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acidification of vacuoles and seed coat morphogenesis. They also showed that different functions of the an1 gene could be abolished by mutating different domains of the gene. Later work by Quattrocchio et al. (2006) showed that AN1 protein can have

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Chemical nature of a semipermeable layer in seed coats of leek, onion (Liliaceae), tomato and pepper (Solanaceae) Seed Sci. Technol. 23 135 145 Briggs, G. Bromilow, R. Evans, A. 1982 Relationships between lipophilicity and root uptake and translocation of

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dihydroflavond reductase (DFR) expression ( Spelt et al., 2002 ). When mutant Myc An1 alleles lacking the bHLH domain were overexpressed, near-normal levels of anthocyanin biosynthesis occur without vacuolar acidification or normal seed coat development

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leaves of the Ni hyperaccumulators Alyssum murale and Alyssum corsicum Plant Soil 314 35 48 Buss, C.C. Lammers, T.G. Wise, R.R. 2001 Seed coat morphology and its systematic implications in Cyanea and other genera of Lobeliodeaceae (Campanulaceae

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.D. Hummer, K.E. 2003 Control of powdery mildew on leaves and stems of gooseberries HortTechnology 13 365 367 Wada, S. Reed, B.M. 2010 Seed coat morphology differentiates blackberry cultivars Journal of the American Pomological Society 64 151 160 Williams, K

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( Glycine max ) row width and density of redroot pigweed ( Amaranthus retroflexus ) Weed Sci. 37 84 92 Mavi, K. 2010 The relationship between seed coat color and seed quality in watermelon Crimson sweet Hort. Sci. (Prague). 37 2 62 69 Mayberry, K. 2003

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. Peterson, C.A. Gijzen, M. 2004 Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water Ann. Bot. 94 213 228 10.1093/aob/mch133 Martínez Richart, A.I. 2019 Aloe arborescens . The IUCN red list of threatened species

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distilled water for ≈5 h to soften seedcoats for easy removal and to improve seed germination. Seed coats were removed under sterile conditions and the seeds without seedcoats ( Fig. 1B ) were cultured individually in 25 mm × 150-mm glass test tubes

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Halbert, S.E. 2005 The discovery of huanglongbing in Florida. Proc. of the 2nd International Citrus Canker and Huanglongbing Research Workshop, Orlando, FL. p. 50 Hilf, M. 2011 Colonization of citrus seed coats by ‘ Candidatus Liberibacter asiaticus

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. 2000 Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection Theor. Appl. Genet. 101 146 152 Qu, L.J. Foote, T.N. Roberts, M

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