘Ctirad’, 2C = 8.76 ρg; Greihuber et al., 2007 ). Base 1Cx monoploid DNA

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the internal standard to determine holoploid genome size [2 C -value: DNA content of the whole complement of chromosomes characteristic for the organism, irrespective of the ploidy level ( Greilhuber et al., 2005 )]. At least 3000 nuclei were counted

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Products, Eugene, OR) under an unheated polyhouse with regular overhead irrigation. Flow cytometry. Flow cytometry was used to confirm ploidy level of study plants. We calculated holoploid (2C) genome size of individual accessions of Vaccinium

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by Palmer et al. (2009) . The mean fluorescence of each sample was compared with an internal standard of known genome size: Pisum sativum L. ‘Ctirad’, 2C = 8.76 pg ( Greihuber et al., 2007 ). Evaluating male fertility. Fresh pollen was

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interploidy crosses had intermediate genome sizes ranging from 2.35 to 2.43 pg ( Table 1 ), and they had morphology shared with the pollen parent (e.g., pink flowers and leaf morphology). ‘Emerald Beauty’ and ‘Emerald Sprite’ were originally accessioned and

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counts per sample. Holoploid, 2C genome size was calculated as: 2C = genome size of standard × (mean fluorescence value of sample/mean fluorescence value of standard). Cytology was conducted on an individual accession from each subgroup, determined in the

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responses to continuous variables (PROC GLM; SAS Version 9.1; SAS Inst.). Determining tissue ploidy level. Holoploid, 2C DNA content (i.e., DNA content of the entire nonreplicated, chromosome complement), and associated ploidy level were determined

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