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Ariadna Monroy-Barbosa and Paul W. Bosland

infection of pepper fruit in various physical environments Proc. Okla. Acad. Sci. 75 1 5 Bosland, P.W. Lindsey, D.L. 1991 A seedling screen for Phytophthora root rot of pepper, Capsicum annuum Plant Dis. 75 1048

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Baniekal Hiremath Gangadhar, Raghvendra Kumar Mishra, Gobinath Pandian and Se Won Park

. The LEDs consisting of red, blue, and red plus blue LEDs along with fluorescent light had shown a significant influence on growth and morphology of Capsicum annuum cv. Cheonyang plants. Different plant growth parameters like plant height, leaf area

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Chae Shin Lim, Seong Mo Kang, Jeoung Lai Cho, Kenneth C. Gross and Allan B. Woolf

Sweet cultivars of Capsicum annuum L. are widely cultivated throughout the world because of their nutritional value, flavor, and color. However, bell peppers are susceptible to chilling injury (CI) below 7 °C ( Paull, 1990 ) depending on

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Zheng Wang, Mark Williams, Krista Jacobsen and Timothy Coolong

controlling soil moisture-based irrigation in field-grown vegetables HortScience 48 S45 abstr. Diaz-Perez, J.C. 2010 Bell pepper ( Capsicum annuum L.) grown on plastic film mulches: Effects on crop microenvironment, physiological attributes, and fruit yield

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Lucianne Braga Oliveira Vilarinho, Derly Jose Henriques da Silva, Ann Greene, Kara Denee Salazar, Cristiane Alves, Molly Eveleth, Ben Nichols, Sana Tehseen, Joseph Kalil Khoury Jr., Jodie V. Johnson, Steven A. Sargent and Bala Rathinasabapathi

Capsicum annuum , a vegetable crop of international importance, has rich genetic diversity for fruit traits. Much of this diversity has been exploited to develop sweet, blocky peppers that are most popular in commercial production and typically

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M.K. Riley and P.W. Bosland

Two studies were performed to evaluate techniques for screening verticillium wilt of Capsicum annuum L. The first study tested inoculation methods. The original method involved mixing the inoculum with planting medium in a cement mixer for 1 h. Seeds then were planted in the infested medium. In the new technique, inoculum is poured directly into the row, and seeds are placed directly on top of the inoculum. Inoculum levels of 2000 and 1000 mcrosclerotia/g of soil were tested in the new “in-row” method. The disease severity of the “in-row” plants was significantly less than the plants inoculated by the original method. A significant difference remained between resistant and susceptible lines. There was no difference between inoculum levels. The second study compared three commercial planting media to the standard soil used in previous screenings. Disease severity did not differ among media, and all media showed significant differences between resistant and susceptible C. annuum lines.

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Cláudia S. da Costa Ribeiro and Paul W. Bosland

identify physiological races of P. capsici for phytophthora root rot and phytophthora foliar blight syndromes in pepper using a set of different cultivars of Capsicum annuum as host differentials. Phytophthora capsici is a heterothallic species with

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Rhiana F. Jones, Paul W. Bosland, Robert L. Steiner, Richard W. Jones and Mary A. O’Connell

capsici (race PWB24) inoculated leaves in Capsicum annuum at 4, 24, and 72 h. ( A ) resistant landrace Criollo de Morelos 334 (CM334); ( B ) susceptible cultivar NMH6-4. Gene expression changes in response to P. capsici challenge. Factorial ANOVA and LS

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Arnon Ben-Chaim and Ilan Paran

Inheritance of 10 quantitative traits related to plant and fruit development was studied in an intraspecific cross between a bell-type `Maor' [Capsicum annuum L. var. annuum (Grossum Group) `Maor'] and a small-fruited pungent chilli line `Perennial' [C. annuum var. annuum (Longum Group) `Perennial']. Estimates of broad- and narrow-sense heritabilities, coefficients of genetic variance, and genotypic correlations were obtained from the segregation of 120 F3 families in 2 years. Three of the traits analyzed, days to first ripened fruit, plant height, and pedicel length, exhibited heterosis and transgressive segregation. Days to first ripened fruit and total soluble solids had low narrow-sense heritabilities. The other traits studied had moderate to high narrow-sense heritability estimates. Most of the genetic variation associated with traits that affect the size of the fruit and its shape was additive. The highest genetic correlation coefficients among pairs of traits were found between fruit weight and each of the three width characters: fruit diameter, pericarp thickness, and pedicel diameter. In contrast, fruit weight had a low correlation coefficient with fruit length, indicating that the size of the pepper fruit in this cross was determined primarily by its width.

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S. Jayashankar, Suman Bagga and Gregory C. Phillips

In vitro genetic transformation of chile pepper, Capsicum annuum var. New Mexico 6-4, was achieved. Seeds of `New Mexico 6-4' were grown aseptically on Murashige and Skoog medium. Seedlings 22 days old were wounded on the hypocotyl region using a sterile hypodermic needle. A. rhizogenes strain K 599 harboring the plasmid p35S GUS Intron was inoculated on the wound site. Three days later the seedlings were transferred onto MS media with antibiotics (Cefotaxime, Carbenicillin, Amoxicillin, Clavulanic acid, and Kanamycin). New roots were seen to initiate from the wound site 15 to 20 days after inoculation. The roots were morphologically identified as “hairy roots.” Glucuronidase (Gus) assay performed 40 days after inoculation on randomly chosen roots that had grown into the selection medium, showed that 6/25 (24%) of the inoculated seedlings had roots that showed intense blue coloration. Presence of an intron makes it impossible for the bacteria to express the reporter gene. The seedlings that had transformed roots had a different morphology with wrinkled leaves and short internodes. The pattern of expression of the introduced gene varied greatly. Some positive tissues had the root tips alone being blue; a few had the vascular tissues and the root tips blue; and others had the vascular tissues, the surrounding parenchyma cells, root tips, and the root hairs turn very dark blue. The transformed roots did not need to grow into the selection media to be Gus positive. Isolated roots cultured on MS media supplemented with 0.2 mg/L IAA were maintained for 120 days and continued to express the reporter gene. Currently, methods to regenerate transformed shoots from roots are being tested. The “hairy root” transformation system in pepper could have application in the testing of root-expressible constructs for transgene expression assays.