John R. Stommel, Judith A. Abbott and Robert A. Saftner
T. Casey Barickman, Dean A. Kopsell and Carl E. Sams
et al., 2013 ). The de novo synthesis of carotenoids in the tomato fruit tissue, mainly lycopene and β-carotene, are associated with the color changes from green to red as chloroplasts are transformed to chromoplasts ( Pék et al., 2010 ). Thus, the
Susan C. Miyasaka, Marisa Wall, Don LaBonte and Alton Arakaki
Sweetpotato is a nutritious source of food. Orange-fleshed cultivars are rich in β-carotene and purple-fleshed cultivars are rich in anthocyanins, both of which are important dietary antioxidants ( Teow et al., 2007 ; Wang et al., 2016 ). In the
Allan F. Brown, Gad G. Yousef, Ivette Guzman, Kranthi K. Chebrolu, Dennis J. Werner, Mike Parker, Ksenija Gasic and Penelope Perkins-Veazie
neochlorogenic acid), flavan 3-ols (catechin, epicatechin, procyanidins), flavonols (quercetin 3-glucoside and 3-rutinoside), and ANC (cyanidin 3-glucoside and 3-rutinoside) ( Tomás-Barberán et al., 2001 ). The carotenoid profile of peach includes β - carotene, β
Dean A. Kopsell and Carl E. Sams
regions (430 and 453 nm, respectively) of the visible light spectrum. In contrast, absorption of the carotenoid pigments of lutein (LUT) and β-carotene (BC) are highest in the blue region at 448 and 454 nm, respectively ( Lefsrud et al., 2008
Cecilia E. McGregor and Don R. LaBonte
`White Jewel' is a yellow-and-orange fleshed spontaneous mutant of the orange-flesh sweetpotato [Ipomoea batatas (L.) Lam.] cultivar Jewel. Mutations in storage root flesh color, and other traits are common in sweetpotato. The orange flesh color of sweetpotato is due to β-carotene stored in chromoplasts of root cells. β-carotene is important because of its role in human health. In an effort to elucidate biosynthesis and storage of β-carotene in sweetpotato roots, microarray analysis was used to investigate genes differentially expressed between `White Jewel' and `Jewel' storage roots. β-carotene content calculated from a* color values of `Jewel' and `White Jewel' were 20.66 mg/100 g fresh weight (FW) and 1.68 mg/100 g FW, respectively. Isopentenyl diphosphate isomerase (IPI) was down-regulated in `White Jewel', but farnesyl-diphosphate synthase (FPPS), geranylgeranyl diphosphate synthase (GGPS), and lycopene β-cyclase (LCY-b) were not differentially expressed. Several genes associated with chloroplasts were differentially expressed, indicating probable differences in chromoplast development of `White Jewel' and `Jewel'. Sucrose Synthase was down-regulated in `White Jewel' and fructose and glucose levels in `White Jewel' were lower than in `Jewel' while sucrose levels were higher in `White Jewel'. No differences were observed between dry weight or alcohol insoluble solids of the two cultivars. This study represents the first effort to elucidate β-carotene synthesis and storage in sweetpotato through large-scale gene expression analysis.
Dean A. Kopsell, Carl E. Sams, T. Casey Barickman and Robert C. Morrow
blue wavelengths (455 to 470 nm) significantly increased sprouting broccoli ( Brassica oleacea var. italica ) microgreen shoot tissue β-carotene, violaxanthin, total xanthophyll cycle pigments, glucoraphanin, epiprogoitrin, aliphatic glucosinolates
Kenneth R. Tourjee, Diane M. Barrett, Marisa V. Romero and Thomas M. Gradziel
The variability in fresh and processed fruit flesh color of six clingstone processing peach [Prunus persica (L.) Batsch] genotypes was measured using CIELAB color variables. The genotypes were selected based on the relative fruit concentrations of β-carotene and β-cryptoxanthin. Significant (p < 0.0001) differences were found among the genotypes for the L*, a*, and b* color variables of fresh and processed fruit. Mean color change during processing, as measured by ΔELAB, was greatest for `Ross' and least for `Hesse'. A plot of the first two principal components (PCs) obtained from PC analysis of the L*, a*, and b* variables for fresh and processed fruit revealed three clusters of genotypes that match groupings based on the relative concentrations in fresh fruit of carotenoid pigments. Path analysis showed that variation in β-cryptoxanthin concentration was more precisely determined from color data than β-carotene concentration. Chemical names used: β-β-carotene (β-carotene), (3R)-β-β-caroten-3-ol (β-cryptoxanthin).
Mark G. Lefsrud and Dean A. Kopsell
Controlled plant growing systems have consistently used the standard earth day as the radiation cycle for plant growth. However, the radiation cycle can be controlled using automated systems to regulate the exact amount of time plants are exposed to irradiation (and darkness). This experiment investigated the influence of different radiation cycle periods on plant growth and carotenoid accumulation in kale (Brassica oleracea L. var. acephala DC.). Plants were grown in a controlled environment using nutrient solutions under radiation cycle treatments of 2, 12, 24 and 48 hours, with 50% irradiance and 50% darkness during each cycle. The radiation cycles significantly affected kale fresh weight, dry weight, percent dry matter, and the accumulation of lutein, β-carotene, and chlorophyll a and b. Maximum fresh weight occurred under the 2-hour radiation cycle treatment, whereas maximum dry weight occurred under the 12-hour treatment. Maximum accumulation of lutein, β-carotene, and chlorophyll a occurred with the 12-hour radiation cycle at values of 14.5 mg/100 g, 13.1 mg/100 g, and 263.3 mg/100 g fresh weight respectively. Maximum fresh weight production of the kale was not linked to increases in chlorophyll, lutein, or β-carotene. Consumption of fruit and vegetable crops rich in lutein and β-carotene carotenoids is associated with reduced risk of cancers and aging eye diseases. Increased carotenoid concentrations in vegetable crops would therefore be expected to increase the value of these crops.