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Kadir Uğurtan Yılmaz, Sezai Ercişli, Bayram Murat Asma, Yıldız Doğan and Salih Kafkas

levels in the genus, Prunus , is limited to a few molecular phylogenetic studies using ITS and chloroplast trnL-trnF spacer sequence variation ( Bortiri et al., 2001 ) and amplified fragment length polymorphism ( Aradhya et al., 2004 ). Choice of the

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Xuejuan Chen, Ming Sun, Jianguo Liang, Hui Xue and Qixiang Zhang

′-TACTCAGGACTCAT-5′ ( Hill et al., 1996 ; Sun Ming, 2007 ). We used 280 pairs of EcoR I (E) and Mse I (M) primers to analyze our 74 accessions. Finally, 19 high polymorphism EcoR I+ Mse I primer combinations were used for this study ( Table 2 ). Preselective

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Salih Kafkas, Yıldız Doğan, Ali Sabır, Ali Turan and Hasbi Seker

amplified polymorphic DNA (RAPD) ( Galderisi et al., 1999 ; Miaja et al., 2001 ), amplified fragment length polymorphism (AFLP) ( Ferrari et al., 2004 ), and simple sequence repeat (SSR) ( Boccacci et al., 2006 ; 2008 ; Gokirmak et al., 2009 ). Palme and

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Jianfeng Liu, Bowen Yang, Yuetong Ming, Yuchu Zhang and Yunqing Cheng

desirable genetic attributes, including relative abundance, high polymorphism, codominant inheritance, transferability to related species and genera, and good genome coverage ( Guo et al., 2016 ). The expressed sequence tag (EST)-SSRs are widely used in the

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Karen R. Harris, Kai-Shu Ling, W. Patrick Wechter and Amnon Levi

quantification of virus replication by ELISA, the visual scoring for virus symptoms, and the extraction of DNA from plants were as described previously ( Ling et al., 2008 ). Screening markers for polymorphism and for linkage with the ZYMV-FL resistance

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Jun-Bo Yang, Hong-Tao Li, De-Zhu Li, Jie Liu, Lian-Ming Gao, De-Zhu Li, Lian-Ming Gao and Jie Liu

. In previous studies, random amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) data ( Mohapatra et al., 2008 , 2009 ; Saikia et al., 2000 ; Zhang et al., 2009 ) have been used to

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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe and Sheri B. Crabtree

minisatellite probe ( Rogstad et al., 1991 ), allozymes ( Huang et al., 1997 , 1998 ), random amplified polymorphic DNA [RAPD ( Huang et al., 2000 , 2003 )], amplified fragment length polymorphism [AFLP ( Wang et al., 2005 )], intersimple sequence repeat [ISSR

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Allan F. Brown, Elizabeth H. Jeffery and John A. Juvik

existing elite germplasm. A number of PCR-based molecular marker systems have been developed in recent years that share a number of common advantages over RFLPs: they are more readily amenable to automation, often produce more polymorphisms per reaction

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Jose A. Oliveira, Ana B. Monteagudo, Suleiman S. Bughrara, Jose L. Martínez, Ana Salas, Esther Novo-Uzal and Federico Pomar

information obtained can be used to determine ploidy levels ( Arumuganathan and Earle, 1991 ). Amplified fragment length polymorphism (AFLP) markers have been used successfully to determine the genetic diversity of forage and turfgrasses ( Zhao et al., 2006

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Nader R. Abdelsalam, Hayssam M. Ali, Mohamed Z.M. Salem, Elsayed G. Ibrahem and Mohamed S. Elshikh

)–polymerase chain reaction (PCR) ( Rajwana et al., 2008 ), restricted fragment length polymorphism, and amplified fragment length polymorphism (AFLP) ( Yamanaka et al., 2006 ) have been used to assess the genetic diversity among mango genotypes/cultivars. Recently