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% trichloroacetic acid. The absorbance of the supernatant was measured at 280 nm. Polyphenol oxidase activity assay. The polyphenol oxidase (PPO) assay was performed in a mixture containing 2.85 mL of 50 m m PBS (pH 7.0), 50 µL of 60 m m catechol, and 0.1 m m of
Mycobacterium tuberculosis complex by modified comparative genomic analysis Infect Genet Evol. 70 158 – 164 . https://doi.org/10.1016/j.meegid.2019.01.012 Kanjanapongkul K Baibua V. 2021 Effects of ohmic pasteurization of coconut water on polyphenol oxidase
.4.1341 Barbagallo, R.N. Chisari, M. Branca, F. Spagna, G. 2008 Pectin methylesterase, polyphenol oxidase and physicochemical properties of typical long-storage cherry tomatoes cultivated under water stress regime J. Sci. Food Agr
(H 2 S) on the activities of ( A ) catalase (CAT); ( B ) ascorbate peroxidase (APX); ( C ) guaiacol peroxidase (POD); ( D ) superoxide dismutase (SOD); ( E ) glutathione reductase (GR); ( F ) lipoxygenase (LOX); ( G ) polyphenol oxidase (PPO); ( H
aspirin, tea polyphenols, and retinoic acid, have been reported to inhibit cell transformation and tumor promotion by suppressing AP-1 transactivation ( Agadir et al., 1999 ; Dong et al., 1997a , b ). Nuclear factor-κB is a heterodimeric protein
) and Zipor et al. (2015) argued that peroxidase requires H 2 O 2 and that polyphenol oxidase requires O 2 for the anthocyanin degradation reaction. Furthermore, Barbagallo et al. (2007) showed that β-glucosidase hydrolyzes anthocyanin. However
functions in stress tolerance. Two enzymes were only induced in LA3465, which include aldehyde dehydrogenase in detoxifying peroxidic aldehydes, which result from lipid peroxidation, and polyphenol oxidase. Several studies on tomato have shown that an
zero, and the actual reading corresponded to the absorbance after 1 min. One unit of CAT activity was defined as a 0.01 decrease in absorbance at 240 nm/mg of protein/min. Polyphenol oxidase assay. Polyphenol oxidase (PPO; EC 1.10.3.1) activity was
Roberts, 2004 ; Lee et al., 2002 ). In addition to increased juice recovery, the elevated temperature also denatures native enzymes, such as polyphenol oxidase, that can otherwise cause degradation of flavor, color, and phytochemical levels ( Patras et al
), and thioredoxin (2.18-fold) were induced only in S. chilense LA1958. Polyphenol oxidases (PPO) catalyze the reaction for the formation of cytotoxic orthoquinones. In the dehydration treated S. chilense LA1958, PPO was repressed (–1.93-fold), but