A multiplicative model of stomatal conductance was developed and tested in two functionally distinct ecotypes of Acer rubrum L. (red maple). The model overcomes the main limitation of the commonly used Ball-Berry model by accounting for stomatal behavior under soil drying conditions. It combined the Ball-Berry model with an integrated expression of abscisic acid-based control mechanisms (gfac). The factor gfac = exp(-β[ABA]L) incorporated the stomatal response to abscisic acid (ABA) concentration in the bulk leaf tissue [ABA]L into the Ball-Berry model by down-regulating the slope and coupled physiological changes at the leaf level with those of the root. The stomatal conductance (gs) down regulation is pertinent in situations where soil drying may modify the delivery of chemical signals to leaf stomates. Model testing results indicated that the multiplicative model was capable of predicting stomatal conductance under wide ranges of soil and atmospheric conditions in a woody perennial. Concordance correlation coefficients (rc) were high (between 0.59 and 0.94) for the tested ecotypes under three different environmental conditions (aerial, distal, and minimal stress). The study supported the use of the gfac factor as a gas exchange function that controlled water stress effects on gs and aided in the prediction of gs responses.
John R. Yeo, Jerry E. Weiland, Dan M. Sullivan and David R. Bryla
Phytophthora cinnamomi Rands is a ubiquitous soilborne pathogen associated with root rot in many woody perennial plant species, including highbush blueberry (Vaccinium corymbosum). To identify genotypes with resistance to the pathogen, cultivars and advanced selections of highbush blueberry were grown in a greenhouse and either inoculated or not with propagules of P. cinnamomi. Two experiments were conducted, including one with 10 commercially established cultivars and another with seven newly released cultivars, three commercially established cultivars, and three advanced selections of highbush blueberry. Pathogen resistance was based on the shoot and root dry biomass of the inoculated plants relative to the noninoculated plants within each genotype, as well as on the percentage of root infection among the genotypes. Resistant genotypes included four commercially established cultivars, Aurora, Legacy, Liberty, and Reka, and two new cultivars, Overtime and Clockwork. When these genotypes were inoculated, average relative shoot biomass was ≥60% of that of the noninoculated plants, whereas relative root biomass was ≥40%. ‘Star’, as well as two advanced selections (an early- and a late-season type) may also have some degree of resistance, but further investigation is needed. Relative shoot biomass of the susceptible genotypes, on the other hand, ranged from 19% to 53% and relative root biomass ranged from 11% to 26%. The susceptible genotypes included ‘Bluetta’, ‘Bluecrop’, ‘Bluegold’, ‘Blue Ribbon’, ‘Cargo’, ‘Draper’, ‘Duke’, ‘Elliott’, ‘Last Call’, ‘Top Shelf’, and ‘Ventura’. These cultivars are not recommended at sites with conditions conducive to root rot, such as those with clay soils and/or poor drainage.
Bruce Schaffer, Frederick S. Davies and Jonathan H. Crane
The effects of flooding calcareous soil on physiology and growth have been studied for several subtropical and tropical fruit crops including avocado (Persea americana Mill.), mango (Mangifera indica L.), carambola (Averrhoa carambola L.), and several Annona species. In calcareous soils that have a high pH, short-term flooding can actually be beneficial to subtropical and tropical fruit crops by increasing the solubility of particle-bound nutrient elements such as Fe, Mn and Mg due to flooding-induced decreases in soil pH. Additionally, flooding reduces the redox potential in the soil, resulting in Fe being reduced from Fe3+ to Fe2+, which is the cation metabolized by plants. As with other woody perennial crops, one of the early physiological responses of subtropical and tropical fruit trees to flooding is a decrease in stomatal conductance and net CO2 assimilation. If the flooding period is prolonged, lack of O2 (anoxia) in the soil results in a reduction of root and shoot growth, wilting, decreased nutrient uptake and eventual death. The flooding duration required to cause tree mortality varies among species, among cultivars within species, and with environmental conditions, particularly temperature. Several tropical and subtropical fruit crops have anatomical or morphological adaptations to tolerate prolonged flooding, such as development of hypertrophied stem lenticels, adventitious rooting or formation of porous aerenchyma tissue. For grafted trees, flooding-tolerance is conferred by the rootstock and not the scion. Therefore there is a possibility to increase flood tolerance of subtropical and tropical fruit crops by identifying or developing flood-tolerant rootstocks.
