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Jacqueline K. Burns, Louise Ferguson, Kitren Glozer, William H. Krueger and Richard C. Rosecrance

the olive by 2-chloroethanephosphonic acid and cycloheximide J. Amer. Soc. Hort. Sci. 95 635 640 Hartmond, U. Yuan, R. Burns, J.K. Grant, A. Kender, W.J. 2000 Citrus fruit abscission

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Shujun Zhou, Xin Tan, Liqin Fang, Jia Jian, Ping Xu and Guoliang Yuan

preparation. The protocol was according to Zhou et al. (2011) . When lily roots were ≈1 cm long, they were cut off and pretreated with 0.7 m m cycloheximide (Amresco, Solon, OH), at room temperature for 4 h, and then fixed in 3 ethanol:1 acetic acid

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Brian M. Schwartz, Ryan N. Contreras, Karen R. Harris-Shultz, Douglas L. Heckart, Jason B. Peake and Paul L. Raymer

24 h pretreatment in 3 m m 8-hydroxyquinoline (Fisher Scientific, Suwanee, GA) and 0.24 m m cycloheximide (Acros Organics, Morris Plans, NJ) at 4 °C, roots were fixed in Carnoy’s solution [100% ethanol, chloroform, and glacial acetic acid (6:3:1 v

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Shujun Zhou, Guixue Zhou and Kehu Li

. (2008) with minor modifications. When lily roots were ≈1 cm long, they were cut off and pretreated with 0.7 m m cycloheximide (Amresco) at room temperature for 4 h and then fixed in ethanol: acetic acid (Sinopharm Chemical Reagent Co., Ltd.) (3

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Kimberly Shearer and Thomas G. Ranney

seedlings and rooted stem cuttings in the mornings before 1000 hr . Root tips were suspended in a pre-fixative solution of 2 m m 8-hydroxyquinoline + 0.24 m m cycloheximide in glass vials and stored in the dark at room temperature for 3 h. Root tips were

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Robert C. Ebel, Jacqueline K. Burns, Kelly T. Morgan and Fritz Roka

Wilson, W.C. 1973 A comparison of cycloheximide with a new abscission chemical Florida State Hort. Soc. 86 56 60 Yuan, R. Alferez, F. Kostenyuk, I. Singh, S. Syvertsen, J.P. Burns

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Thomas G. Ranney, Tracy H. Thomasson, Kristin Neill, Nathan P. Lynch and Mark Weathington

-grown plants in mid-July and placed into a solution of 2 m m 8-hydroxyquinoline and 0.248 m m cycloheximide and incubated for 3 h at 23 °C, then moved to 4 °C for an additional 3 h. Roots were then rinsed in cold distilled water and fixed in Carnoy’s solution

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Thomas G. Ranney, Connor F. Ryan, Lauren E. Deans and Nathan P. Lynch

., 2007 ; Oh et al., 2003 ) to verify chromosome numbers and to calibrate genomes size measurements with ploidy levels. Actively growing root tips were excised and placed in a mixture of 4 m m 8-hydroxyquinoline and 0.249 m m cycloheximide for 3 h at

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Ryan N. Contreras, John M. Ruter and Brian M. Schwartz

Scientific, Suwanee, GA) + 0.24 m m cycloheximide (Acros Organics, Morris Plains, NJ) at 4 °C. After pretreatment, roots were transferred to Carnoy's solution [6 100% ethyl alcohol:3 chloroform:1 glacial acetic acid (by volume)] and fixed overnight at 25 °C

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Monica L. Elliott, J.A. McInroy, K. Xiong, J.H. Kim, H.D. Skipper and E.A. Guertal

aerobic bacteria and selection of bacteria for identification with gas chromatography for analysis of fatty acid methyl ester profiles (GC-FAME), solidified 10% tryptic soy broth (10% TSBA), amended with 100 μg·mL −1 cycloheximide to inhibit fungi, was