in storage roots of Asparagus officinalis L New Phytol. 120 463 473 Fujikawa, S. Jitsuyama, Y. Kuroda, K. 1999 Determination of the role of cold acclimation-induced diverse changes in plant cells from the viewpoint of avoidance of freezing injury J
Transient expression of electroporation-mediated DNA uptake was monitored in callus-derived protoplasts of two asparagus (Asparagus offcinalis L.) genotypes by measuring the GUS activity. The level of expression and the viability of the protoplasts were influenced by the voltage and duration of the electric pulse. An increased plasmid DNA concentration and the presence of polyethylene glycol (PEG) in the electroporation medium enhanced the transient expression level. A considerable increase in GUS activity was observed in the presence of both PEG and heat-shock treatments than with PEG treatment alone. An optimal level of GUS activity was obtained after electroporation with a capacitive discharge of 500 V/cm and 94 ms duration. The two genotypes differed in their responses in vitro and also showed variable levels of transient expression. The present technique was suitable to obtain transgenic plants, as histochemical GUS assay revealed GUS activity in the protoplast-derived microcolonies as well as in callus tissues.
Abstract
Tetraploid asparagus seedlings were produced from diploid seeds by means of colchicine treatment. Stomata length and cladophyll width were reliable characters for identifying diploids and tetraploids, but number of cladophylls per whorl and cladophyll length were unreliable.
Abstract
‘Jersey Centennial’ asparagus (Asparagus officinalis L.), tested under the designation Rutgers-Michigan Hybrid 202, is a new cultivar that is more vigorous, rust resistant, and productive than the ‘Mary Washington’ strain when grown on the fusarium-infested soils of New Jersey. It was also the most productive asparagus cultivar in a Michigan yield trial on noninfested soil.
Abstract
Asparagus (Asparagus officinalis L) was established in the field from 9 and 12-week-old greenhouse-grown seedlings and by direct seeding. Initial and over winter survival of transplants was not affected by age of seedling, method of growing, or the use of the an transparent Wilt-Pruf. Transplants produced more shoots, were taller and exhibited higher survival than seedlings established by direct seeding.
Abstract
‘Emerald’ asparagus (Asparagus officinalis L.) is a new cultivar well-adapted to northern states. It is intended for fresh market use, where the bright green spears provide an attractive alternative to typical asparagus showing anthocyanin at both spear base and tip. Quality appears to be equal to that of commonly used ‘Mary Washington’ and ‘Viking’, but winter survival and yield in young plantings have been superior.
In Japan, asparagus ( Asparagus officinalis L.) cropping types can be divided as follows: open field culture; semi-forcing culture; and rootstock planting forcing culture (RPFC) ( Motoki 2003 , 2016 ). With open field and semi-forcing cultures
-senescence removal of asparagus ( Asparagus officinalis L.) fern Acta Hort. 479 427 430 Bradford, M. 1976 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 248 254
Abstract
Factors determined to have a pronounced effect on shear-press peak-force values of asparagus, Asparagus officinalis L. included sample size, spear diameter, length of green, and preharvest temperature. Shear-press values were greater following periods of cold weather in early spring. Larger diameter spears had lower peak-force values than smaller spears, indicating that larger spears are more tender than smaller spears. The number and diameter of spears produced by a particular cultivar, and not differences between cultivars, were the characteristics observed to influence shear-press values. Shear-press max peak-force values correlated well with subjective and objective determinations of asparagus fiber.
Abstract
Sex influenced cladophylls/whorl, whorls, and leaf area/unit length of branch, primary and secondary branching, and stalk number and diameter in Asparagus officinalis L. cvs. UC72 and UC157 and in the parental clones of ‘UC157’. Staminate plants had more whorls per unit branch while pistillate plants had greater leaf area per cladophyll and per 10 cm of branch. The overall plant form of parental plants tends to appear in the progeny of the same sex in the UC 157 cultivar.