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Ya-Long Qin, Xiao-Chun Shu, Wei-Bing Zhuang, Feng Peng and Zhong Wang

resistant and high yielding, can be imported into the cultivated plant to obtain high-quality varieties through genetic engineering ( Jin et al., 2004 ). Regeneration systems have been successfully established in many varieties of Solanum L., including

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Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano and Zong-Ming (Max) Cheng

breeding ( Portillo et al., 2007 ). Therefore, the most effective means for genetic improvement is through biotechnology. Micropropagation systems and regeneration systems through either organogenesis or somatic embryogenesis have already been established

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Rochelle R. Beasley and Paula M. Pijut

, inadequate natural regeneration, and various interacting (climatic, edaphic, and physiographic) factors ( Benedict and David, 2000 ; Palik et al., 2011 , 2012 ; Ward et al., 2006 ). The emerald ash borer (EAB; Agrilus planipennis ), an aggressive exotic

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Nevena Mitić, Mariana Stanišić, Jelena Milojević, Ljiljana Tubić, Tatjana Ćosić, Radomirka Nikolić, Slavica Ninković and Rade Miletić

in apple is the development of an efficient regeneration system that would increase adventitious shoot regeneration, because the regeneration ability of transformed tissues is much lower than that of non-transformed ones ( Pawlicki-Jullian et al

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Dongliang Qiu, Xiangying Wei, Shufang Fan, Dawei Jian and Jianjun Chen

existing meristems, whereas shoot organogenesis refers to regeneration from explants without preexisting meristems. The latter generally gives rise to a large number of shoots and is considered more efficient for plant multiplication. However, shoot

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Juanxu Liu, Min Deng, Richard J. Henny, Jianjun Chen and Jiahua Xie

; Blanco et al., 2004 ; Debergh, 1976 ) and D. fragrans ( Deberghs, 1975 , 1976 ; Debergh and Maene, 1981 ) and regeneration of stem explants of D. marginata ( Chua et al., 1981 ) and D. fragrans ( Lu, 2003 ; Vinterhalter and Vinterhalter, 1997

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Jin Cui, Juanxu Liu, Jianjun Chen and Richard J. Henny

Flash’ produced in 15-cm pots from liners regenerated using the indirect shoot organogenesis method established in this study, in which each pot has only one shoot and no distorted leaves. As a result of its unique color and adaptation to low light

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Takahiro Tezuka, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka and Masayuki Oda

’ developed in Fukui Prefecture ( Sato et al., 2009 ). However, the efficiency of vegetative propagation by traditional methods is insufficient for mass propagation of plantlets. Adventitious shoots can be regenerated from cut surfaces of hypocotyls or stems

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L. Xu, G.F. Liu and M.Z. Bao

inherited traits. In L. styraciflua , plants have been regenerated via adventitious shoots from mature leaf and petiole segments ( Brand and Lineberger, 1988 ) and hypocotyl segments ( Kim et al., 1997 ), and via somatic embryogenesis from hypocotyl

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S.L. Kitto and G. McGranahan

Cotyledons and embryonic axes of Pistacia vera L. `Kerman' were cultured on modified DKW or MS media. Cotyledonary petioles regenerated shoots when cultured on DKW medium supplemented with (in g/liter) 2.5 Zn(NO3)2, 0.48 H3BO3, 100 KNO3 and (in mg/liter) 1 BA, 0.01 IBA, 2 kinetin and 250 glutamine whether under light (80 μmol·m-2·s-1) or dark conditions. Cotyledons regenerated floral parts from their abaxial surfaces when cultured on (1) DKW or MS, both supplemented with (in mg/liter) 600 glutamine, 300 glycine, 200 asparagine, 50 arginine, 20 ascorbic acid and 0.05-0.1 2,4-D plus 0.1 BA or 0.1 zeatin under light or dark conditions or (2) DKW medium supplemented with (in g/liter) 2.5 Zn(NO3)2, 0.48 H3BO3, 100 KNO3 and (in mg/liter) 0.002 thidiazuron plus 0.2 2,4-D under light conditions.