polysaccharide synthesis and metabolic energy ( Kwack, 1965 ). Alternatively, polyethylene glycol (PEG) has been used in some systems to lower the water potential of the media and allow sucrose concentrations to be lowered, producing higher germination rates and
at 20 °C/30 °C. For stress treatments, 60-d-old seedlings with uniform growth were removed from sand carefully and transferred in 1/2 Hoagland nutrient solution containing 20% PEG 6000 (dehydration stress) and 600 mmol·L −1 NaCl (salinity stress
cultured in half strength Hoagland solution with 30% of PEG-8000 (previously named PEG-6000; Sigma-Aldrich, St. Louis) (ψ o ≈ −1.2 MPa), while R 1 and R 2 were kept in half strength Hoagland solution only, and was denoted as R 3 P R 2 R 1 . The second
Thanks to Christina Gigliotti, Peg Pecora, and Kathy Anderson for their assistance with this project.
environments ( Springer and Goldman, 2016 ). Of the numerous osmotica available (e.g., PEG, mannitol, sucrose, and sodium chloride), PEG has been identified as most effective because it is nontoxic and has large molecules excluded from the seedcoat pores ( Hohl
. Two hundred fifty milligrams of seeds of one cultivar were placed in one cell. Each plate was floated in PEG-6000 (VWR International, Batavia, IL) solutions at 0.0 MPa [0% PEG-6000 (control, nonstressed)], −0.3 MPa (15% PEG-6000), or −0.6 MPa (22
Improved emergence rate and final stand occurred when ‘Ruby Queen’ beet seed pellets, amended with 1.10 to 3.95 mg polyethylene glycol 8000 (PEG) per seedball, were field planted. The number of seedlings per seedball 17 days after planting ranged from 1.39 to 1.60 for PEG-amended pellets, compared to 0.71 plants for non-PEG pellets or dry seeds. The PEG-amended seed pellets yielded 16 to 18 marketable roots per meter of row compared to 11 roots from non-PEG pellets.
`Verina' leek (AIlium porrum L.) seed germination is normally reduced at temperatures > 25C. Leek seeds were primed in aerated solutions (1.5 MPa, 10 days at 15C) of d-mannitol (mannitol), polyethylene glycol-8000 (PEG), KNO, and a nonaerated solution of PEG-8000 (PEG). At high temperatures mannitol, PEG, and PEG significantly enhanced germination percentage relative to KNO, or the control. At constant 30C, the mannitol, PEG, and PEG treatments increased final germination almost 10 times and the coefficient of velocity (COV) was improved compared to KNO, and the control. 10 growth chambers with alternating day/night temperatures (38 to 28C or 32 to 22C, 10 to 14 hours, respectively), primed seeds had significantly higher emergence and a larger COV than the control. In a greenhouse study under good conditions for germination, total emergence of primed and nonprimed seeds was similar; however, mannitol, PEG, and PEG led to a significantly higher COV than the control or KNO, treatments. These controlled-environment results demonstrate that priming leek seeds via mannitol, PEG, and PEG may promote early emergence at high temperature and improve stand uniformity for container transplant production.
retardants such as ancymidol ( El-Dawayati et al, 2012 ; Sarkar et al., 2001 ), and osmoregulators as polyethylene glycol (PEG-800) ( Seesangboon et al., 2018 ). In plant physiology, ancymidol produces a number of effects, including the inhibition of
Osmoconditioning carrot seed with polyethylene glycol (PEG) (Brocklehurst and Dearman, 1983) has accelerated germination, promoted seedling emergence in cold soil (5C) (Szafirowska et al., 1981), and also has reduced germination (Hegarty, 1977). The current preliminary study measured the influence of PEG on seedling emergence of carrot cultivar Candy Pack grown for seed production.