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Roberto Valverde and Tony H. H. Chen

To generate a linkage map for further genetic analysis of the traits involved in cold hardiness of potato, we are constructing a RAPD-based linkage map using a two-generation interspecific pedigree of Solanum commersonii and S. cardiophyllum, a hardy and non-hardy species, respectively. We initially screened 220 primers of 10-base arbitrary sequences and selected 86 to amplify a total of 577 polymorphic bands: 301 S. commersonii-specific and 276 S. cardiophyllum-specific bands. Segregation of a total of 247 markers was scored on a population of 44 F1 individuals. From these 247 markers, we have identified 117 markers, which segregate 1:1 in the F1 progeny following a test cross configuration. A RAPD linkage map for S. commersonii will be presented.

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Sinchieh Liu and Martha A. Mutschler

The transfer of multigenic traits into tomato has been slow due to interspecific barriers (hybrid breakdown) found in the F2 of the Lycopersicon esculentum × L. pennellii cross (esc × pen), including blocks in normal reproductive development and nonfecundity. In a typical (esc × pen) F2 population, failure to flower and premeiotic blocks in pollen development occurred in 2% and 11% of the population, respectively. The remaining plants showed a mean of 37% stainable pollen. Twenty three percent of the F2 plants set seed, with an average of 4.5 seeds/fruit. An average of 33% of the stainable pollen from the 7 F2 plants with the highest stainable pollen measurements germinated in vitro, but only 4 of these 7 plants set seed. Thus, percent stainable pollen is not an adequate predictor of fecundity, and the non-fecundity in the F2Le plants must involve barriers occurring after pollen germination.

A method was developed which greatly reduces or eliminates each of the F2 barriers. The method and its efficacy on each of the aspects of hybrid breakdown will be discussed.

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Luping Qu and James F. Hancock

A tetraploid blueberry population resulting from a cross of US 75 {a tetraploid hybrid of Fla 4B [a selection of Vaccinium darrowi Camp (2n = 2x = 24) × `Bluecrop' [(V. corymbosum L. (2n = 4x = 48)]} × `Bluetta' (4x) was used to generate a genetic linkage map of US 75 by randomly amplified polymorphic DNA (RAPD) analysis. One hundred and forty markers unique for Fla 4B that segregated 1:1 in the population were mapped into 29 linkage groups that cover a total genetic distance of 1288.2 cM, with a range of 1.6 to 33.9 cM between adjacent markers. The map is essentially of V. darrowi because US 75 was produced via a 2n gamete from Fla 4B and only unique markers for Fla 4B were used. Therefore, all the chromosomes of V. darrowi could be represented in the map.

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J. Erron Haggard and James R. Myers

White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, causes major losses in dry and snap bean (Phaseolus vulgaris) production. With little genetic variation for white mold resistance in common bean, other potential sources for resistance must be investigated. Accessions of scarlet runner bean (P. coccineus) have been shown to have partial resistance exceeding any to be found in common bean. Resistance is quantitative with at least six QTL found in a P. coccineus intraspecific resistant × susceptible cross. Our goal is to transfer high levels of resistance from P. coccineus into commercially acceptable common bean lines. We developed interspecific advanced backcross populations for mapping and transfer of resistance QTL. 111 BC2F5 lines from a cross between OR91G and PI255956 have been tested in straw tests and oxalate tests, as well as in a field trial. The data show that the OR91G × PI255956 population carries a high level of resistance, but because of the quantitative nature of resistance, it may be necessary to intercross individuals to achieve higher levels. SSR, RAPD, and AFLP markers are being tested in the population to construct a linkage map for placement of QTL. QTL identified from each type of test (straw, oxalate, and field) may provide additional information about the genetic architecture of white mold resistance. Three other populations are from advanced backcrosses of the recurrent parents G122, OR91G, and MO162, with PI433251B as the donor parent in each. Analyses and advance of these populations will follow, the results of which should confirm QTL identified in the OR91G × PI255956 population, as well as possible additional resistance QTL from PI433251B.

