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Jack E. Staub, Zhanyong Sun, Sang-Min Chung and Richard L. Lower

), sequenced characterized amplified regions (SCAR), and amplified fragment length polymorphism (AFLP)], as well as codominant [simple sequence repeat (SSR), single nucleotide polymorphism (SNP)] markers have been used extensively in these maps for the

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Margaret T. Mmbaga, and Roger J. Sauvé,

., 1995 ; Weising et al., 1995 ; Welsh and McClelland, 1990 ; Zhou and Sauvé, 2002 , 2006 ). The amplified fragment length polymorphism (AFLP) technique is one of the most reliable methods for genomic analysis ( Jiang et al., 2000 ; Vos et al., 1995

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Michael Dossett, Jill M. Bushakra, Barbara Gilmore, Carol A. Koch, Chaim Kempler, Chad E. Finn and Nahla V. Bassil

generate single nucleotide polymorphism (SNP) markers, or to use amplified fragment length polymorphisms (AFLPs) or other marker systems to develop linkage maps, the development of markers, such as SSRs, that are locus-specific is an ongoing objective in

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Joseph C. Kuhl and Veronica L. DeBoer

selective amplification of restriction fragments ( Vos et al., 1995 ). Amplified fragment length polymorphism (AFLP) analysis does not require prior knowledge of an organism's genome ( Vos et al., 1995 ) and has been widely shown to be reproducible ( Jones

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Zhuang-Zhuang Liu, Tao Chen, Fang-Ren Peng, You-Wang Liang, Peng-Peng Tan, Zheng-Hai Mo, Fan Cao, Yang-Juan Shang, Rui Zhang and Yong-Rong Li

methylation-sensitive amplified polymorphism (MSAP) method has been used by numerous researchers. MSAP, based on amplified fragment length polymorphism, was introduced by Reyna-López et al. (1997) , wherein Mse I was replaced by two isoschizomers (HpaII and

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Chunxian Chen and William R. Okie

known amplification reliability and polymorphism status are needed to facilitate maternal inheritance and phylogenetic studies on peach and other Prunus species. Several chloroplast genome sequences from Prunus species are available in the GenBank

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Luwbia Aranda, Timothy G. Porch, Mark J. Bassett, Laura Lara and Perry B. Cregan

pattern and color genes in common bean, viz., T , Bip , C , Z , J , G , V , and Gy ( Bassett et al., 1999 , 2002a , 2002b ; Bassett and McClean, 2000 ; Brady et al., 1998 ; McClean et al., 2002 ). In these studies, the polymorphisms were

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Raúl De la Rosa, Angjelina Belaj, Antonio Muñoz-Mérida, Oswaldo Trelles, Inmaculada Ortíz-Martín, Juan José González-Plaza, Victoriano Valpuesta and Carmen R. Beuzón

identified using Sanger technology. The purpose of the present work is to develop EST-derived hexa-nucleotide SSR markers and to evaluate their polymorphism and use for paternity testing. Materials and Methods EST-SSR mining and primer design. In the

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Jiang-Chong Wu, Jing Yang, Zhi-Jian Gu and Yan-Ping Zhang

efficient molecular markers for M. oleifera is needed. Based on codominant features and high allelic polymorphism, microsatellites [simple sequence repeats (SSRs)] have become a useful marker system in genetic diversity studies ( Walter and Epperson

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Zhanao Deng, Fahrettin Goktepe, Brent K. Harbaugh and Jinguo Hu

al., 2006a ; Carr et al., 2003 ; Chen et al., 2004a , 2004b , 2004c ; Loh et al., 1999 , 2000 ; Meerow et al., 2005 ). The target-region amplification polymorphism (TRAP) system is a recent addition to these PCR-based systems ( Hu, 2006 ; Hu