georgia plume have been made, yet conventional techniques have not been effective. Propagation by shoot cuttings has been unsuccessful, and root cuttings can produce only a limited number of shoots ( Fordham, 1991 ). Scanty population numbers and lack of
, distribution, and average length. Bud selection has been reported to promote lateral branching in central and multiple leader sweet cherry training systems ( Hoying et al., 2001 ) and, in this study, increased desirable shoot number and improved distribution of
buds and multipleshoots developed at all zeatin concentrations ( Fig. 1B ).
An interaction ( P ≤ 0.05) between zeatin concentration and subculture period was observed for shoot number, shoot height, and number of leaves per shoot ( Table 1 ). Zeatin
) multipleshoots in A. canadensis cotyledon culture; ( F ) multipleshoots in A. racemosus cotyledon culture; ( G ) calli derived from A. racemosus cotyledon culture; ( H ) small plantlets from A. racemosus callus subculture; ( I ) plantlet of A
treatments evaluated. Young buds induced an average of 11.2 shoots per explant, whereas mature buds induced 6.2 shoots per explant. These results suggest that bud position in the stem (physiological age) is a determining factor for adventitious multiple soot
present study were 1) to examine the response of intersectional hybrids between shashanbo and highbush blueberry ‘Spartan’ to different pH levels; and 2) to establish an in vitro screening system using multipleshoots to identify individuals that exhibit
effective for multiplication of clones, it is expensive and often generates somatic mutations ( Padmanabhan et al., 1974 ).
The complete decapitation method (CDM), which regenerates multipleshoots from the cut surfaces of the main and lateral stems of
accumulated in shoot tissues were related to turf quality ratings. Stepwise multiple regressions were done by using turf quality as a response variable and 13 mineral concentrations plus K/Na ratio as predictor variables, and significance was chosen at the
0.1 and 2 mg/L showed values ranging between 2.58 and 8.58, higher than the other media. ZR and 2-iP were not able to induce multipleshoot formation and, in both cases, the number of shoots per explants was lower than the control (hormone
patterns of chimera generation. To eliminate the chimeras, an efficient protocol was developed for purifying isogenic transformants through multiple rounds of shoot regeneration processes. Based on the fact that the transgenes were inherited to the second