into ≈0.5-cm long sections and incubated on MSI medium containing 0, 2.22, 4.44, 8.88, 13.32 μM BA in all combinations with 0, 0.54, 2.68, 5.36 μM NAA. Leaf segments were placed in petri dishes (1.5 × 8.5 cm) containing 20 mL medium. Explants were
of S. rebaudiana , explants were cultured on WPM supplemented with different PGRs. WPM supplemented with BA in combination with NAA gave the best response regarding shoot proliferation. The highest number of shoots (10.02 shoots/shoot tip explant
supplemented with 0.0, 1.0, 2.0, 4.0, or 8.0 μ m dicamba, picloram, K-NAA, or 2,4-D. The PGRs and concentrations were selected based on previous reports of their use for other Lilium taxa ( Famelaer et al., 1996 ; Haensch, 1996 ; Okazaki and Koizumi, 1995
-phenyl-N′-1,2,3-thiadiazol-5-ylurea (TDZ) with 1.07 μ m α-naphthalene acetic acid (NAA) or with 2.26 μ m 2,4-dichlorophenoxyacetic acid (2,4-D), which were based on the formula of Zhang et al. (2006) . Additionally, the test also included 8.88 μ m 6
length of the first flower of lily. The optimum concentrations of NAA and 6-BA are important for in vitro propagation of lily. BA promoted the formation of bulblets on scales and stem elongation in bulblets formed on scales of L. formolongi ( Ishimori
acetic acid (NAA), and 6-furfurylamino purine [kinetin (KT)], are commonly used by most researchers for micropropagation ( Panda et al., 2007 ). BAP is the most preferred PGRs for shoot elongation and multiplication, and NAA is generally known to promote
calluses from phytoplasma-infected stem tissues. These callus tissues differentiated into plants on the MS medium with 0.25 mg·L −1 6-benzyladeine (BA), 1.0 mg·L −1 1-naphthalene acetic acid (NAA), and 10.0 mg·L −1 gentamicin. Further test confirmed that
and solidified with 2.0 g·L −1 gelrite. Different concentrations of BA (0, 1, 3, 5, and 7 mg·L −1 ), Kin (0, 1, 3, 5, and 7 mg·L −1 ), TDZ (0, 0.5, 1.0, 1.5, and 2.0 mg·L −1 ), NAA (0, 0.5, and 1.0 mg·L −1 ), and IBA (0, 0.5, and 1.0 mg·L −1 ) were
top in Feb. 2014 were trimmed to 12 cm long. Auxin [1-naphthalene acetic acid (NAA), indole 3-butyric acid (IBA), and indole 3-acetic acid (IAA)] powders (Sigma-Aldrich, Shanghai, China) were dissolved in a small amount of ethanol (analytical reagent
(ZT), or 0.54, 5.4, 10.8, or 21.6 μM NAA for callus induction. WPM without any plant growth regulator (PGR) was prepared as a control medium. Media containing 0.45, 4.5, or 9.0 μM BA in combination with 4.5 μM 2,4-D or 5.4 μM NAA were further evaluated