Michael V. Mickelbart and Thomas E. Marler
Sapodilla [Manilkara zapota (L.) Royen], reportedly tolerant of saline conditions relative to other tropical fruit species, was studied in sand culture under greenhouse conditions to examine the physiology of sapodilla trees exposed to NaCl and to aid in determining the basis for this apparent tolerance. Treatments, consisting of a complete nutrient solution of 1 dS·m–1 (control) or this solution amended to 12 or 20 dS·m–1 with NaCl, were administered from 16 Nov. 1991 until 29 Jan. 1992. Net CO2 assimilation (A) of plants receiving NaCl gradually decreased relative to that of the control plants. At the end of 8 weeks of salinity, A of plants receiving 12 or 20 dS·m–1 was 72% or 31% of control plants, respectively. Substrate NaCl reduced apparent quantum yield, photosynthetic CO2-use efficiency, leaf osmotic potential, and predawn xylem potential of sapodilla leaves. Dark respiration and the variable: maximal chlorophyll fluorescence ratio were not influenced by NaCl. Exposure to NaCl also increased leaf tissue Na+ and Cl– concentrations and the Na+: K+ ratio. These results indicate that gas exchange of sapodilla is relatively low for woody evergreen species. Moreover, sapodilla may not be as tolerant of salt stress as previously reported. The responses of sapodilla to root zone NaCl were consistent with other woody perennial glycophyte species. Photochemical efficiency of leaves on plants receiving NaCl was not different from that of leaves on control plants for >8 weeks after NaCl reduced gas exchange.
Gary D. Coleman, Brent L. Black and Leslie H. Fuchigami
Temperate woody perennials produce proteins in the stem for seasonal nitrogen (N) storage. In Populus species, this seasonal N storage occurs primarily as a 32-kDa Bark Storage Protein (BSP), which can accumulate to 50% of total bark proteins during the winter. Plants of the Populus tremula × Populus alba (clone 717) were transformed with the BSP cDNA in antisense orientation (fused to a constitutive promoter), and regenerated lines were screened. Several independent antisense-BSP (A-BSP) lines were selected, which, after 4 weeks of SD photoperiod, showed 70% to 90% reduction in total BSP accumulation compared to the wild-type (WT). A series of experiments were conducted to compare LD growth of one A-BSP line to that of the WT. A-BSP plants showed reduced growth at both 5 and 50 mM ammonium nitrate fertilization. However, the higher N rate eventually resulted in toxicity in WT, but not in A-BSP plants. A-BSP plants grown hydroponically (0.5x Hoagland1s) showed altered partitioning with reduced stem length and increased leaf area (Leaf:stem dry-weight ratios were 14.8 and 20.9 for ABSP and WT, respectively). Partitioning to the roots was not different between A-BSP and WT. Proposed functions of BSP in seasonal and LD nitrogen metabolism will be discussed.
Rajeev Arora, Lisa J. Rowland, Ganesh R. Panta, Chon-Chong Lim, Jeffrey S. Lehman and Nicholi Vorsa
Mode of inheritance of cold hardiness (CH) in woody perennials is not wellunderstood. This study was undertaken to determine the mode of inheritance and gene action of CH in blueberry (Vaccinium section Cyanococcus). Two testcross populations (segregating for CH) derived from interspecific hybrids of V. darrowi (drw) × V. caesariense (csr) were used. Plants were cold-acclimated by a 4-week exposure to 4°C. Bud CH (LT50) was defined as the temperature causing 50% injury (visual) when subjected to controlled freeze–thaw. Results show that the drw and csr parents had an LT50 of –13° and –20°C, respectively. The F1 population exhibited mean LT50 of –14.7°C. The csr and drw testcross populations had a mean LT50 of –18° (39 individuals) and –14°C (33 individuals), respectively. Individuals of each population were distributed between parental values with center of distribution skewed toward the testcross parent. Since individuals having LT50s as same as the recurrent parents were present in each population of only 33–39 plants, data suggest that CH is determined by relatively few genes. To determine gene action, the estimates for various genetic parameters (calculated from joint scaling test) were used in generation means analysis to test various models. Results indicate that CH in blueberry can be best explained by simple-additive dominance model, whereas models including epistatic components did not satisfactorily explain the data.