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Mark J. Bassett

The genetics of the vermilion flower color (more orange than scarlet or salmon red) of Phaseolus coccineus L. is largely unknown, but the gene Sal for salmon red is the gene essential for its expression. Lamprecht line M0169 (PI 527868) expresses salmon red flowers with vein pattern on the wing petals and black seedcoats. M0169 (Sal Am and an unknown gene that inhibits the scarlet flower color expression of Am) was crossed with v BC3 5-593 (sal am and no inhibitor gene, expressing white flowers and mineral brown seedcoats). Line 5-593 is a Florida dry bean (Phaseolus vulgaris L.) line used as the recurrent parent for development of genetic stocks. The F2 from Sal Am V wf BC1 5-593 (scarlet flowers, black seedcoats) × v BC3 5-593 (white flowers, mineral brown seedcoats) supported the hypothesis that a partly dominant gene Am changes salmon red to scarlet flower color and that Am has no expression with sal. The F3 progeny test of 27 random F2 parents from the above cross supported the hypothesis of a single partly dominant factor (Am) with no expression without Sal, where only Sal/Sal Am/Am completely eliminates the flower vein pattern (VP) of Sal. F4 progeny tests of 29 random F3 parents derived from a F2 selection with Sal/Sal Am/am V wf/v supported the hypothesis that Am is linked to V (cM = 9.4 ± 1.93) and the hypothesis that Am is linked with a dominant gene (tentative symbol Oxb) that (with Sal v) changes seedcoat color from mineral brown with red haze to oxblood red. Another F4 progeny test of seven selected F3 parents with Sal/Sal Am/am v/v and oxblood seedcoat color supported the hypothesis that the Oxb gene (linked with Am and derived from M0169) with Sal v expresses oxblood seedcoat color. The gene symbol Am is proposed for the gene from M0169 that with Sal v expresses two pleiotropic effects: changes salmon red to scarlet flower color and eliminates the VP of salmon red. The interaction of Sal with Am for flower color and VP expression is discussed for all gene combinations.

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Hongwen Huang, Fenny Dane and Joseph D. Norton

Allozyme polymorphism in chestnut (Castanea) species was investigated using isoelectric focusing in thin-layer polyacrylamide slab gels. Genetic analysis of the progenies of intraspecific crosses and interspecific F2s and backcrosses (BC1s) allowed the verification of 11 polymorphic isozyme loci from 11 enzyme systems. The following loci were defined: Acp, Adh, Est-1, Est-2, Est-5, Me, Prx-1, Prx-2, Prx-3, Skd-3, and Skd-4. All polymorphic loci behaved as single-locus Mendelian genes. Skd showed unique species specificity. Skd-1 and Skd-2 were unique to the American chestnut (C. dentata Borkh.) and the European chestnut (C. sativa Mill.), whereas Skd-3 and Skd-4 were unique to the Chinese chestnut (C. mollissima Bl.) and the Japanese chestnut (C. crenata Sieb.). Linkage analysis revealed linkage for three pairs of loci: Skd-3/Skd-4, Est-1/Est-2, and Est-5/Prx-1. The single-tree progeny method was used successfully for isozyme genetic analysis. Forty-seven chestnut cultivars in six chestnut species were characterized using 12 isozyme loci and can be unambiguously identified by 12 multi-locus genotypes. The interspecific and geographic relationships among species were also discussed.

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Susan M. Hawkins, John M. Ruter and Carol D. Robacker

these crosses was counted. Results Pollen tubes were able to grow to the end of the style in every cross tested ( Table 2 ; Figs. 1 and 2 ). Intergeneric crosses and interspecific crosses showed the same pattern, indicating that prezygotic barriers to

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Qi Zhang, Enda Yu and Andy Medina

annual species and can be crossed with difficulty with C. maxima , C. pepo , and C. mixta . Fertile seeds from a series of interspecific crosses were successfully obtained in the past few decades ( Baggett, 1979 ; Castetter, 1930 ; Erwin and Haber

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Irene E. Palmer, Thomas G. Ranney, Nathan P. Lynch and Richard E. Bir

2006, 2007, and 2008 using standard horticultural practices. Forty-three interspecific crosses were completed in a greenhouse with at least four pollinated inflorescences per cross. The greenhouse was screened to exclude insect pollinators. All