Gail E. Barth
A research program is being conducted to support the development of superior varieties of Ixodia achillaeoides for cut flower production. This species is an everlasting daisy in the Asteraceae, which is produced on a woody perennial bush and is currently both harvested from the wild and cultivated in Southeastern and Western Australia. Ixodia shows a high degree of variation in plant form, flower characteristics, and flowering dates throughout its geographic distribution. In our assessment program, seedlings are screened for a range of morphological and flowering characteristics, and clonal selections are established under cultivation to assess suitability to row culture. The goal is the development of selections with known flowering characteristics and disease tolerance for fresh and dried flower markets and for flowering pot plants. Description is given of assessment criteria for selection of varieties for dried and fresh markets. Seventy selected varieties are currently being assessed in randomized block plantings at two sites in South Australia. Preliminary results and descriptions are presented for superior selections made for dried flower markets. The postharvest performance of selections for fresh markets will be discussed. Research on control of flowering will be presented.
Anne Fennell, M.J. Line and M. Faust
Changes in water status have been associated with various stages of dormancy and freezing tolerance in woody perennials. Recent studies in apple indicate that changes in the state (bound vs. free) of bud water are strongly correlated with the end of dormancy. In this study nuclear magnetic resonance imaging (NMRI) was used to monitor changes in the state of bud water during the photoperiodic induction of endo-dormancy in Vitis riparia. Bud water status was monitored using proton relaxation times from T1 and T2 images determined at 2, 4, and 6 weeks of long (LD) or short (SD) photoperiod treatments. Bud dormancy was determined by monitoring budbreak in plants defoliated after photoperiod treatments. NMRI allowed nondestructive monitoring of changes in tissue water state. T1 and T2 maps indicated changes in the state of the water in bud and stem tissues during the 6 weeks of treatment. Differences in relaxation times for nondormant and dormancy-induced (reversible) buds were not clear. However, T2 relaxation times were lower in the dormant buds than in the nondormant buds.
Rajeev Arora and Lisa J. Rowland
To survive winters, woody perennials of temperate zone must enter into endodormancy. Resuming spring growth requires sufficient exposure to low temperature or chill units (CUs) in winter, referred to as chilling requirement (CR), which also plays a role in the development of freezing tolerance (cold acclimation; CA). Physiological studies on the breaking of dormancy have focused on identifying markers, such as appearance or disappearance of proteins in response to varying degrees of CU accumulation. However, whether these changes are associated with breaking dormancy or CA is not clear. We conducted a study, using greenhouse blueberry (Vaccinium section Cyanococcus) plants, to address this question Three blueberry cultivars (`Bluecrop', `Tifblue', and `Gulfcoast'), having CRs of ≈1200, 600, and 400 CUs, respectively, first were exposed to 4° for long enough to provide CUs equivalent to one-half of their respective CRs. This treatment resulted in CA. Plants were then transferred to 15C for 2 weeks (a treatment which should not negate CU accumulation but did result in deacclimation). Before and after each treatment cold hardiness (using a controlled freezing bath) and dormancy status (observe budbreak after placing shoots in water at 20C for 2 to 3 weeks) of floral buds were determined. Proteins were extracted from buds collected, simultaneously and separated by SDS-PAGE. To determine the association of dehydrin-like proteins with dormancy or CA, electroblots were probed with anti-dehydrin antibody. The relationship of protein and western blots data to cold acclimation and dormancy are presented.
Hongwen Huang, Desmond R. Layne and Thomas L. Kubisiak
Kentucky State Univ. (KSU) is the national clonal germplasm repository for Asimina species. Previous evaluation of the KSU pawpaw collection using 24 isozyme markers demonstrated that pawpaw has a relatively higher genetic diversity than that noted for other plant species with similar species characteristics (long-lived, woody, perennial, out-crossing, temperate, widespread, etc.). Current evaluation using RAPD markers will provide us with a more-accurate insight into pawpaw genetic diversity and population structure. In a preliminary experiment, one hundred 10-mer primers (OA1-20 through OE1-20, Operon Technologies Inc.) were screened against 32 commercial cultivars or advanced selections. A subset of 24 primers that amplify only the most-informative markers were used for germplasm evaluation. Sixty-eight RAPD markers were identified and used for determining genetic parameters. One-hundred-twenty pawpaw accessions were sampled from the KSU repository for RAPD analysis. These accessions represented nine widely distributed states within pawpaw's native range. RAPD data were subjected to various analyses using the NTSYS-PC computer program (ver. 1.8). Information generated from isozyme and RAPD markers will be used to formulate future germplasm collection strategies from wild populations within the native range. The implications of such information to the genetic enhancement of our repository and establishment of a core collection will be discussed.